SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000514 
UniProt Accession
Theoretical PI
8.08  
Molecular Weight
147163 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
RGS22 
Gene Synonyms/Alias
 
Protein Name
Regulator of G-protein signaling 22 
Protein Synonyms/Alias
RGS22 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:8;100973164-101143496;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Their functions in this subcellular compartment are likely related to the postmeiotic developmental phase, spermiogenesis. RGS22 may also play a role in GNA13 translocation from the cytoplasm to the nucleus during spermiogenesis. 
The information of related literatures
1. Y. Hu, J. Xing, L. Chen, X. Guo, Y. Du, C. Zhao, Y. Zhu, M. Lin, Z. Zhou and J. Sha (2008) RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits. Biol Reprod 79(6): 1021-9. 

Abstract
The heterotrimeric G-protein pathway controls numerous cellular processes, including proliferation, differentiation, migration, membrane trafficking, and embryonic development. Regulator of G-protein signaling (RGS) proteins are known to function at the G-protein level. Here, the functional role of a novel RGS protein, regulator of G-protein signaling 22 (RGS22), in the testis was investigated at the mRNA and protein levels. Our results demonstrate that RGS22 is a testis-specific gene. However, significantly decreased expression of RGS22 was found in the testes of patients with azoospermia. RGS22 was translated or posttranslationally modified into multiple proteins of different molecular sizes in prokaryocytes as well as in the testes. Its protein (NP_056483) was localized in spermatogenic cells and Leydig cells and could interact with guanine nucleotide binding protein, alpha 12, 13, and 11 (GNA12, GNA13, and GNA11). Fragmental GFP-fusion protein tracking revealed that the N-terminal of RGS22 was localized in the nucleus. RGS22 and GNA13 were localized in the nucleus from the elongated spermatid stage onward. Indirect immunofluorescence studies revealed defective expression of GNA13 in macrocephalic and global nucleus spermatozoa. These findings suggest that their functions in this subcellular compartment are likely related to the postmeiotic developmental phase, spermiogenesis. RGS22 may also play a role in GNA13 translocation from the cytoplasm to the nucleus during spermiogenesis. PMID: [18703424] 

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Figures for illustrating the function of this protein/gene
Ref: Y. Hu, J. Xing, L. Chen, X. Guo, Y. Du, C. Zhao, Y. Zhu, M. Lin, Z. Zhou and J. Sha (2008) RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits. Biol Reprod 79(6): 1021-9. PMID: [18703424]
Ref: Y. Hu, J. Xing, L. Chen, X. Guo, Y. Du, C. Zhao, Y. Zhu, M. Lin, Z. Zhou and J. Sha (2008) RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits. Biol Reprod 79(6): 1021-9. PMID: [18703424]
Ref: Y. Hu, J. Xing, L. Chen, X. Guo, Y. Du, C. Zhao, Y. Zhu, M. Lin, Z. Zhou and J. Sha (2008) RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits. Biol Reprod 79(6): 1021-9. PMID: [18703424]
Function
Inhibits signal transduction by increasing the GTPaseactivity of G protein alpha subunits thereby driving them intotheir inactive GDP-bound form (By similarity). 
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Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IBA:RefGenome.
GO:0005886 C:plasma membrane IBA:RefGenome.
GO:0005096 F:GTPase activator activity IBA:RefGenome.
GO:0038032 P:termination of G-protein coupled receptor signaling pathway IEA:InterPro.
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Interpro
IPR000342;    Regulat_G_prot_signal.
IPR016137;    Regulat_G_prot_signal_superfam.
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Pfam
PF00615;    RGS;    3.
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SMART
SM00315;    RGS;    2.
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PROSITE
PS50132;    RGS;    2.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1264       Regulator of G-protein signaling 22.
                             /FTId=PRO_0000271376.
DOMAIN      852    980       RGS 1.
DOMAIN     1021   1145       RGS 2.
COILED     1142   1174       Potential.
COMPBIAS    267    277       Poly-Glu.
COMPBIAS    767    771       Poly-Leu.
VAR_SEQ     505   1264       Missing (in isoform 2).
                             /FTId=VSP_022306.
VARIANT     397    397       R -> M (in dbSNP:rs2446927).
                             /FTId=VAR_051797.
VARIANT     943    943       H -> Y (in dbSNP:rs3133711).
                             /FTId=VAR_051798.
CONFLICT     86     86       Missing (in Ref. 1; AAH92411).
CONFLICT    131    131       A -> P (in Ref. 1; AAH47060).
CONFLICT    378    378       E -> K (in Ref. 1; AAH36665).
CONFLICT    995    997       LQK -> DAW (in Ref. 2; CAB55986).
CONFLICT   1233   1233       K -> KK (in Ref. 2; CAB55986).
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Nucleotide Sequence
Length: 4064 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1264 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
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