SG ID

SG00000561 

UniProt Accession

SPT25_HUMAN;Q9BR10;  

Theoretical PI

8.59  

Molecular Weight

23834 Da  

Genbank Nucleotide ID

AL008726; BC039607;  

Genbank Protein ID

CAC36017.1; AAH39607.1; 

Gene Name

SPATA25 

Gene Synonyms/Alias

C20orf165, TSG23 

Protein Name

Spermatogenesis-associated protein 25 

Protein Synonyms/Alias

Testis-specific gene 23 protein; 

Homo sapiens (Human) 

9606 

The expression of Tsg23 mRNA was considerably decreased in a time-dependent manner in the testis of an azoospermic mouse model induced by Busulfan. These data suggest that TSG23/Tsg23 is involved in human and mouse spermatogenesis. 

The information of related literatures

1. Y. Zhou, D. Qin, A. Tang, D. Zhou, J. Qin, B. Yan, R. Diao, Z. Jiang, Z. Cai and Y. Gui (2009) Developmental expression pattern of a novel gene, TSG23/Tsg23, suggests a role in spermatogenesis. Mol Hum Reprod 15(4): 223-30. 

Abstract
A novel gene, TSG23/Tsg23, was identified by comparing the expression profiles of human adult and fetal testis using Affymetrix Genechips. RT-PCR analysis from multiple human and mouse tissues indicated TSG23/Tsg23 mRNA was mainly expressed in the testis. In situ hybridization revealed that TSG23/Tsg23 mRNA was located in spermatocytes and round spermatids of the seminiferous tubules in human and mouse testis. To further confirm the result from RT-PCR, the antibody for human TSG23 was generated against the protein encoded by the gene. Western blot analysis demonstrated that TSG23 was mainly expressed in human testis, with a molecular weight of about 23 kDa. Immunohistochemistry showed that TSG23 was predominantly located in spermatocytes and round spermatids, consistent with the results from in situ hybridization. In order to explore the function of TSG23 in spermatogenesis, the study compared the expression of TSG23 in the testis from fertile persons and from patients with azoospermia. The results showed that there was less expression in patients with obstructive azoospermia compared with fertile persons, and no detectable TSG23 at mRNA and protein levels in patients with non-obstructive azoospermia. The expression of Tsg23 mRNA was considerably decreased in a time-dependent manner in the testis of an azoospermic mouse model induced by Busulfan. These data suggest that TSG23/Tsg23 is involved in human and mouse spermatogenesis. PMID: [19240080] 

Figures for illustrating the function of this protein/gene

Function

May play a role in spermatogenesis. 

Subcellular Location

Membrane; Single-pass membrane protein(Potential). 

Tissue Specificity

Expressed predominantly in testis (at proteinlevel). Expression is lower in patients with obstructiveazoospermia than in fertile controls and is not detected inpatients with non-obstructive azoospermia. 

Gene Ontology

GO ID	GO term	Evidence
GO:0016021	C:integral to membrane	IEA:UniProtKB-KW.
GO:0030154	P:cell differentiation	IEA:UniProtKB-KW.
GO:0007283	P:spermatogenesis	IEP:UniProtKB.

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 86080 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 227 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

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