SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000558 
UniProt Accession
Theoretical PI
5.96  
Molecular Weight
157916 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
CCNB3 
Gene Synonyms/Alias
CYCB3 
Protein Name
G2/mitotic-specific cyclin-B3 
Protein Synonyms/Alias
 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:X;49967364-50094909;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
The expression pattern of mammalian cyclin B3 suggests that it may be important for events occurring in early meiotic prophase I. 
The information of related literatures
1. T. B. Nguyen, K. Manova, P. Capodieci, C. Lindon, S. Bottega, X. Y. Wang, J. Refik-Rogers, J. Pines, D. J. Wolgemuth and A. Koff (2002) Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin. J Biol Chem 277(44): 41960-9. 

Abstract
We report the identification and expression pattern of a full-length human cDNA and a partial mouse cDNA encoding cyclin B3. Cyclin B3 (CCNB3) is conserved from Caenorhabditis elegans to Homo sapiens and has an undefined meiotic function in female, but not male Drosophila melanogaster. We show that H. sapiens cyclin B3 interacts with cdk2, is localized to the nucleus, and is degraded during anaphase entry after the degradation of cyclin B1. Degradation is dependent on sequences conserved in a destruction box motif. Overexpression of nondegradable cyclin B3 blocks the mitotic cell cycle in late anaphase, and at higher doses it can interfere with progression through G(1) and entry into S phase. H. sapiens cyclin B3 mRNA and protein are detected readily in developing germ cells in the human testis and not in any other tissue. The mouse cDNA has allowed us to further localize cyclin B3 mRNA to leptotene and zygotene spermatocytes. The expression pattern of mammalian cyclin B3 suggests that it may be important for events occurring in early meiotic prophase I. PMID: [12185076] 

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Figures for illustrating the function of this protein/gene
Ref: T. B. Nguyen, K. Manova, P. Capodieci, C. Lindon, S. Bottega, X. Y. Wang, J. Refik-Rogers, J. Pines, D. J. Wolgemuth and A. Koff (2002) Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin. J Biol Chem 277(44): 41960-9. PMID: [12185076]
Ref: T. B. Nguyen, K. Manova, P. Capodieci, C. Lindon, S. Bottega, X. Y. Wang, J. Refik-Rogers, J. Pines, D. J. Wolgemuth and A. Koff (2002) Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin. J Biol Chem 277(44): 41960-9. PMID: [12185076]
Ref: T. B. Nguyen, K. Manova, P. Capodieci, C. Lindon, S. Bottega, X. Y. Wang, J. Refik-Rogers, J. Pines, D. J. Wolgemuth and A. Koff (2002) Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin. J Biol Chem 277(44): 41960-9. PMID: [12185076]
Ref: T. B. Nguyen, K. Manova, P. Capodieci, C. Lindon, S. Bottega, X. Y. Wang, J. Refik-Rogers, J. Pines, D. J. Wolgemuth and A. Koff (2002) Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin. J Biol Chem 277(44): 41960-9. PMID: [12185076]
Ref: T. B. Nguyen, K. Manova, P. Capodieci, C. Lindon, S. Bottega, X. Y. Wang, J. Refik-Rogers, J. Pines, D. J. Wolgemuth and A. Koff (2002) Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin. J Biol Chem 277(44): 41960-9. PMID: [12185076]
Function
Cyclins are positive regulatory subunits of the cyclin-dependent kinases (CDKs), and thereby play an essential role inthe control of the cell cycle, notably via their destructionduring cell division. Its tissue specificity suggest that it maybe required during early meiotic prophase I. 
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Subcellular Location
Nucleus. 
Tissue Specificity
Testis specific. In testis, it is expressed indeveloping germ cells, but not in Leydig cells. Weakly or notexpressed in other tissues. 
Gene Ontology
GO IDGO termEvidence
GO:0005634 C:nucleus IDA:HPA.
GO:0051301 P:cell division IEA:UniProtKB-KW.
GO:0007126 P:meiosis IEA:UniProtKB-KW.
GO:0000079 P:regulation of cyclin-dependent protein kinase activity IEA:InterPro.
GO:0010389 P:regulation of G2/M transition of mitotic cell cycle IEA:InterPro.
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Interpro
IPR013763;    Cyclin-like.
IPR015452;    Cyclin_B3.
IPR004367;    Cyclin_C.
IPR006671;    Cyclin_N.
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Pfam
PF02984;    Cyclin_C;    1.
PF00134;    Cyclin_N;    1.
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SMART
SM00385;    CYCLIN;    2.
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PROSITE
PS00292;    CYCLINS;    FALSE_NEG.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1395       G2/mitotic-specific cyclin-B3.
                             /FTId=PRO_0000080373.
MOTIF        60     68       D-box.
MOD_RES    1038   1038       Phosphothreonine.
VAR_SEQ      69   1172       Missing (in isoform 2).
                             /FTId=VSP_010514.
VAR_SEQ     112   1395       Missing (in isoform 3).
                             /FTId=VSP_010515.
VARIANT     597    597       K -> T (in a colorectal cancer sample;
                             somatic mutation).
                             /FTId=VAR_036580.
VARIANT    1001   1001       G -> R (in dbSNP:rs6614336).
                             /FTId=VAR_047027.
MUTAGEN      60     60       R->A: In cycB3XA; prevents its
                             destruction after completion of anaphase;
                             when associated with A-63 and A-68.
MUTAGEN      63     63       F->A: In cycB3XA; prevents its
                             destruction after completion of anaphase;
                             when associated with A-60 and A-68.
MUTAGEN      68     68       N->A: In cycB3XA; prevents its
                             destruction after completion of anaphase;
                             when associated with A-60 and A-63.
CONFLICT    295    295       K -> R (in Ref. 2; CAC40024).
CONFLICT    331    331       S -> F (in Ref. 1; CAC94915).
CONFLICT    396    396       S -> P (in Ref. 2; CAC40024).
CONFLICT    493    493       R -> K (in Ref. 2; CAC40024).
CONFLICT    573    573       N -> S (in Ref. 2; CAC40024).
CONFLICT    597    597       K -> E (in Ref. 2; CAC40024).
CONFLICT    774    774       E -> K (in Ref. 1; CAC94915).
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Nucleotide Sequence
Length: 4540 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1395 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
CDK2IntAct 
CDK2HPRD 
CDK2deltaTBioGRID 
Plk4MINT 
Other Protein-Protein interaction resources
String database  
View Microarray data
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