SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000467 
UniProt Accession
Theoretical PI
6.11  
Molecular Weight
34620 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
NANOG 
Gene Synonyms/Alias
 
Protein Name
Homeobox protein NANOG 
Protein Synonyms/Alias
Homeobox transcription factor Nanog;hNanog 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:12;7940390-7948655;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
The findings of the current study strongly suggest a conserved role for NANOG in meiotic and post-meiotic stages of male germ cell development. 
The information of related literatures
1. E. W. Kuijk, J. de Gier, S. M. Lopes, I. Chambers, A. M. van Pelt, B. Colenbrander and B. A. Roelen (2010) A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis. PLoS One 5(6): e10987. 

Abstract
BACKGROUND PMID: [20539761] 

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Figures for illustrating the function of this protein/gene
Ref: E. W. Kuijk, J. de Gier, S. M. Lopes, I. Chambers, A. M. van Pelt, B. Colenbrander and B. A. Roelen (2010) A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis. PLoS One 5(6): e10987. PMID: [20539761]
Ref: E. W. Kuijk, J. de Gier, S. M. Lopes, I. Chambers, A. M. van Pelt, B. Colenbrander and B. A. Roelen (2010) A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis. PLoS One 5(6): e10987. PMID: [20539761]
Ref: E. W. Kuijk, J. de Gier, S. M. Lopes, I. Chambers, A. M. van Pelt, B. Colenbrander and B. A. Roelen (2010) A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis. PLoS One 5(6): e10987. PMID: [20539761]
Ref: E. W. Kuijk, J. de Gier, S. M. Lopes, I. Chambers, A. M. van Pelt, B. Colenbrander and B. A. Roelen (2010) A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis. PLoS One 5(6): e10987. PMID: [20539761]
Function
Transcription regulator involved in inner cell mass andembryonic stem (ES) cells proliferation and self-renewal. Imposespluripotency on ES cells and prevents their differentiationtowards extraembryonic endoderm and trophectoderm lineages. Blocksbone morphogenetic protein-induced mesoderm differentiation of EScells by physically interacting with SMAD1 and interfering withthe recruitment of coactivators to the active SMAD transcriptionalcomplexes (By similarity). Acts as a transcriptional activator orrepressor (By similarity). Binds optimally to the DNA consensussequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'(By similarity). When overexpressed, promotes cells to enter intoS phase and proliferation (By similarity). 
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Subcellular Location
Nucleus. 
Tissue Specificity
Expressed in testicular carcinoma and derivedgerm cell tumors (at protein level). Expressed in fetal gonads,ovary and testis. Also expressed in ovary teratocarcinoma cellline and testicular embryonic carcinoma. Not expressed in manysomatic organs and oocytes. 
Gene Ontology
GO IDGO termEvidence
GO:0005730 C:nucleolus IDA:BHF-UCL.
GO:0003677 F:DNA binding IDA:UniProtKB.
GO:0043565 F:sequence-specific DNA binding IEA:InterPro.
GO:0003700 F:sequence-specific DNA binding transcription factor activity IDA:HGNC.
GO:0008283 P:cell proliferation IMP:HGNC.
GO:0001714 P:endodermal cell fate specification IDA:MGI.
GO:0045944 P:positive regulation of transcription from RNA polymerase II promoter IDA:MGI.
GO:0035019 P:somatic stem cell maintenance IMP:UniProtKB.
GO:0006351 P:transcription, DNA-dependent IEA:UniProtKB-KW.
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Interpro
IPR017970;    Homeobox_CS.
IPR001356;    Homeodomain.
IPR009057;    Homeodomain-like.
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Pfam
PF00046;    Homeobox;    1.
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SMART
SM00389;    HOX;    1.
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PROSITE
PS00027;    HOMEOBOX_1;    1.
PS50071;    HOMEOBOX_2;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    305       Homeobox protein NANOG.
                             /FTId=PRO_0000261418.
REPEAT      196    200       1.
REPEAT      201    205       2.
REPEAT      206    210       3.
REPEAT      216    220       4.
REPEAT      221    225       5.
REPEAT      226    230       6.
REPEAT      231    235       7.
REPEAT      236    240       8.
DNA_BIND     95    154       Homeobox.
REGION      196    240       8 X repeats starting with a Trp in each
                             unit.
REGION      196    240       Sufficient for transactivation activity
                             (By similarity).
REGION      241    305       Sufficient for strong transactivation
                             activity (By similarity).
VAR_SEQ     168    183       Missing (in isoform 2).
                             /FTId=VSP_021688.
VARIANT      82     82       K -> N (in dbSNP:rs2889551).
                             /FTId=VAR_029384.
HELIX       104    116
STRAND      117    119
HELIX       122    131
HELIX       136    148
TURN        149    151
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Nucleotide Sequence
Length: 2114 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 305 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
MED12BioGRID 
MED12BioGRID 
RHOXF2BioGRID 
SALL4BioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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