SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000517 
UniProt Accession
Theoretical PI
5.83  
Molecular Weight
265405 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
NOTCH2 
Gene Synonyms/Alias
 
Protein Name
Neurogenic locus notch homolog protein 2 Notch 2 extracellular truncation Notch 2 intracellular domain 
Protein Synonyms/Alias
Notch 2hN2Contains:Contains:Flags: Precursor 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:1;120454176-120612240;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
In conclusion, the results presented in this study offer the possibility that Notch 1/Jagged 2 signaling system plays an important role for male germ cells to differentiate or at least to survive in the rat testis and fails to express in the testis of spermatogenic maturation arrest patients. 
The information of related literatures
1. T. Hayashi, Y. Kageyama, K. Ishizaka, G. Xia, K. Kihara and H. Oshima (2001) Requirement of Notch 1 and its ligand jagged 2 expressions for spermatogenesis in rat and human testes. J Androl 22(6): 999-1011. 

Abstract
It has already been demonstrated that the Notch signaling system is essential for gametogenesis in the adult germ line of Caenorhabditis elegans. However, the role of the Notch signaling system in mammalian spermatogenesis has not been well investigated. Recently, it has been revealed that this signaling system is expressed in the mammalian testis by showing coexpression of Jagged 2 and its receptor, Notch 1, is consistent with Notch 1 being a cognate receptor for Jagged 2 in the mammalian testis. Therefore, we investigated expressions of messenger RNAs of Notch 1 and Jagged 2 in the testicular tissues of developing Sprague-Dawley rats by reverse transcription-polymerase chain reaction and Northern blot analysis, expressions of their proteins in the testicular tissues of developing rats, fertile human controls and infertile human patients with maturation arrest by immunohistochemistry, and effects of antibodies to this system by culturing rat testicular tissues with these antibodies. Transcripts of Notch 1 and Jagged 2 in the rat testis were positive throughout the examined period; these intensities became higher at day 13 after birth, coincidence with the formation of spermatocytes, and peaked at day 19 after birth. Expressions of Notch 1 and Jagged 2 were recognized at first in the perinuclear regions of spermatocytes in the rat testis as a round structure at day 19 after birth and thereafter in further differentiated germ cells as meiosis proceeded. In the adult rat testis, positive staining was present as a round structure in spermatocytes, as a typical horseshoe-shaped structure in round spermatids, and as a covering structure spreading around the nucleus of elongated spermatids, but not in spermatozoa. Notch 1 was recognized in the vacuole of the Golgi complex of primary spermatocytes and the acrosome of elongated spermatids with electron microscopy. When rat testicular tissues were cultured with anti-Notch 1 or anti-Jagged 2 antibody, round and elongated spermatids decreased after 5 and 7 days of culture, respectively, and disappeared at around 9 and 12 days of culture, respectively, with shrinkage of the diameter of seminiferous tubules. Spermatocytes, however, increased after 11 days of culture. Expressions of both proteins have been detected in the testicular tissues of human fertile controls as in the rat testicular tissues. However, Notch 1 expression has not been detected in testicular tissues of 11 patients with maturation arrest, whereas Jagged 2 expression has been recognized in all of them. In conclusion, the results presented in this study offer the possibility that Notch 1/Jagged 2 signaling system plays an important role for male germ cells to differentiate or at least to survive in the rat testis and fails to express in the testis of spermatogenic maturation arrest patients. PMID: [11700865] 

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Figures for illustrating the function of this protein/gene
Function
Functions as a receptor for membrane-bound ligandsJagged1, Jagged2 and Delta1 to regulate cell-fate determination.Upon ligand activation through the released notch intracellulardomain (NICD) it forms a transcriptional activator complex withRBPJ/RBPSUH and activates genes of the enhancer of split locus.Affects the implementation of differentiation, proliferation andapoptotic programs (By similarity). Involved in bone remodelingand homeostasis. In collaboration with RELA/p65 enhances NFATc1promoter activity and positively regulates RANKL-inducedosteoclast differentiation. 
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Subcellular Location
Notch 2 intracellular domain: Nucleus.Note=Following proteolytical processing NICD is translocated tothe nucleus. 
Tissue Specificity
Expressed in the brain, heart, kidney, lung,skeletal muscle and liver. Ubiquitously expressed in the embryo. 
Gene Ontology
GO IDGO termEvidence
GO:0009986 C:cell surface IDA:UniProtKB.
GO:0005829 C:cytosol TAS:Reactome.
GO:0005789 C:endoplasmic reticulum membrane TAS:Reactome.
GO:0005576 C:extracellular region TAS:Reactome.
GO:0000139 C:Golgi membrane TAS:Reactome.
GO:0005887 C:integral to plasma membrane IDA:UniProtKB.
GO:0005654 C:nucleoplasm TAS:Reactome.
GO:0005509 F:calcium ion binding IEA:InterPro.
GO:0004872 F:receptor activity NAS:UniProtKB.
GO:0006916 P:anti-apoptosis TAS:UniProtKB.
GO:0060413 P:atrial septum morphogenesis IMP:BHF-UCL.
GO:0046849 P:bone remodeling IMP:UniProtKB.
GO:0007050 P:cell cycle arrest IDA:UniProtKB.
GO:0001709 P:cell fate determination TAS:UniProtKB.
GO:0016049 P:cell growth IDA:UniProtKB.
GO:0007368 P:determination of left/right symmetry IEA:Compara.
GO:0030326 P:embryonic limb morphogenesis IEA:Compara.
GO:0030097 P:hemopoiesis TAS:UniProtKB.
GO:0006959 P:humoral immune response IEA:Compara.
GO:0006917 P:induction of apoptosis TAS:UniProtKB.
GO:0002437 P:inflammatory response to antigenic stimulus IEA:Compara.
GO:0072602 P:interleukin-4 secretion IEA:Compara.
GO:0002011 P:morphogenesis of an epithelial sheet IEA:Compara.
GO:0008285 P:negative regulation of cell proliferation IDA:UniProtKB.
GO:0007399 P:nervous system development NAS:UniProtKB.
GO:0007220 P:Notch receptor processing TAS:Reactome.
GO:0061314 P:Notch signaling involved in heart development IC:BHF-UCL.
GO:0046579 P:positive regulation of Ras protein signal transduction IDA:UniProtKB.
GO:0006355 P:regulation of transcription, DNA-dependent TAS:UniProtKB.
GO:0019827 P:stem cell maintenance TAS:UniProtKB.
GO:0042246 P:tissue regeneration IEA:Compara.
GO:0006367 P:transcription initiation from RNA polymerase II promoter TAS:Reactome.
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Interpro
IPR002110;    Ankyrin_rpt.
IPR020683;    Ankyrin_rpt-contain_dom.
IPR024600;    DUF3454_notch.
IPR000742;    EG-like_dom.
IPR006210;    EGF-like.
IPR001881;    EGF-like_Ca-bd.
IPR013032;    EGF-like_CS.
IPR006209;    EGF-like_dom.
IPR000152;    EGF-type_Asp/Asn_hydroxyl_site.
IPR018097;    EGF_Ca-bd_CS.
IPR008297;    Notch.
IPR022336;    Notch_2.
IPR000800;    Notch_dom.
IPR010660;    Notch_NOD_dom.
IPR011656;    Notch_NODP_dom.
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Pfam
PF00023;    Ank;    1.
PF12796;    Ank_2;    2.
PF11936;    DUF3454;    1.
PF00008;    EGF;    23.
PF07645;    EGF_CA;    5.
PF06816;    NOD;    1.
PF07684;    NODP;    1.
PF00066;    Notch;    3.
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SMART
SM00248;    ANK;    6.
SM00181;    EGF;    12.
SM00179;    EGF_CA;    23.
SM00004;    NL;    3.
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PROSITE
PS50297;    ANK_REP_REGION;    1.
PS50088;    ANK_REPEAT;    4.
PS00010;    ASX_HYDROXYL;    22.
PS00022;    EGF_1;    34.
PS01186;    EGF_2;    29.
PS50026;    EGF_3;    35.
PS01187;    EGF_CA;    22.
PS50258;    LNR;    3.
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PRINTS
PR01452;    LNOTCHREPEAT.;   
PR01985;    NOTCH2.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
SIGNAL        1     25       Potential.
CHAIN        26   2471       Neurogenic locus notch homolog protein 2.
                             /FTId=PRO_0000007683.
CHAIN      1666   2471       Notch 2 extracellular truncation (By
                             similarity).
                             /FTId=PRO_0000007684.
CHAIN      1697   2471       Notch 2 intracellular domain (By
                             similarity).
                             /FTId=PRO_0000007685.
TOPO_DOM     26   1677       Extracellular (Potential).
TRANSMEM   1678   1698       Helical; (Potential).
TOPO_DOM   1699   2471       Cytoplasmic (Potential).
DOMAIN       26     63       EGF-like 1.
DOMAIN       64    102       EGF-like 2.
DOMAIN      105    143       EGF-like 3.
DOMAIN      144    180       EGF-like 4.
DOMAIN      182    219       EGF-like 5; calcium-binding (Potential).
DOMAIN      221    258       EGF-like 6.
DOMAIN      260    296       EGF-like 7; calcium-binding (Potential).
DOMAIN      298    336       EGF-like 8; calcium-binding (Potential).
DOMAIN      338    374       EGF-like 9; calcium-binding (Potential).
DOMAIN      375    413       EGF-like 10.
DOMAIN      415    454       EGF-like 11; calcium-binding (Potential).
DOMAIN      456    492       EGF-like 12; calcium-binding (Potential).
DOMAIN      494    530       EGF-like 13; calcium-binding (Potential).
DOMAIN      532    568       EGF-like 14; calcium-binding (Potential).
DOMAIN      570    605       EGF-like 15; calcium-binding (Potential).
DOMAIN      607    643       EGF-like 16; calcium-binding (Potential).
DOMAIN      645    680       EGF-like 17; calcium-binding (Potential).
DOMAIN      682    718       EGF-like 18; calcium-binding (Potential).
DOMAIN      720    755       EGF-like 19.
DOMAIN      757    793       EGF-like 20; calcium-binding (Potential).
DOMAIN      795    831       EGF-like 21; calcium-binding (Potential).
DOMAIN      833    871       EGF-like 22.
DOMAIN      873    909       EGF-like 23; calcium-binding (Potential).
DOMAIN      911    947       EGF-like 24; calcium-binding (Potential).
DOMAIN      949    985       EGF-like 25; calcium-binding (Potential).
DOMAIN      987   1023       EGF-like 26; calcium-binding (Potential).
DOMAIN     1025   1061       EGF-like 27; calcium-binding (Potential).
DOMAIN     1063   1099       EGF-like 28.
DOMAIN     1101   1147       EGF-like 29.
DOMAIN     1149   1185       EGF-like 30; calcium-binding (Potential).
DOMAIN     1187   1223       EGF-like 31; calcium-binding (Potential).
DOMAIN     1225   1262       EGF-like 32; calcium-binding (Potential).
DOMAIN     1264   1302       EGF-like 33.
DOMAIN     1304   1343       EGF-like 34.
DOMAIN     1374   1412       EGF-like 35.
REPEAT     1425   1465       LNR 1.
REPEAT     1466   1502       LNR 2.
REPEAT     1503   1544       LNR 3.
REPEAT     1827   1871       ANK 1.
REPEAT     1876   1905       ANK 2.
REPEAT     1909   1939       ANK 3.
REPEAT     1943   1972       ANK 4.
REPEAT     1976   2005       ANK 5.
REPEAT     2009   2038       ANK 6.
REGION     1425   1677       Negative regulatory region (NRR).
COMPBIAS   1645   1648       Poly-Ala.
COMPBIAS   1994   1997       Poly-Leu.
COMPBIAS   2426   2429       Poly-Ser.
MOD_RES    1716   1716       Phosphothreonine.
MOD_RES    1778   1778       Phosphoserine.
MOD_RES    1802   1802       Phosphothreonine.
MOD_RES    1804   1804       Phosphoserine.
MOD_RES    1808   1808       Phosphothreonine.
MOD_RES    1841   1841       Phosphoserine.
MOD_RES    1842   1842       Phosphoserine.
MOD_RES    1845   1845       Phosphoserine.
MOD_RES    2070   2070       Phosphoserine.
MOD_RES    2074   2074       Phosphothreonine.
MOD_RES    2078   2078       Phosphoserine (By similarity).
MOD_RES    2081   2081       Phosphoserine.
MOD_RES    2097   2097       Phosphothreonine.
CARBOHYD     46     46       N-linked (GlcNAc...) (Potential).
CARBOHYD    155    155       N-linked (GlcNAc...) (Potential).
CARBOHYD    733    733       N-linked (GlcNAc...) (Potential).
CARBOHYD   1102   1102       N-linked (GlcNAc...) (Potential).
CARBOHYD   1465   1465       N-linked (GlcNAc...) (Potential).
DISULFID     28     41       By similarity.
DISULFID     35     51       By similarity.
DISULFID     53     62       By similarity.
DISULFID     68     79       By similarity.
DISULFID     73     90       By similarity.
DISULFID     92    101       By similarity.
DISULFID    109    121       By similarity.
DISULFID    115    131       By similarity.
DISULFID    133    142       By similarity.
DISULFID    148    159       By similarity.
DISULFID    153    168       By similarity.
DISULFID    170    179       By similarity.
DISULFID    186    198       By similarity.
DISULFID    192    207       By similarity.
DISULFID    209    218       By similarity.
DISULFID    225    236       By similarity.
DISULFID    230    246       By similarity.
DISULFID    248    257       By similarity.
DISULFID    264    275       By similarity.
DISULFID    269    284       By similarity.
DISULFID    286    295       By similarity.
DISULFID    302    315       By similarity.
DISULFID    309    324       By similarity.
DISULFID    326    335       By similarity.
DISULFID    342    353       By similarity.
DISULFID    347    362       By similarity.
DISULFID    364    373       By similarity.
DISULFID    379    390       By similarity.
DISULFID    384    401       By similarity.
DISULFID    403    412       By similarity.
DISULFID    419    433       By similarity.
DISULFID    427    442       By similarity.
DISULFID    444    453       By similarity.
DISULFID    460    471       By similarity.
DISULFID    465    480       By similarity.
DISULFID    482    491       By similarity.
DISULFID    498    509       By similarity.
DISULFID    503    518       By similarity.
DISULFID    520    529       By similarity.
DISULFID    536    547       By similarity.
DISULFID    541    556       By similarity.
DISULFID    558    567       By similarity.
DISULFID    574    584       By similarity.
DISULFID    579    593       By similarity.
DISULFID    595    604       By similarity.
DISULFID    611    622       By similarity.
DISULFID    616    631       By similarity.
DISULFID    633    642       By similarity.
DISULFID    649    659       By similarity.
DISULFID    654    668       By similarity.
DISULFID    670    679       By similarity.
DISULFID    686    697       By similarity.
DISULFID    691    706       By similarity.
DISULFID    708    717       By similarity.
DISULFID    724    734       By similarity.
DISULFID    729    743       By similarity.
DISULFID    745    754       By similarity.
DISULFID    761    772       By similarity.
DISULFID    766    781       By similarity.
DISULFID    783    792       By similarity.
DISULFID    799    810       By similarity.
DISULFID    804    819       By similarity.
DISULFID    821    830       By similarity.
DISULFID    837    848       By similarity.
DISULFID    842    859       By similarity.
DISULFID    861    870       By similarity.
DISULFID    877    888       By similarity.
DISULFID    882    897       By similarity.
DISULFID    899    908       By similarity.
DISULFID    915    926       By similarity.
DISULFID    920    935       By similarity.
DISULFID    937    946       By similarity.
DISULFID    953    964       By similarity.
DISULFID    958    973       By similarity.
DISULFID    975    984       By similarity.
DISULFID    991   1002       By similarity.
DISULFID    996   1011       By similarity.
DISULFID   1013   1022       By similarity.
DISULFID   1029   1040       By similarity.
DISULFID   1034   1049       By similarity.
DISULFID   1051   1060       By similarity.
DISULFID   1067   1078       By similarity.
DISULFID   1072   1087       By similarity.
DISULFID   1089   1098       By similarity.
DISULFID   1105   1126       By similarity.
DISULFID   1120   1135       By similarity.
DISULFID   1137   1146       By similarity.
DISULFID   1153   1164       By similarity.
DISULFID   1158   1173       By similarity.
DISULFID   1175   1184       By similarity.
DISULFID   1191   1202       By similarity.
DISULFID   1196   1211       By similarity.
DISULFID   1213   1222       By similarity.
DISULFID   1229   1241       By similarity.
DISULFID   1235   1250       By similarity.
DISULFID   1252   1261       By similarity.
DISULFID   1268   1281       By similarity.
DISULFID   1273   1290       By similarity.
DISULFID   1292   1301       By similarity.
DISULFID   1308   1319       By similarity.
DISULFID   1313   1331       By similarity.
DISULFID   1333   1342       By similarity.
DISULFID   1378   1389       By similarity.
DISULFID   1383   1400       By similarity.
DISULFID   1402   1411       By similarity.
DISULFID   1425   1448
DISULFID   1430   1443
DISULFID   1439   1455
DISULFID   1466   1489
DISULFID   1472   1484
DISULFID   1480   1496
DISULFID   1503   1527
DISULFID   1509   1522
DISULFID   1518   1534
DISULFID   1632   1639
VARIANT     444    444       C -> Y (in ALGS2).
                             /FTId=VAR_029361.
VARIANT    1667   1667       V -> F (in dbSNP:rs17024517).
                             /FTId=VAR_031463.
CONFLICT     21     21       A -> T (in Ref. 2; AAG37073).
CONFLICT    210    210       P -> L (in Ref. 2; AAG37073).
CONFLICT   1037   1037       E -> D (in Ref. 4; AAB19224).
CONFLICT   1084   1085       ES -> SP (in Ref. 4; AAB19224).
CONFLICT   1094   1094       A -> V (in Ref. 4; AAB19224).
CONFLICT   1139   1139       L -> V (in Ref. 4; AAB19224).
CONFLICT   1519   1519       D -> N (in Ref. 1; AAA36377).
CONFLICT   2053   2053       R -> H (in Ref. 2; AAG37073).
HELIX      1428   1433
STRAND     1436   1438
HELIX      1441   1443
TURN       1446   1448
HELIX      1449   1452
TURN       1453   1458
TURN       1462   1465
HELIX      1472   1474
STRAND     1477   1479
HELIX      1482   1484
TURN       1487   1490
HELIX      1491   1494
HELIX      1506   1512
STRAND     1515   1517
HELIX      1520   1522
HELIX      1525   1532
STRAND     1544   1553
HELIX      1555   1560
HELIX      1562   1573
STRAND     1575   1579
STRAND     1589   1594
STRAND     1617   1628
HELIX      1632   1635
HELIX      1643   1656
STRAND     1663   1670
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Nucleotide Sequence
Length: 9722 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 2471 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Other Protein-Protein interaction resources
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