SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000525 
UniProt Accession
Theoretical PI
FRAGMENT  
Molecular Weight
54671 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
KLF4 
Gene Synonyms/Alias
EZF, GKLF 
Protein Name
Krueppel-like factor 4 
Protein Synonyms/Alias
Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:9;110247133-110252763;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
The strong expression of KLF4 in the human testis and the importance of KLF4 in several mouse tissues suggest a significant role for KLF4 in the human testis. A first hint at a role for KLF4 during spermiogenesis could be the altered subcellular localization of the protein during arrested spermiogenesis. 
The information of related literatures
1. R. Behr, C. Deller, M. Godmann, T. Muller, M. Bergmann, R. Ivell and K. Steger (2007) Kruppel-like factor 4 expression in normal and pathological human testes. Mol Hum Reprod 13(11): 815-20. 

Abstract
Kruppel-like factor 4 (KLF4) is a transcription factor involved in many cellular and developmental processes such as terminal differentiation of cells and carcinogenesis. Mice lacking KLF4 die post-natally due to skin barrier deficiencies and exhibit several additional cellular defects. The adult rodent testis expresses high levels of Klf4 mRNA. Using in situ hybridization, we previously localized most of the Klf4 mRNA to round spermatids in mice. Moreover, in rodent Sertoli cells, Klf4 is strongly inducible by FSH. Here, we show by northern blot analysis that the human testis also strongly expresses KLF4. Applying immunohistochemistry, we localized KLF4 protein to the nuclei of round spermatids during normal spermatogenesis stages II-IV. Analysing round spermatid maturation arrests, strong cytoplasmic staining could be seen in two samples. We failed to detect KLF4 in human Sertoli cells. Most human Leydig cells expressed KLF4 at high levels in the nucleus. However, some individual Leydig cells lacked KLF4, suggesting different functional states of the Leydig cells. The strong expression of KLF4 in the human testis and the importance of KLF4 in several mouse tissues suggest a significant role for KLF4 in the human testis. A first hint at a role for KLF4 during spermiogenesis could be the altered subcellular localization of the protein during arrested spermiogenesis. PMID: [17932114] 

Back to Top
Figures for illustrating the function of this protein/gene
Ref: R. Behr, C. Deller, M. Godmann, T. Muller, M. Bergmann, R. Ivell and K. Steger (2007) Kruppel-like factor 4 expression in normal and pathological human testes. Mol Hum Reprod 13(11): 815-20. PMID: [17932114]
Ref: R. Behr, C. Deller, M. Godmann, T. Muller, M. Bergmann, R. Ivell and K. Steger (2007) Kruppel-like factor 4 expression in normal and pathological human testes. Mol Hum Reprod 13(11): 815-20. PMID: [17932114]
Function
Transcription factor; can act both as activator and asrepressor. Binds the 5'-CACCC-3' core sequence. Binds to thepromoter region of its own gene and can activate its owntranscription. Regulates the expression of key transcriptionfactors during embryonic development. Plays an important role inmaintaining embryonic stem cells, and in preventing theirdifferentiation. Required for establishing the barrier function ofthe skin and for postnatal maturation and maintenance of theocular surface. Involved in the differentiation of epithelialcells and may also function in skeletal and kidney development.Contributes to the down-regulation of p53/TP53 transcription. 
Back to Top
Subcellular Location
Nucleus. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0000790 C:nuclear chromatin IDA:BHF-UCL.
GO:0005667 C:transcription factor complex IEA:Compara.
GO:0000987 F:core promoter proximal region sequence-specific DNA binding IDA:BHF-UCL.
GO:0003690 F:double-stranded DNA binding IEA:Compara.
GO:0035014 F:phosphatidylinositol 3-kinase regulator activity IEA:Compara.
GO:0001077 F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription IMP:BHF-UCL.
GO:0001190 F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription IDA:BHF-UCL.
GO:0001010 F:sequence-specific DNA binding transcription factor recruiting transcription factor activity ISS:BHF-UCL.
GO:0008270 F:zinc ion binding NAS:UniProtKB.
GO:0071363 P:cellular response to growth factor stimulus IDA:BHF-UCL.
GO:0009913 P:epidermal cell differentiation IEA:Compara.
GO:0048730 P:epidermis morphogenesis IEA:Compara.
GO:0045444 P:fat cell differentiation ISS:BHF-UCL.
GO:0007500 P:mesodermal cell fate determination TAS:ProtInc.
GO:0090051 P:negative regulation of cell migration involved in sprouting angiogenesis IDA:BHF-UCL.
GO:0008285 P:negative regulation of cell proliferation TAS:ProtInc.
GO:2000342 P:negative regulation of chemokine (C-X-C motif) ligand 2 production IDA:BHF-UCL.
GO:0043154 P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process IDA:BHF-UCL.
GO:0070373 P:negative regulation of ERK1 and ERK2 cascade IEA:Compara.
GO:0034115 P:negative regulation of heterotypic cell-cell adhesion IDA:BHF-UCL.
GO:0050728 P:negative regulation of inflammatory response TAS:BHF-UCL.
GO:0045415 P:negative regulation of interleukin-8 biosynthetic process IDA:BHF-UCL.
GO:0014740 P:negative regulation of muscle hyperplasia IEA:Compara.
GO:0032088 P:negative regulation of NF-kappaB transcription factor activity IDA:BHF-UCL.
GO:0014067 P:negative regulation of phosphatidylinositol 3-kinase cascade IEA:Compara.
GO:0051898 P:negative regulation of protein kinase B signaling cascade IEA:Compara.
GO:0060761 P:negative regulation of response to cytokine stimulus IDA:BHF-UCL.
GO:0048662 P:negative regulation of smooth muscle cell proliferation IEA:Compara.
GO:0000122 P:negative regulation of transcription from RNA polymerase II promoter IDA:BHF-UCL.
GO:0046985 P:positive regulation of hemoglobin biosynthetic process IMP:BHF-UCL.
GO:0031077 P:post-embryonic camera-type eye development IEA:Compara.
GO:0035166 P:post-embryonic hemopoiesis IMP:BHF-UCL.
GO:0043551 P:regulation of phosphatidylinositol 3-kinase activity IEA:GOC.
GO:0032526 P:response to retinoic acid IEA:Compara.
GO:0019827 P:stem cell maintenance ISS:UniProtKB.
Back to Top
Interpro
IPR007087;    Znf_C2H2.
IPR015880;    Znf_C2H2-like.
IPR013087;    Znf_C2H2/integrase_DNA-bd.
Back to Top
Pfam
SMART
SM00355;    ZnF_C2H2;    3.
Back to Top
PROSITE
PS00028;    ZINC_FINGER_C2H2_1;    3.
PS50157;    ZINC_FINGER_C2H2_2;    3.
Back to Top
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    513       Krueppel-like factor 4.
                             /FTId=PRO_0000047167.
ZN_FING     430    454       C2H2-type 1.
ZN_FING     460    484       C2H2-type 2.
ZN_FING     490    512       C2H2-type 3.
REGION      473    504       Interaction with target DNA (By
                             similarity).
MOTIF       101    109       9aaTAD.
COMPBIAS    125    149       Ser-rich.
COMPBIAS    179    415       Pro-rich.
MOD_RES     254    254       Phosphoserine (By similarity).
CROSSLNK     32     32       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin).
VAR_SEQ       1     50       Missing (in isoform 3).
                             /FTId=VSP_040569.
VAR_SEQ     367    400       Missing (in isoform 2 and isoform 3).
                             /FTId=VSP_036399.
VARIANT     315    315       T -> S (in dbSNP:rs1059913).
                             /FTId=VAR_059888.
VARIANT     321    321       L -> F (in dbSNP:rs1059914).
                             /FTId=VAR_059889.
CONFLICT     60     61       GR -> AG (in Ref. 1; AAC03462).
CONFLICT     77     77       G -> A (in Ref. 1; AAC03462).
CONFLICT    251    251       S -> T (in Ref. 1; AAC03462).
CONFLICT    304    304       D -> N (in Ref. 3; AAB48399).
CONFLICT    329    329       D -> E (in Ref. 3; AAB48399).
Back to Top
Nucleotide Sequence
Length: 1876 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 513 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
Comments