SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000464 
UniProt Accession
Theoretical PI
5.71  
Molecular Weight
54692 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
DDX25 
Gene Synonyms/Alias
GRTH 
Protein Name
ATP-dependent RNA helicase DDX25 
Protein Synonyms/Alias
EC=3.6.4.13 DEAD box protein 25; Gonadotropin-regulated testicular RNA helicase; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:11;125773271-125793158;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
SNP IVS6+55G-->T and c.852C-->T of GRTH gene may be associated with male infertility with azoospermia or severe oligozoospermia, suggesting that variations in GRTH gene may contribute to susceptibility to spermatogenic impairment in humans. 
The information of related literatures
1. Z. A, S. Zhang, Y. Yang, Y. Ma, L. Lin and W. Zhang (2006) Single nucleotide polymorphisms of the gonadotrophin-regulated testicular helicase (GRTH) gene may be associated with the human spermatogenesis impairment. Hum Reprod 21(3): 755-9. 

Abstract
BACKGROUND PMID: [16293649] 

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Figures for illustrating the function of this protein/gene
Function
ATP-dependent RNA helicase. Required for mRNA export andtranslation regulation during spermatid development (Bysimilarity). 
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Subcellular Location
Cytoplasm. Nucleus (By similarity).Note=Detected in both cytoplasm and nucleus of testicular cells.Also detected in chromatoid bodies of round spermatids (Bysimilarity). 
Tissue Specificity
Highly expressed in the Leydig and germ cellsof the testis and weakly expressed in the pituitary andhypothalamus. 
Gene Ontology
GO IDGO termEvidence
GO:0033391 C:chromatoid body ISS:UniProtKB.
GO:0005634 C:nucleus ISS:UniProtKB.
GO:0005524 F:ATP binding ISS:UniProtKB.
GO:0004004 F:ATP-dependent RNA helicase activity ISS:UniProtKB.
GO:0003723 F:RNA binding IEA:UniProtKB-KW.
GO:0006406 P:mRNA export from nucleus ISS:UniProtKB.
GO:0007275 P:multicellular organismal development IEA:UniProtKB-KW.
GO:0006417 P:regulation of translation ISS:UniProtKB.
GO:0007286 P:spermatid development ISS:UniProtKB.
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Interpro
IPR011545;    DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001;    Helicase_ATP-bd.
IPR001650;    Helicase_C.
IPR014014;    RNA_helicase_DEAD_Q_motif.
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Pfam
PF00270;    DEAD;    1.
PF00271;    Helicase_C;    1.
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SMART
SM00487;    DEXDc;    1.
SM00490;    HELICc;    1.
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PROSITE
PS00039;    DEAD_ATP_HELICASE;    FALSE_NEG.
PS51192;    HELICASE_ATP_BIND_1;    1.
PS51194;    HELICASE_CTER;    1.
PS51195;    Q_MOTIF;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    483       ATP-dependent RNA helicase DDX25.
                             /FTId=PRO_0000030813.
DOMAIN      130    300       Helicase ATP-binding.
DOMAIN      311    478       Helicase C-terminal.
NP_BIND     143    150       ATP (By similarity).
MOTIF        61     74       Nuclear export signal (By similarity).
MOTIF        97    125       Q motif.
MOTIF       100    114       Nuclear localization signal (By
                             similarity).
MOTIF       247    250       DEAD box.
VAR_SEQ       1    114       Missing (in isoform 2).
                             /FTId=VSP_018875.
CONFLICT     21     21       H -> N (in Ref. 1; AAF21371/AAU84667).
CONFLICT    381    381       I -> T (in Ref. 1; AAF21371/AAU84667).
STRAND      312    318
HELIX       322    333
STRAND      339    344
HELIX       348    359
TURN        360    362
STRAND      365    368
HELIX       374    385
STRAND      390    394
TURN        400    402
STRAND      407    414
HELIX       425    432
STRAND      442    448
HELIX       450    452
HELIX       453    463
STRAND      468    470
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Nucleotide Sequence
Length: 1744 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 483 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
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