Germ cell-less (GCL) protein is a nuclear envelope protein highly conserved between the mammalian and Drosophila orthologues.
The information of related literatures
1. S. E. Kleiman, L. Yogev, E. N. Gal-Yam, R. Hauser, R. Gamzu, A. Botchan, G. Paz, H. Yavetz, B. B. Maymon, L. Schreiber, S. Barzilai, N. Amariglio, G. Rechavi and A. J. Simon (2003) Reduced human germ cell-less (HGCL) expression in azoospermic men with severe germinal cell impairment. J Androl 24(5): 670-5.
Abstract Germ cell-less (GCL) protein is a nuclear envelope protein highly conserved between the mammalian and Drosophila orthologues. In Drosophila, maternal GCL protein is required to establish the germ lineage during embryonic development. In mammals, it is suggested that the GCL function is mainly in spermatogenesis and that it might be related to the ability of mouse GCL to repress transcription. Using reverse transcriptase-polymerase chain reaction analyses, we investigated the role of human GCL (HGCL) in spermatogenesis by studying its expression in the testicular tissue of 67 azoospermic men with normal karyotype and no Y-chromosome microdeletion. Their testicular biopsy specimens underwent meticulous histological and cytological analysis as well as molecular analysis with various markers of spermatogenesis (RBM1, DAZ, and CDY1). The rate of X-Y and 18 chromosome bivalent formation during meiosis was additionally assessed in 22 of these biopsy specimens and correlated to HGCL expression. Expression of HGCL was affected in parallel with the severity of testicular impairment found. Defective sperm motility was associated with the absence of HGCL. Nevertheless, the absence of HGCL expression did not influence the normal process of chromosome bivalent formation in meiosis. Our results suggest that HGCL is not essential for the chromosomal events of meiosis but might be involved in later aspects of spermatogenesis. PMID: [12954656]
Figures for illustrating the function of this protein/gene
Function
Possible function in spermatogenesis. Enhances thedegradation of MDM2 and increases the amount of p53 probably bymodulating the nucleocytoplasmic transport (By similarity).
CHAIN 1 515 Germ cell-less protein-like 1. /FTId=PRO_0000087520. DOMAIN 108 178 BTB. MOTIF 49 55 Nuclear localization signal (Potential). MOTIF 85 91 Nuclear localization signal (Potential). CONFLICT 157 157 I -> M (in Ref. 1; BAB14416). CONFLICT 160 160 E -> G (in Ref. 1; BAB14796). CONFLICT 160 160 E -> V (in Ref. 1; BAB14494). CONFLICT 207 207 T -> A (in Ref. 1; BAB14796). CONFLICT 325 325 K -> N (in Ref. 1; BAB14494). Back to Top