SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000550 
UniProt Accession
Theoretical PI
5.62  
Molecular Weight
29203 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
CTDSP1 
Gene Synonyms/Alias
NIF3, NLIIF, SCP1 
Protein Name
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 
Protein Synonyms/Alias
EC=3.1.3.16 Nuclear LIM interactor-interacting factor 3;NLI-IFNLI-interacting factor 3 Small C-terminal domain phosphatase 1;SCP1Small CTD phosphatase 1 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:2;219262979-219270664;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
We found that antibodies raised against a protein component (P1) of the mouse synaptonemal complex, mouse SCP1, also identified the SC in human primary spermatocytes.  
The information of related literatures
1. A. Pousette, P. Leijonhufvud, S. Arver, U. Kvist, J. Pelttari and C. Hoog (1997) Presence of synaptonemal complex protein 1 transversal filament-like protein in human primary spermatocytes. Hum Reprod 12(11): 2414-7. 

Abstract
The synaptonemal complex (SC) is involved in the pairing of chromosomes during meiosis. We found that antibodies raised against a protein component (P1) of the mouse synaptonemal complex, mouse SCP1, also identified the SC in human primary spermatocytes. Biopsies from 18 men presented with infertility were evaluated by light-field microscopy and grouped into five categories PMID: [9436675] 

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Figures for illustrating the function of this protein/gene
Ref: A. Pousette, P. Leijonhufvud, S. Arver, U. Kvist, J. Pelttari and C. Hoog (1997) Presence of synaptonemal complex protein 1 transversal filament-like protein in human primary spermatocytes. Hum Reprod 12(11): 2414-7. PMID: [9436675]
Ref: A. Pousette, P. Leijonhufvud, S. Arver, U. Kvist, J. Pelttari and C. Hoog (1997) Presence of synaptonemal complex protein 1 transversal filament-like protein in human primary spermatocytes. Hum Reprod 12(11): 2414-7. PMID: [9436675]
Function
Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain(CTD) of the largest RNA polymerase II subunit POLR2A. Negativelyregulates RNA polymerase II transcription, possibly by controllingthe transition from initiation/capping to processive transcriptelongation. Recruited by REST to neuronal genes that contain RE-1elements, leading to neuronal gene silencing in non-neuronalcells. 
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Subcellular Location
Nucleus. Note=Colocalizes with RNApolymerase II. 
Tissue Specificity
Expression is restricted to non-neuronaltissues. Highest expression in skeletal muscle, spleen, lung andplacenta. 
Gene Ontology
GO IDGO termEvidence
GO:0005634 C:nucleus IDA:UniProtKB.
GO:0008420 F:CTD phosphatase activity IDA:UniProtKB.
GO:0046872 F:metal ion binding IEA:UniProtKB-KW.
GO:0006470 P:protein dephosphorylation IDA:UniProtKB.
GO:0006357 P:regulation of transcription from RNA polymerase II promoter IDA:UniProtKB.
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Interpro
IPR011948;    Dullard_phosphatase.
IPR023214;    HAD-like_dom.
IPR004274;    NIF.
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Pfam
PF03031;    NIF;    1.
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SMART
SM00577;    CPDc;    1.
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PROSITE
PS50969;    FCP1;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    261       Carboxy-terminal domain RNA polymerase II
                             polypeptide A small phosphatase 1.
                             /FTId=PRO_0000212572.
DOMAIN       86    244       FCP1 homology.
ACT_SITE     96     96       4-aspartylphosphate intermediate.
ACT_SITE     98     98       Proton donor.
METAL        96     96       Magnesium.
METAL        98     98       Magnesium; via carbonyl oxygen.
METAL       207    207       Magnesium.
SITE        152    152       Transition state stabilizer.
SITE        190    190       Transition state stabilizer.
VAR_SEQ       1     48       Missing (in isoform 2).
                             /FTId=VSP_038541.
VAR_SEQ      49     72       DDGEALPAHSGAPLLVEENGAIPK -> MMGRPCLLTAGRP
                             CLWRRMAPSLR (in isoform 2).
                             /FTId=VSP_038542.
VARIANT      56     56       A -> T (in dbSNP:rs2227249).
                             /FTId=VAR_049054.
MUTAGEN      96     96       D->E: No effect. Completely abolishes
                             phosphatase activity; when associated
                             with N-98.
MUTAGEN      98     98       D->N: Completely abolishes phosphatase
                             activity; when associated with E-96.
CONFLICT     73     73       Missing (in Ref. 2; AAP34397).
HELIX        85     87
STRAND       92     95
TURN         99    101
STRAND      102    107
STRAND      113    120
STRAND      123    131
HELIX       135    145
STRAND      146    151
HELIX       156    166
STRAND      172    176
HELIX       178    180
STRAND      181    184
STRAND      187    189
HELIX       192    194
STRAND      195    197
HELIX       199    201
STRAND      202    205
HELIX       209    212
HELIX       216    218
STRAND      219    221
HELIX       233    244
HELIX       251    254
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Nucleotide Sequence
Length: 32198 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 261 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
CDCA3IntAct 
_HPRD 
CDCA3HPRD 
MBPHPRD 
POLR2ABioGRID 
SNAI1HPRD 
GTF2F1HPRD 
LDB1HPRD 
POLR2AHPRD 
SNAI1BioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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