SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000615 
UniProt Accession
Theoretical PI
5.06  
Molecular Weight
31540 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
CALB2 
Gene Synonyms/Alias
CAB29 
Protein Name
Calretinin 
Protein Synonyms/Alias
CR 29 kDa calbindin; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:16;71392616-71424341;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
This pattern of calretinin-selective expression in immature Sertoli cells suggests a functional relationship between calretinin expression and the degree of Sertoli cell differentiation. Disorders of Sertoli cell differentiation as indicated by calretinin and/or CK-18 expression contribute to the multifactorial mechanisms underlying spermatogenic fai. 
The information of related literatures
1. B. Bar-Shira Maymon, H. Yavetz, L. Yogev, S. E. Kleiman, B. Lifschitz-Mercer, L. Schreiber, A. Botchan, R. Hauser and G. Paz (2005) Detection of calretinin expression in abnormal immature Sertoli cells in non-obstructive azoospermia. Acta Histochem 107(2): 105-12. 

Abstract
The current study identified for the first time calretinin expression in abnormal Sertoli cells of azoospermic men who underwent testicular biopsy for sperm recovery and application of the retrieved sperm by in vitro fertilization techniques. Testicular biopsies with various spermatogenic impairments were evaluated immunohistochemically for the expression of the calretinin calcium-binding protein and the marker for immaturity of Sertoli cells, cytokeratin-18 (CK-18). Distribution of the markers was assessed in testes demonstrating a histological phenotype of mixed atrophy, Sertoli cell-only, or normal spermatogenesis (obstructive-azoospermia) and in men carrying a deletion in the azoospermia factor region located on the Y chromosome. Calretinin-immunopositive immature Sertoli cells revealed by co-localization of both markers, calretinin and CK-18, were identified in the mixed atrophy group in seminiferous tubules demonstrating spermatogenic failure. Sertoli cells expressing both markers were rarely detected in all other groups. Leydig cells in all the assessed biopsies expressed calretinin and served as a built-in control for immunoreactivity. This pattern of calretinin-selective expression in immature Sertoli cells suggests a functional relationship between calretinin expression and the degree of Sertoli cell differentiation. Disorders of Sertoli cell differentiation as indicated by calretinin and/or CK-18 expression contribute to the multifactorial mechanisms underlying spermatogenic failure. PMID: [15950053] 

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Figures for illustrating the function of this protein/gene
Function
Calretinin is a calcium-binding protein which isabundant in auditory neurons. 
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Subcellular Location
 
Tissue Specificity
Brain. 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IDA:HPA.
GO:0005509 F:calcium ion binding IEA:InterPro.
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Interpro
IPR011992;    EF-hand-like_dom.
IPR018247;    EF_Hand_1_Ca_BS.
IPR018249;    EF_HAND_2.
IPR002048;    EF_hand_Ca-bd.
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Pfam
SMART
SM00054;    EFh;    5.
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PROSITE
PS00018;    EF_HAND_1;    5.
PS50222;    EF_HAND_2;    5.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    271       Calretinin.
                             /FTId=PRO_0000073479.
DOMAIN       16     51       EF-hand 1.
DOMAIN       63     98       EF-hand 2.
DOMAIN      107    142       EF-hand 3.
DOMAIN      151    186       EF-hand 4.
DOMAIN      195    230       EF-hand 5.
DOMAIN      235    270       EF-hand 6.
CA_BIND      29     40       1 (Probable).
CA_BIND      76     87       2; possibly ancestral (Probable).
CA_BIND     120    131       3 (Probable).
CA_BIND     164    175       4 (Probable).
CA_BIND     208    219       5 (Probable).
CA_BIND     251    262       6; possibly ancestral (Probable).
CONFLICT    110    110       A -> T (in Ref. 5; AAH15484).
CONFLICT    235    235       M -> I (in Ref. 2; CAA39991).
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Nucleotide Sequence
Length: 1426 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 271 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
KRT1HPRD 
TUBA4AHPRD 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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