SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000440 
UniProt Accession
Theoretical PI
6.5  
Molecular Weight
18576 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
CMTM1 
Gene Synonyms/Alias
CKLFSF1 
Protein Name
CKLF-like MARVEL transmembrane domain-containing protein 1 
Protein Synonyms/Alias
Chemokine-like factor superfamily member 1; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:16;66600294-66613040;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
we propose that CKLFSF1 may play an important role in spermatogenesis or testicular development. 
The information of related literatures
1. L. Wang, C. Wu, Y. Zheng, X. Qiu, H. Fan, W. Han, B. Lv, Y. Wang, X. Zhu, M. Xu, P. Ding, S. Cheng, Y. Zhang, Q. Song and D. Ma (2004) Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue. Int J Biochem Cell Biol 36(8): 1492-501. 

Abstract
Chemokine-like factor (CKLF) was isolated from PHA-stimulated U937 cells. It is composed of 152 amino acids and located on chromosome 16q22. Utilizing bioinformatics, based on CKLF cDNA and protein sequences, in combination with experimental validation, we identified a novel gene designated chemokine-like factor super family member 1 (CKLFSF1). CKLFSF1 maps on chromosome 16q22, and the full-length gene comprises of seven exons and six introns. Using RACE-PCR, we identified two potential alternative transcription start sites, 1A and 1B. Northern blot and RT-PCR analysis demonstrated that CKLFSF1 is predominantly expressed in human testis tissue, with only lower levels of expression in many other human tissues. RT-PCR and cDNA sequencing identified 23 alternatively spliced isoforms of CKLFSF1 in the testis tissue, which encode protein variants ranging from 36 to 169 amino acids in length. Immunohistochemistry analysis demonstrated that CKLFSF1 proteins are highly expressed in spermatocyte and in tissue fluid of human testes tissue. In light of these findings, we propose that CKLFSF1 may play an important role in spermatogenesis or testicular development. PMID: [15147728] 

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Figures for illustrating the function of this protein/gene
Function
 
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Subcellular Location
Membrane; Multi-pass membrane protein. 
Tissue Specificity
Highly expressed in testis. 
Gene Ontology
GO IDGO termEvidence
GO:0005615 C:extracellular space IEA:UniProtKB-KW.
GO:0016021 C:integral to membrane IEA:UniProtKB-KW.
GO:0006935 P:chemotaxis IEA:UniProtKB-KW.
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Interpro
IPR008253;    Marvel.
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Pfam
SMART
PROSITE
PS51225;    MARVEL;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    169       CKLF-like MARVEL transmembrane domain-
                             containing protein 1.
                             /FTId=PRO_0000186097.
TRANSMEM     22     42       Helical; (Potential).
TRANSMEM     46     66       Helical; (Potential).
TRANSMEM     79     99       Helical; (Potential).
TRANSMEM    110    130       Helical; (Potential).
DOMAIN       17    135       MARVEL.
VAR_SEQ       1     26       MDPEHAKPESSEAPSGNLKQPETAAA -> MLKILR (in
                             isoform 1a, isoform 1b and isoform 19).
                             /FTId=VSP_008229.
VAR_SEQ       8     65       Missing (in isoform 12).
                             /FTId=VSP_008230.
VAR_SEQ      26     26       A -> ALASSGSVVSSVPKAQRNISAKTAPRKHPAVSIRSA
                             QSAAAARPQGSEGTAPSRKATTRPPPKPTLPPPTPSAHTES
                             KLLNEMAIKERVEGRAKVPYKFRDSLKRFSFSPTGMLKILR
                             (in isoform 24).
                             /FTId=VSP_043579.
VAR_SEQ      28     92       SLILGALACFIITQANESFITITSLEICIVVFFILIYVLTL
                             HHLLTYLHWPLLDLTNSIITAVFL -> ASSGSVVSSVPKA
                             QRNISAKTAPRKHPAVSIRSAQSAAAARPQGSEGTAPSRIL
                             PTVSLQLCSFQ (in isoform 6).
                             /FTId=VSP_008238.
VAR_SEQ      28     80       Missing (in isoform 4 and isoform 5).
                             /FTId=VSP_008237.
VAR_SEQ      28     65       SLILGALACFIITQANESFITITSLEICIVVFFILIYV ->
                             ASSGSVVSSVPKAQRNISAKTAPRKHPETGPDAPQRPA
                             (in isoform 10).
                             /FTId=VSP_008236.
VAR_SEQ      28     38       SLILGALACFI -> EADKANMSNFN (in isoform
                             13).
                             /FTId=VSP_008235.
VAR_SEQ      28     33       SLILGA -> EAYNSK (in isoform 11).
                             /FTId=VSP_008233.
VAR_SEQ      28     33       SLILGA -> ASSGSV (in isoform 3).
                             /FTId=VSP_008234.
VAR_SEQ      28     32       SLILG -> LCSFQ (in isoform 14).
                             /FTId=VSP_008232.
VAR_SEQ      28     28       S -> R (in isoform 16).
                             /FTId=VSP_008231.
VAR_SEQ      29    169       Missing (in isoform 16).
                             /FTId=VSP_008239.
VAR_SEQ      33    169       Missing (in isoform 14).
                             /FTId=VSP_008240.
VAR_SEQ      34    169       Missing (in isoform 11).
                             /FTId=VSP_008241.
VAR_SEQ      34     80       Missing (in isoform 3).
                             /FTId=VSP_008242.
VAR_SEQ      39    169       Missing (in isoform 13).
                             /FTId=VSP_008243.
VAR_SEQ      66    169       Missing (in isoform 10).
                             /FTId=VSP_008244.
VAR_SEQ      77     77       W -> R (in isoform 12).
                             /FTId=VSP_008245.
VAR_SEQ      81    103       DLTNSIITAVFLSVVAILAMQEK -> AAELSLSHRMPSTG
                             SRQSKYEQL (in isoform 12).
                             /FTId=VSP_008246.
VAR_SEQ      81     91       DLTNSIITAVF -> NAEYRKQTKQI (in isoform 8
                             and isoform 19).
                             /FTId=VSP_008247.
VAR_SEQ      92    169       Missing (in isoform 8 and isoform 19).
                             /FTId=VSP_008248.
VAR_SEQ      93    169       Missing (in isoform 6).
                             /FTId=VSP_008249.
VAR_SEQ     104    169       Missing (in isoform 12).
                             /FTId=VSP_008250.
VAR_SEQ     114    114       S -> R (in isoform 2 and isoform 5).
                             /FTId=VSP_008251.
VAR_SEQ     115    169       Missing (in isoform 1b, isoform 2 and
                             isoform 5).
                             /FTId=VSP_008252.
VARIANT      10     10       S -> T (in dbSNP:rs16956746).
                             /FTId=VAR_053039.
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Nucleotide Sequence
Length: 790 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 169 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
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