SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000439 
UniProt Accession
Theoretical PI
7.1  
Molecular Weight
160970 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
NOS1 
Gene Synonyms/Alias
 
Protein Name
Nitric oxide synthase, brain 
Protein Synonyms/Alias
EC=1.14.13.39 Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS;N-NOSnNOS Peptidyl-cysteine S-nitrosylase NOS1; bNOS; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:12;117636114-117889975;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Increased production of NO in the testis is involved in the enlargement of varicocele and indirectly deteriorates spermatogenesis 
The information of related literatures
1. K. Shiraishi and K. Naito (2007) Nitric oxide produced in the testis is involved in dilatation of the internal spermatic vein that compromises spermatogenesis in infertile men with varicocele. BJU Int 99(5): 1086-90. 

Abstract
OBJECTIVE PMID: [17346270] 

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Figures for illustrating the function of this protein/gene
Function
Produces nitric oxide (NO) which is a messenger moleculewith diverse functions throughout the body. In the brain andperipheral nervous system, NO displays many properties of aneurotransmitter. Probably has nitrosylase activity and mediatescysteine S-nitrosylation of cytoplasmic target proteins such SRR. 
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Subcellular Location
Cell membrane, sarcolemma; Peripheralmembrane protein. Cell projection, dendritic spine (Bysimilarity). Note=In skeletal muscle, it is localized beneath thesarcolemma of fast-twitch muscle fiber by associating with thedystrophin glycoprotein complex. In neurons, enriched in dendriticspines (By similarity). 
Tissue Specificity
Isoform 1 is ubiquitously expressed: detectedin skeletal muscle and brain, also in testis, lung and kidney, andat low levels in heart, adrenal gland and retina. Not detected inthe platelets. Isoform 3 is expressed only in testis. Isoform 4 isdetected in testis, skeletal muscle, lung, and kidney, at lowlevels in the brain, but not in the heart and adrenal gland. 
Gene Ontology
GO IDGO termEvidence
GO:0005856 C:cytoskeleton ISS:BHF-UCL.
GO:0005829 C:cytosol TAS:Reactome.
GO:0043197 C:dendritic spine IEA:UniProtKB-SubCell.
GO:0048471 C:perinuclear region of cytoplasm ISS:BHF-UCL.
GO:0001917 C:photoreceptor inner segment ISS:BHF-UCL.
GO:0042383 C:sarcolemma IDA:BHF-UCL.
GO:0016529 C:sarcoplasmic reticulum IDA:BHF-UCL.
GO:0045202 C:synapse IEA:Compara.
GO:0034618 F:arginine binding TAS:BHF-UCL.
GO:0046870 F:cadmium ion binding ISS:BHF-UCL.
GO:0050660 F:flavin adenine dinucleotide binding ISS:BHF-UCL.
GO:0010181 F:FMN binding ISS:BHF-UCL.
GO:0020037 F:heme binding ISS:BHF-UCL.
GO:0050661 F:NADP binding ISS:BHF-UCL.
GO:0004517 F:nitric-oxide synthase activity ISS:BHF-UCL.
GO:0034617 F:tetrahydrobiopterin binding NAS:BHF-UCL.
GO:0006527 P:arginine catabolic process IC:BHF-UCL.
GO:0007596 P:blood coagulation TAS:Reactome.
GO:0042738 P:exogenous drug catabolic process IEA:Compara.
GO:0033555 P:multicellular organismal response to stress IMP:BHF-UCL.
GO:0007520 P:myoblast fusion TAS:BHF-UCL.
GO:0010523 P:negative regulation of calcium ion transport into cytosol TAS:BHF-UCL.
GO:0051346 P:negative regulation of hydrolase activity IEA:Compara.
GO:0043267 P:negative regulation of potassium ion transport IEA:Compara.
GO:0042136 P:neurotransmitter biosynthetic process TAS:BHF-UCL.
GO:0006809 P:nitric oxide biosynthetic process ISS:BHF-UCL.
GO:0018119 P:peptidyl-cysteine S-nitrosylation IEA:Compara.
GO:0045944 P:positive regulation of transcription from RNA polymerase II promoter IEA:Compara.
GO:0045909 P:positive regulation of vasodilation IDA:BHF-UCL.
GO:0055117 P:regulation of cardiac muscle contraction TAS:BHF-UCL.
GO:0002028 P:regulation of sodium ion transport IEA:Compara.
GO:0009408 P:response to heat IDA:BHF-UCL.
GO:0001666 P:response to hypoxia IEP:BHF-UCL.
GO:0006941 P:striated muscle contraction IEA:Compara.
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Interpro
IPR003097;    FAD-binding_1.
IPR017927;    Fd_Rdtase_FAD-bd.
IPR001094;    Flavdoxin.
IPR008254;    Flavodoxin/NO_synth.
IPR001709;    Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173;    NADPH_Cyt_P450_Rdtase_dom3.
IPR004030;    NO_synthase_oxygenase_dom.
IPR026009;    NOS.
IPR012144;    NOS_met.
IPR001433;    OxRdtase_FAD/NAD-bd.
IPR001478;    PDZ.
IPR017938;    Riboflavin_synthase-like_b-brl.
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Pfam
PF00667;    FAD_binding_1;    1.
PF00258;    Flavodoxin_1;    1.
PF00175;    NAD_binding_1;    1.
PF02898;    NO_synthase;    1.
PF00595;    PDZ;    1.
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SMART
SM00228;    PDZ;    1.
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PROSITE
PS51384;    FAD_FR;    1.
PS50902;    FLAVODOXIN_LIKE;    1.
PS60001;    NOS;    1.
PS50106;    PDZ;    1.
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PRINTS
PR00369;    FLAVODOXIN.;   
PR00371;    FPNCR.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1434       Nitric oxide synthase, brain.
                             /FTId=PRO_0000170921.
DOMAIN       17     99       PDZ.
DOMAIN      760    940       Flavodoxin-like.
DOMAIN      995   1242       FAD-binding FR-type.
NP_BIND     886    917       FMN (By similarity).
NP_BIND    1032   1043       FAD (By similarity).
NP_BIND    1175   1185       FAD (By similarity).
NP_BIND    1250   1268       NADP (By similarity).
NP_BIND    1348   1363       NADP (By similarity).
REGION        1    205       Interaction with NOSIP (By similarity).
REGION      163    245       PIN (nNOS-inhibiting protein) binding.
REGION      730    750       Calmodulin-binding (Potential).
REGION      755    774       Tetrahydrobiopterin-binding (By
                             similarity).
METAL       420    420       Iron (heme axial ligand) (By similarity).
VAR_SEQ       1    336       Missing (in isoform 3).
                             /FTId=VSP_003571.
VAR_SEQ     285    407       PPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKS
                             TLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEF
                             IDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELI
                             -> MRKLRITEGFGVQRGSHNHPPPQENSPPQRMAAPPSVH
                             ASSRSRTGRLRWFSLTPSTLRAHWKRDALSTSAWAPSCILL
                             SMQGGLKTSAQKDSSSLSPKSLLINTIHQLKDLAPKPTWKG
                             WKR (in isoform 4).
                             /FTId=VSP_003572.
VAR_SEQ     408   1434       Missing (in isoform 4).
                             /FTId=VSP_003573.
VAR_SEQ     509    613       Missing (in isoform 2).
                             /FTId=VSP_003574.
VARIANT     228    228       P -> S (in dbSNP:rs9658279).
                             /FTId=VAR_018948.
VARIANT     394    394       D -> A (in dbSNP:rs9658356).
                             /FTId=VAR_018949.
VARIANT     725    725       N -> D (in dbSNP:rs9658403).
                             /FTId=VAR_018950.
VARIANT     864    864       G -> D (in dbSNP:rs9658445).
                             /FTId=VAR_018951.
VARIANT    1064   1064       Q -> R (in dbSNP:rs9658482).
                             /FTId=VAR_018952.
CONFLICT    131    131       K -> E (in Ref. 4; AAB49040).
CONFLICT    178    184       LAPRPPG -> WPQAPR (in Ref. 3 and 4).
CONFLICT    492    493       QP -> HR (in Ref. 3; AAA36376).
CONFLICT    549    549       V -> L (in Ref. 3; AAA36376).
CONFLICT    563    563       G -> A (in Ref. 3; AAA36376).
CONFLICT   1407   1407       Y -> I (in Ref. 3; AAA36376).
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Nucleotide Sequence
Length: 7124 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1434 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Htr2bMINT 
CAMK2AHPRD 
_HPRD 
ARG1HPRD 
CAMK1HPRD 
_BioGRID 
PRKCAHPRD 
_BioGRID 
BDKRB2BioGRID 
NOS1APHPRD 
CAV1BioGRID 
CAV3HPRD 
STUB1BioGRID 
CTBP1HPRD 
DLG2HPRD 
DLG4HPRD 
DLGAP2HPRD 
DYNLL1HPRD 
HSP90AA1HPRD 
PFKMHPRD 
PRKACAHPRD 
CAMK1HPRD 
CAMK4HPRD 
PRKCAHPRD 
ADRA1AHPRD 
ADRA1BHPRD 
ADRA1DHPRD 
ADRB1HPRD 
ADRB2HPRD 
ATP2B4HPRD 
ADRA1AHPRD 
ADRA1DHPRD 
_BioGRID 
BDKRB2HPRD 
NOS1APBioGRID 
STUB1BioGRID 
Ctbp1BioGRID 
DLG2BioGRID 
DLG4BioGRID 
DLG2BioGRID 
HMOX1HPRD 
HSPA4BioGRID 
_BioGRID 
DKFZp781E0954BioGRID 
HMOX1HPRD 
DKFZp434J032HPRD 
UBCBioGRID 
VAC14HPRD 
NOSIPHPRD 
PTPN6HPRD 
PTPRNHPRD 
_BioGRID 
PTPN6BioGRID 
DNCL1HPRD 
PRKCAHPRD 
_HPRD 
CAMK2AHPRD 
PTPRNHPRD 
_HPRD 
RASD1HPRD 
SNTA1HPRD 
SYN1BioGRID 
VAC14MINT 
ZDHHC23HPRD 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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