SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000548 
UniProt Accession
Theoretical PI
5.35  
Molecular Weight
61055 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
HSPD1 
Gene Synonyms/Alias
HSP60 
Protein Name
60 kDa heat shock protein, mitochondrial 
Protein Synonyms/Alias
60 kDa chaperonin; Chaperonin 60;CPN60 Heat shock protein 60;HSP-60Hsp60 HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein;Flags: Precursor 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:2;198351305-198381461;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
These results indicate that in addition to being expressed in human testis spermatogenic cells, both Hsp60 and Grp78 proteins persist in ejaculated spermatozoa. These findings are in agreement with the involvement of Hsp60 and Grp78 during spermatogenesis and in sperm functions such as fertilization. 
The information of related literatures
1. C. Lachance, M. Fortier, V. Thimon, R. Sullivan, J. L. Bailey and P. Leclerc (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl 33(1): 33-44. 

Abstract
Molecular chaperones of the heat shock proteins (HSP) family are important in numerous cellular processes. In this study, the expression of Hsp60 and Grp78 proteins was investigated in the male reproductive tract. The cellular distribution of Hsp60 and Grp78 proteins was analysed in the human testis and epididymis by immunohistochemical approaches. DNA microarray technology was used to analyse HSP60 and GRP78 gene expression along human epididymis. The cellular localization of these chaperone proteins in ejaculated spermatozoa was investigated by indirect immunofluorescence and by Western blot following sperm sub-cellular fractionation. In the human testis, Hsp60 was detected in spermatogonia, whereas a strong Grp78 staining was observed in spermatocytes and round spermatids. Grp78 protein was also observed in the epididymal epithelium, whereas no Hsp60 staining was observed in this organ by immunohistochemistry. The presence of both Hsp60 and Grp78 RNA in human epididymis was confirmed by microarrays. In ejaculated spermatozoa, Hsp60 was localized in the mid-piece, whereas Grp78 was detected in the neck region. These results indicate that in addition to being expressed in human testis spermatogenic cells, both Hsp60 and Grp78 proteins persist in ejaculated spermatozoa. These findings are in agreement with the involvement of Hsp60 and Grp78 during spermatogenesis and in sperm functions such as fertilization. PMID: [19207617] 

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Figures for illustrating the function of this protein/gene
Ref: C. Lachance, M. Fortier, V. Thimon, R. Sullivan, J. L. Bailey and P. Leclerc (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl 33(1): 33-44. PMID: [19207617]
Ref: C. Lachance, M. Fortier, V. Thimon, R. Sullivan, J. L. Bailey and P. Leclerc (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl 33(1): 33-44. PMID: [19207617]
Ref: C. Lachance, M. Fortier, V. Thimon, R. Sullivan, J. L. Bailey and P. Leclerc (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl 33(1): 33-44. PMID: [19207617]
Function
Implicated in mitochondrial protein import andmacromolecular assembly. May facilitate the correct folding ofimported proteins. May also prevent misfolding and promote therefolding and proper assembly of unfolded polypeptides generatedunder stress conditions in the mitochondrial matrix. 
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Subcellular Location
Mitochondrion matrix. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0009986 C:cell surface IDA:UniProtKB.
GO:0005905 C:coated pit IDA:BHF-UCL.
GO:0030135 C:coated vesicle IDA:BHF-UCL.
GO:0005829 C:cytosol IDA:UniProtKB.
GO:0005769 C:early endosome IDA:BHF-UCL.
GO:0005615 C:extracellular space IDA:BHF-UCL.
GO:0046696 C:lipopolysaccharide receptor complex IDA:BHF-UCL.
GO:0005743 C:mitochondrial inner membrane ISS:BHF-UCL.
GO:0005759 C:mitochondrial matrix TAS:BHF-UCL.
GO:0044459 C:plasma membrane part IEA:Compara.
GO:0030141 C:secretory granule ISS:BHF-UCL.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0016887 F:ATPase activity ISS:BHF-UCL.
GO:0043498 F:cell surface binding IDA:BHF-UCL.
GO:0003688 F:DNA replication origin binding ISS:BHF-UCL.
GO:0001530 F:lipopolysaccharide binding IDA:BHF-UCL.
GO:0003697 F:single-stranded DNA binding ISS:BHF-UCL.
GO:0006458 P:'de novo' protein folding ISS:BHF-UCL.
GO:0006919 P:activation of cysteine-type endopeptidase activity involved in apoptotic process IDA:BHF-UCL.
GO:0002368 P:B cell cytokine production IDA:BHF-UCL.
GO:0042100 P:B cell proliferation IDA:BHF-UCL.
GO:0051131 P:chaperone-mediated protein complex assembly ISS:BHF-UCL.
GO:0048291 P:isotype switching to IgG isotypes IDA:BHF-UCL.
GO:0002755 P:MyD88-dependent toll-like receptor signaling pathway IDA:BHF-UCL.
GO:0043066 P:negative regulation of apoptotic process IMP:UniProtKB.
GO:0043065 P:positive regulation of apoptotic process IMP:BHF-UCL.
GO:0032727 P:positive regulation of interferon-alpha production IDA:BHF-UCL.
GO:0032729 P:positive regulation of interferon-gamma production IDA:BHF-UCL.
GO:0032733 P:positive regulation of interleukin-10 production IDA:BHF-UCL.
GO:0032735 P:positive regulation of interleukin-12 production IDA:BHF-UCL.
GO:0032755 P:positive regulation of interleukin-6 production IDA:BHF-UCL.
GO:0043032 P:positive regulation of macrophage activation IDA:BHF-UCL.
GO:0050870 P:positive regulation of T cell activation IDA:BHF-UCL.
GO:0002842 P:positive regulation of T cell mediated immune response to tumor cell IDA:BHF-UCL.
GO:0051604 P:protein maturation ISS:BHF-UCL.
GO:0042026 P:protein refolding IDA:UniProtKB.
GO:0050821 P:protein stabilization IMP:UniProtKB.
GO:0006986 P:response to unfolded protein IDA:BHF-UCL.
GO:0042110 P:T cell activation IDA:MGI.
GO:0019048 P:virus-host interaction IEA:UniProtKB-KW.
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Interpro
IPR018370;    Chaperonin_Cpn60_CS.
IPR001844;    Chaprnin_Cpn60.
IPR002423;    Cpn60/TCP-1.
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Pfam
PF00118;    Cpn60_TCP1;    1.
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SMART
PROSITE
PS00296;    CHAPERONINS_CPN60;    1.
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PRINTS
PR00298;    CHAPERONIN60.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
TRANSIT       1     26       Mitochondrion.
CHAIN        27    573       60 kDa heat shock protein, mitochondrial.
                             /FTId=PRO_0000005026.
MOD_RES      31     31       N6-acetyllysine.
MOD_RES      70     70       Phosphoserine.
MOD_RES      82     82       N6-acetyllysine.
MOD_RES     125    125       N6-acetyllysine.
MOD_RES     130    130       N6-acetyllysine.
MOD_RES     133    133       N6-malonyllysine.
MOD_RES     202    202       N6-acetyllysine.
MOD_RES     218    218       N6-acetyllysine.
MOD_RES     223    223       Phosphotyrosine (By similarity).
MOD_RES     227    227       Phosphotyrosine.
MOD_RES     269    269       N6-acetyllysine.
MOD_RES     352    352       N6-acetyllysine.
MOD_RES     359    359       N6-acetyllysine.
MOD_RES     396    396       N6-acetyllysine.
MOD_RES     469    469       N6-acetyllysine.
MOD_RES     473    473       N6-acetyllysine.
MOD_RES     523    523       N6-acetyllysine.
VARIANT      29     29       D -> G (in HLD4; transfection with the
                             mutant protein impairs cell growth that
                             worsens with increasing temperature).
                             /FTId=VAR_054785.
VARIANT      98     98       V -> I (in SPG13).
                             /FTId=VAR_026748.
CONFLICT     67     67       S -> G (in Ref. 2; AAA36022).
CONFLICT    111    111       D -> N (in Ref. 5; BAG35173).
CONFLICT    177    177       N -> S (in Ref. 5; BAG35173).
CONFLICT    202    202       K -> KAS (in Ref. 4; ABB01006).
CONFLICT    260    260       A -> T (in Ref. 5; BAG35173).
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Nucleotide Sequence
Length: 2227 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 573 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
YWHAGHPRD 
YWHAZMINT 
PPP2R1AHPRD 
PPP2R1BHPRD 
PPP2R2BIntAct 
PPP2R1AHPRD 
ACTBIntAct 
EIF2C1DIP 
EIF2C2DIP 
ALDH2BioGRID 
ALKBioGRID 
AMFRBioGRID 
DYRHPRD 
ALKBioGRID 
BAK1HPRD 
CDH1IntAct 
CA2HPRD 
CASP3HPRD 
CASP9HPRD 
CDK9BioGRID 
CFTRIntAct 
HSPE1HPRD 
FDXRIntAct 
ALDH2HPRD 
ALKBioGRID 
ALKBioGRID 
BAK1BioGRID 
BAXBioGRID 
CASP3BioGRID 
HSPE1BioGRID 
HSPD1HPRD 
HSPD1HPRD 
ALDH2BioGRID 
FHITIntAct 
HSPA5IntAct 
hemL2IntAct 
HIST2H2BEBioGRID 
HSPA1ADIP 
iolA2IntAct 
ITGA3HPRD 
IKBKGIntAct 
TLR1HPRD 
BAS3164IntAct 
RAF1IntAct 
SMAD2IntAct 
MPSTDIP 
TLR1HPRD 
DHFRHPRD 
NR3C1BioGRID 
NR3C1BioGRID 
ALDH2BioGRID 
EBNA-LPMINT 
EGFRIntAct 
ESR1IntAct 
NR3C1BioGRID 
FHITHPRD 
GABARAPIntAct 
GABARAPL1IntAct 
NR3C1BioGRID 
GLULDIP 
GRB2IntAct 
HIST2H2BEHPRD 
HDAC1BioGRID 
HNRNPH1BioGRID 
HSP90AA1DIP 
PRKACAHPRD 
Mcl1MINT 
MED19IntAct 
MED9IntAct 
MAPK6BioGRID 
MAP1LC3BIntAct 
MYCIntAct 
IKBKGMINT 
PRNPHPRD 
PRNPDIP 
SF3A2BioGRID 
FHITHPRD 
PRNPHPRD 
_HPRD 
GABARAPL2IntAct 
HDAC1BioGRID 
YPO4108IntAct 
YPO0388IntAct 
RAD52IntAct 
RASA1HPRD 
RPAP1BioGRID 
SF3A2BioGRID 
SIRT1IntAct 
SIRT3IntAct 
SPG20IntAct 
SRRM2BioGRID 
SUMO4HPRD 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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