SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000511 
UniProt Accession
Theoretical PI
5.27  
Molecular Weight
33253 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
RGN 
Gene Synonyms/Alias
SMP30 
Protein Name
Regucalcin 
Protein Synonyms/Alias
RC Gluconolactonase;GNLEC=3.1.1.17 Senescence marker protein 30;SMP-30 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:X;46937775-46952712;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
RGN is a novel androgen-target gene in rat testis and that it may have a role in male reproductive function, particularly in the control of spermatogenesis. 
The information of related literatures
1. S. S. Laurentino, S. Correia, J. E. Cavaco, P. F. Oliveira, L. Rato, M. Sousa, A. Barros and S. Socorro (2011) Regucalcin is broadly expressed in male reproductive tissues and a new androgen target gene in mammalian testis. Reproduction (): . 

Abstract
Regucalcin (RGN) is a calcium (Ca2+)-binding protein which regulates intracellular Ca2+ homeostasis by modulating the activity of enzymes regulating Ca2+ concentration and enhancing Ca2+-pumping activity. Several studies have described the pivotal role of proper Ca2+ homeostasis regulation to spermatogenesis and male fertility. Recently RGN was identified as a sex steroid regulated gene in prostate and breast however, a possible role of RGN in spermatogenesis has not been examined. In the present study the expression and localization of RGN in rat and human testis, and other rat reproductive tissues was analysed. Moreover, we studied whether RGN protein was present in seminiferous tubule fluid. Finally, we examined the effect of 5alpha-dihydrotestosterone on the expression of Rgn mRNA in rat seminiferous tubules cultured ex vivo. The results presented herein show that RGN is expressed in Leydig and Sertoli cells, as well as in all types of germ cells of both rat and human testis. Regucalcin is also expressed in rat prostate, epididymis, and seminal vesicles. Moreover RGN protein is present in rat seminiferous tubule fluid. The results also demonstrate that RGN expression is age dependent in rat testis, and is up-regulated by the non-aromatizable androgen 5alpha-dihydrotestosterone in rat seminiferous tubules cultured ex vivo. Taken together, these findings indicate that RGN is a novel androgen-target gene in rat testis and that it may have a role in male reproductive function, particularly in the control of spermatogenesis. PMID: [21680783] 

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Figures for illustrating the function of this protein/gene
Function
Gluconolactonase with low activity towards other sugarlactones, including gulonolactone and galactonolactone. Can alsohydrolyze diisopropyl phosphorofluoridate and phenylacetate (invitro). Calcium-binding protein. Modulates Ca(2+) signaling, andCa(2+)-dependent cellular processes and enzyme activities (Bysimilarity). 
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Subcellular Location
Cytoplasm (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm ISS:UniProtKB.
GO:0005634 C:nucleus ISS:UniProtKB.
GO:0005509 F:calcium ion binding IDA:UniProtKB.
GO:0030234 F:enzyme regulator activity IEA:InterPro.
GO:0004341 F:gluconolactonase activity IDA:UniProtKB.
GO:0008270 F:zinc ion binding IDA:UniProtKB.
GO:0006874 P:cellular calcium ion homeostasis ISS:UniProtKB.
GO:0032781 P:positive regulation of ATPase activity ISS:UniProtKB.
GO:0050848 P:regulation of calcium-mediated signaling ISS:UniProtKB.
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Interpro
IPR011042;    6-blade_b-propeller_TolB-like.
IPR008367;    Regucalcin.
IPR013658;    SGL.
IPR005511;    SMP-30.
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Pfam
PF08450;    SGL;    1.
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SMART
PROSITE
PRINTS
PR01791;    REGUCALCIN.;   
PR01790;    SMP30FAMILY.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    299       Regucalcin.
                             /FTId=PRO_0000173046.
METAL        18     18       Divalent metal cation.
METAL       154    154       Divalent metal cation.
METAL       204    204       Divalent metal cation.
MOD_RES       3      3       Phosphoserine (By similarity).
VAR_SEQ     116    187       Missing (in isoform 2).
                             /FTId=VSP_025456.
VAR_SEQ     188    188       S -> A (in isoform 2).
                             /FTId=VSP_025457.
MUTAGEN      18     18       E->A: Reduces enzyme activity by about
                             90%.
MUTAGEN     103    103       N->A: Reduces enzyme activity by about
                             95%.
MUTAGEN     154    154       N->A: Reduces enzyme activity by about
                             95%.
MUTAGEN     204    204       D->A: Reduces enzyme activity by over
                             98%.
CONFLICT    292    293       IA -> TS (in Ref. 4; BAD97080).
STRAND        5     10
STRAND       15     23
TURN         24     27
STRAND       28     33
TURN         34     37
STRAND       38     43
TURN         44     46
STRAND       49     53
STRAND       58     64
STRAND       67     73
STRAND       76     81
TURN         82     85
STRAND       86     92
STRAND       98    107
STRAND      113    119
STRAND      132    137
STRAND      143    158
STRAND      164    169
HELIX       170    172
STRAND      174    180
TURN        182    184
STRAND      187    195
HELIX       198    200
STRAND      202    209
STRAND      214    219
TURN        220    222
STRAND      223    227
TURN        229    231
STRAND      234    239
STRAND      241    243
STRAND      245    252
HELIX       253    255
STRAND      257    263
HELIX       269    274
TURN        276    279
STRAND      281    285
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Nucleotide Sequence
Length: 1356 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 299 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
NR1D2HPRD 
NR1D2HPRD 
POLDIP3String 
POLDIP3String 
THRAHPRD 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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