SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000449 
UniProt Accession
Theoretical PI
6.44  
Molecular Weight
52697 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
TCFL5 
Gene Synonyms/Alias
CHA, E2BP1 
Protein Name
Transcription factor-like 5 protein 
Protein Synonyms/Alias
Cha transcription factor; HPV-16 E2-binding protein 1;E2BP-1 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:20;61472467-61493115;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
The cell-type and stage-specific expression of TCFL5 indicates that this protein may function in a crucial role in spermatogenesis as a transcription factor by regulating cell proliferation or differentiation of cells through binding to a specific DNA sequence like other bHLH molecules. 
The information of related literatures
1. O. Maruyama, H. Nishimori, T. Katagiri, Y. Miki, A. Ueno and Y. Nakamura (1998) Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage. Cytogenet Cell Genet 82(1-2): 41-5. 

Abstract
We have isolated a novel human gene that is expressed specifically in primary spermatocytes in the testis. The cDNA contains an open reading frame of 1356 bp, encoding a 452-amino-acid protein that includes a basic Helix-Loop-Helix (bHLH) motif. The gene, which was mapped to chromosome region 20q13.3-->qter by fluorescence in situ hybridization, consists of six exons and spans approximately 24 kb of genomic DNA. Immunohistochemical staining located the gene product exclusively in cell nuclei of primary spermatocytes at the pachytene stage, but not in those at the leptonema stage. We named this gene TCFL5 (transcription factor-like 5, basic helix-loop-helix). The cell-type and stage-specific expression of TCFL5 indicates that this protein may function in a crucial role in spermatogenesis as a transcription factor by regulating cell proliferation or differentiation of cells through binding to a specific DNA sequence like other bHLH molecules. PMID: [9763657] 

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Figures for illustrating the function of this protein/gene
Function
Putative transcription factor. Isoform 3 may play a rolein early spermatogenesis. 
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Subcellular Location
Nucleus. 
Tissue Specificity
Isoform 3 is testis specific. Isoform 2 ispancreas specific. 
Gene Ontology
GO IDGO termEvidence
GO:0001673 C:male germ cell nucleus IEA:Compara.
GO:0005634 C:nucleus IDA:UniProtKB.
GO:0003677 F:DNA binding IDA:UniProtKB.
GO:0003700 F:sequence-specific DNA binding transcription factor activity TAS:ProtInc.
GO:0030154 P:cell differentiation IEA:UniProtKB-KW.
GO:0007275 P:multicellular organismal development IEA:UniProtKB-KW.
GO:0045595 P:regulation of cell differentiation IEP:UniProtKB.
GO:0042127 P:regulation of cell proliferation IEP:UniProtKB.
GO:0007283 P:spermatogenesis IEP:UniProtKB.
GO:0006366 P:transcription from RNA polymerase II promoter TAS:ProtInc.
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Interpro
IPR011598;    HLH_dom.
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Pfam
PF00010;    HLH;    1.
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SMART
SM00353;    HLH;    1.
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PROSITE
PS50888;    BHLH;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    500       Transcription factor-like 5 protein.
                             /FTId=PRO_0000127475.
DOMAIN      400    450       bHLH.
REGION      347    356       R3 epitope (recognized by Chagas's
                             antibodies).
REGION      481    500       R1 epitope (recognized by Chagas's
                             antibodies).
COMPBIAS     67    182       Ala-rich.
VAR_SEQ       1    227       Missing (in isoform 2).
                             /FTId=VSP_002160.
VAR_SEQ       1     48       Missing (in isoform 1).
                             /FTId=VSP_030310.
VARIANT     272    272       N -> D (in dbSNP:rs17854409).
                             /FTId=VAR_061263.
VARIANT     380    380       E -> D (in dbSNP:rs34304654).
                             /FTId=VAR_049555.
CONFLICT     75     75       T -> M (in Ref. 4; BAA36557).
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Nucleotide Sequence
Length: 1458 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 500 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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