SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000505 
UniProt Accession
Theoretical PI
8.72  
Molecular Weight
37465 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
MAS1 
Gene Synonyms/Alias
MAS 
Protein Name
Proto-oncogene Mas 
Protein Synonyms/Alias
 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:6;160327974-160329108;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
The immunolocalization of Ang-(1-7) and its receptor Mas in testes of fertile and infertile men, and suggests that this system may be altered when spermatogenesis is severely impaired. 
The information of related literatures
1. A. B. Reis, F. C. Araujo, V. M. Pereira, A. M. Dos Reis, R. A. Santos and F. M. Reis (2010) Angiotensin (1-7) and its receptor Mas are expressed in the human testis. J Mol Histol 41(1): 75-80. 

Abstract
The presence of classical components of the renin-angiotensin system has been demonstrated in the male reproductive tract, mainly in the testes and epididymis. The objective of this study was to verify the localization of angiotensin (Ang)-(1-7) and its receptor Mas in human testis. The study included 12 men with previously proven fertility submitted to orchiectomy for prostate cancer and 20 infertile men submitted to testicular biopsy for infertility work-up, comprising a subgroup with obstructive azoospermia/normal spermatogenesis (n = 8) and another with non-obstructive azoospermia and severely impaired spermatogenesis (n = 12). Testicular tissue samples were processed by immunohistochemistry and real time polymerase chain reaction. Ang-(1-7) was strongly expressed in the interstitial compartment, mainly in Leydig cells, with similar intensity in all groups evaluated. The peptide was also detected in the seminiferous tubules, but with much less intensity compared to interstitial cells. The receptor Mas was equally distributed between interstitial and tubular compartments and was found in all layers of the normal seminiferous epithelium. However, neither Ang-(1-7) nor Mas were detected in the seminiferous tubules of samples with impaired spermatogenesis. The testicular samples of infertile men with impaired spermatogenesis (non-obstructive azoospermia) expressed Mas and ACE2 mRNA at lower concentrations (fold change = 0.06 and 0.04, respectively, P < 0.05) than samples with full spermatogenesis (obstructive azoospermia). This shows, for the first time, the immunolocalization of Ang-(1-7) and its receptor Mas in testes of fertile and infertile men, and suggests that this system may be altered when spermatogenesis is severely impaired. PMID: [20361351] 

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Figures for illustrating the function of this protein/gene
Function
Receptor for angiotensin 1-7 (By similarity). Actsspecifically as a functional antagonist of AGTR1 (angiotensin-2type 1 receptor), although it up-regulates AGTR1 receptor levels.Positive regulation of AGTR1 levels occurs through activation ofthe G-proteins GNA11 and GNAQ, and stimulation of the proteinkinase C signaling cascade. The antagonist effect on AGTR1function is probably due to AGTR1 being physically altered byMAS1. 
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Subcellular Location
Cell membrane; Multi-pass membrane protein. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0009986 C:cell surface IEA:Compara.
GO:0005887 C:integral to plasma membrane TAS:ProtInc.
GO:0004945 F:angiotensin type II receptor activity TAS:ProtInc.
GO:0042277 F:peptide binding ISS:UniProtKB.
GO:0017046 F:peptide hormone binding IEA:Compara.
GO:0007250 P:activation of NF-kappaB-inducing kinase activity IEA:Compara.
GO:0009653 P:anatomical structure morphogenesis TAS:ProtInc.
GO:0008283 P:cell proliferation TAS:ProtInc.
GO:0071375 P:cellular response to peptide hormone stimulus IEA:Compara.
GO:0021766 P:hippocampus development IEA:Compara.
GO:0008584 P:male gonad development IEA:Compara.
GO:0001933 P:negative regulation of protein phosphorylation IEA:Compara.
GO:0008284 P:positive regulation of cell proliferation IEA:Compara.
GO:0045740 P:positive regulation of DNA replication IEA:Compara.
GO:0060732 P:positive regulation of inositol phosphate biosynthetic process IEA:Compara.
GO:0070528 P:protein kinase C signaling cascade IMP:UniProtKB.
GO:0050727 P:regulation of inflammatory response IEA:Compara.
GO:0014823 P:response to activity IEA:Compara.
GO:0042493 P:response to drug IEA:Compara.
GO:0034698 P:response to gonadotropin stimulus IEA:Compara.
GO:0007283 P:spermatogenesis IEA:Compara.
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Interpro
IPR000276;    7TM_GPCR_Rhodpsn.
IPR017452;    GPCR_Rhodpsn_supfam.
IPR000820;    Proto-oncogene_Mas.
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Pfam
PF00001;    7tm_1;    1.
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SMART
PROSITE
PS00237;    G_PROTEIN_RECEP_F1_1;    1.
PS50262;    G_PROTEIN_RECEP_F1_2;    1.
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PRINTS
PR00237;    GPCRRHODOPSN.;   
PR00533;    MASONCOGENE.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    325       Proto-oncogene Mas.
                             /FTId=PRO_0000069714.
TOPO_DOM      1     36       Extracellular (Potential).
TRANSMEM     37     61       Helical; Name=1; (Potential).
TOPO_DOM     62     65       Cytoplasmic (Potential).
TRANSMEM     66     86       Helical; Name=2; (Potential).
TOPO_DOM     87    104       Extracellular (Potential).
TRANSMEM    105    128       Helical; Name=3; (Potential).
TOPO_DOM    129    149       Cytoplasmic (Potential).
TRANSMEM    150    172       Helical; Name=4; (Potential).
TOPO_DOM    173    185       Extracellular (Potential).
TRANSMEM    186    206       Helical; Name=5; (Potential).
TOPO_DOM    207    224       Cytoplasmic (Potential).
TRANSMEM    225    245       Helical; Name=6; (Potential).
TOPO_DOM    246    263       Extracellular (Potential).
TRANSMEM    264    284       Helical; Name=7; (Potential).
TOPO_DOM    285    325       Cytoplasmic (Potential).
CARBOHYD      5      5       N-linked (GlcNAc...) (Potential).
CARBOHYD     16     16       N-linked (GlcNAc...) (Potential).
CARBOHYD     22     22       N-linked (GlcNAc...) (Potential).
MUTAGEN     138    138       I->D: Fails to activate GNA11.
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Nucleotide Sequence
Length: 1388 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 325 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
AGTHPRD 
AGTHPRD 
_HPRD 
Other Protein-Protein interaction resources
String database  
View Microarray data
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