SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000595 
UniProt Accession
Theoretical PI
9.21  
Molecular Weight
43081 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
SUN5 
Gene Synonyms/Alias
SPAG4L, TSARG4 
Protein Name
SUN domain-containing protein 5 
Protein Synonyms/Alias
Sad1 and UNC84 domain-containing protein 5; Sperm-associated antigen 4-like protein; Testis and spermatogenesis-related gene 4 protein; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:20;31571579-31592239;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Together, the findings indicate that SPAG4L, a new NE protein, may play an important role in the meiotic stage of spermatogenesis. 
The information of related literatures
1. X. Z. Jiang, M. G. Yang, L. H. Huang, C. Q. Li and X. W. Xing (2011) SPAG4L, a Novel Nuclear Envelope Protein Involved in the Meiotic Stage of Spermatogenesis. DNA Cell Biol (): . 

Abstract
SUN domain-containing proteins belong to a novel protein family. To date, several members-SUN1, SUN2, SUN3, and SPAG4-have been identified as nuclear envelope (NE) proteins. In this study, we sought to characterize and define the potential function of SPAG4L, a newly identified SUN protein. Using bioinformatic analysis, we found that SPAG4L contained a conserved SUN domain in the C-terminal. Subcellular localization analysis indicated that the expression of green fluorescent protein-labeled full-length SPAG4L was localized to the NE and the endoplasmic reticulum (ER). Deletion analysis revealed that the transmembrane region and the coiled-coil domain, but not the SUN domain, were required for localization of SPAG4L to the NE and ER. Subsequently, we confirmed that the human testes expressed endogenous SPAG4L as a 43-kDa protein. Further studies revealed that mouse Spag4L colocalized with the NE marker Lamin B1 and the ER marker PDI in isolated mouse spermatocytes. In addition, the expression of Spag4L was observed in meiosis I and II stages, suggesting that Spag4L may be involved in NE reconstitution and nuclear migration occurring during the process of spermatocyte division. Together, the findings indicate that SPAG4L, a new NE protein, may play an important role in the meiotic stage of spermatogenesis. PMID: [21711156] 

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Figures for illustrating the function of this protein/gene
Ref: X. Z. Jiang, M. G. Yang, L. H. Huang, C. Q. Li and X. W. Xing (2011) SPAG4L, a Novel Nuclear Envelope Protein Involved in the Meiotic Stage of Spermatogenesis. DNA Cell Biol (): . PMID: [21711156]
Ref: X. Z. Jiang, M. G. Yang, L. H. Huang, C. Q. Li and X. W. Xing (2011) SPAG4L, a Novel Nuclear Envelope Protein Involved in the Meiotic Stage of Spermatogenesis. DNA Cell Biol (): . PMID: [21711156]
Ref: X. Z. Jiang, M. G. Yang, L. H. Huang, C. Q. Li and X. W. Xing (2011) SPAG4L, a Novel Nuclear Envelope Protein Involved in the Meiotic Stage of Spermatogenesis. DNA Cell Biol (): . PMID: [21711156]
Function
 
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Subcellular Location
 
Tissue Specificity
Widely expressed. 
Gene Ontology
GO IDGO termEvidence
GO:0007283 P:spermatogenesis IEP:UniProtKB.
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Interpro
IPR012919;    Sad1_UNC_C.
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Pfam
PF07738;    Sad1_UNC;    1.
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SMART
PROSITE
PS51469;    SUN;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    379       SUN domain-containing protein 5.
                             /FTId=PRO_0000218919.
DOMAIN      205    364       SUN.
COILED      141    182       Potential.
VARIANT      16     16       E -> K (in dbSNP:rs3746387).
                             /FTId=VAR_015147.
VARIANT      39     39       E -> D (in dbSNP:rs1133358).
                             /FTId=VAR_026677.
VARIANT     120    120       I -> V (in dbSNP:rs35216976).
                             /FTId=VAR_052285.
VARIANT     174    174       A -> T (in dbSNP:rs17123951).
                             /FTId=VAR_026678.
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Nucleotide Sequence
Length: 1252 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 379 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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