SG00000616

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000616

UniProt Accession

BASI_HUMAN;P35613;A6NJW1;D3YLG5;Q7Z796;Q8IZL7;

Theoretical PI

5.56

Molecular Weight

42200 Da

Genbank Nucleotide ID

X64364; D45131; L10240; M87879; L20471; AF042854; AF042848; AF042849; AF042850; AF042851; AF042852; AF042853; AB072923; AB085790; AF548371; GU557064; GU557065; AY358113; AY942196; AC005559; CH471242; CH471242; BC009040;

Genbank Protein ID

CAA45716.1; BAA08109.1; AAA68936.1; AAA91084.1; AAB41120.1; AAD10704.1; AAD10704.1; AAD10704.1; AAD10704.1; AAD10704.1; AAD10704.1; AAD10704.1; BAB88938.1; BAC76828.1; AAN40694.1; ADD31881.1; ADD31882.1; AAQ88480.1; AAX20110.1; AAC33279.1; EAW61181.1; EAW61185.1; AAH09040.1;

Gene Name

BSG

Gene Synonyms/Alias

ORFNames=UNQ6505/PRO21383

Protein Name

Basigin

Protein Synonyms/Alias

5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer;EMMPRIN Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor;TCSF CD_antigen=CD147;Flags: Precursor

Organism

Homo sapiens (Human)

NCBI Taxonomy ID

9606

Chromosome Location

chr:19;571310-583492;1

View in Ensembl genome browser

Function in Stage

Function in Cell Type

Description

These data suggest that CD147 regulates migration of spermatogonia and spermatocytes via induction of MMP-2 production during spermatogenesis.

The information of related literatures

1. H. Chen, K. L. Fok, S. Yu, J. Jiang, Z. Chen, Y. Gui, Z. Cai and H. C. Chan (2011) CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis. Mol Hum Reprod 17(7): 405-14.

Abstract
Spermatogenesis is a highly programmed process that requires the degradation of the extracellular matrix and the remodeling of tight junctions (TJ) to facilitate differentiating germ cell migration. Matrix metalloproteinases (MMPs) are essential in regulating Sertoli cell TJ in the testis. CD147 is known to stimulate the production of MMPs in tumor metastasis and its knockout mice are infertile. However, the functional relationship between CD147 and MMPs in spermatogenesis has not been investigated. In the present study, we examined the expression profile of CD147 and MMPs during mouse testicular development by RT-PCR, western blot and immunofluorescence staining. We also examined CD147 involvement in the production of MMP-2 and the migration of germ cells (GC-1 and GC-2 cells) using CD147 antibody or synthetic microRNA mimics-mediated knockdown. The results showed that CD147 was present at all stages of testicular development from 7 to 56 days post-partum (dpp). CD147 expression was found to increase after 21 days from moderate levels in 7 and 14 days. Of the eight MMPs studied, MMP-2, MMP-7, MMP-9 and MMP-23 were detected to have changes in expression during testicular development, with MMP-2 showing the largest change. CD147 and MMP-2 were co-localized in spermatogonia, spermatocytes and round spermatids in mouse testis, while in human testis, they were co-localized in spermatocytes and round spermatids. MMP-2 expression and migration of GC-1 and GC-2 cells were reduced by interfering with CD147 expression and function in vitro. These data suggest that CD147 regulates migration of spermatogonia and spermatocytes via induction of MMP-2 production during spermatogenesis. PMID: [21343160]

Figures for illustrating the function of this protein/gene

	Ref: H. Chen, K. L. Fok, S. Yu, J. Jiang, Z. Chen, Y. Gui, Z. Cai and H. C. Chan (2011) CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis. Mol Hum Reprod 17(7): 405-14. PMID: [21343160]
	Ref: H. Chen, K. L. Fok, S. Yu, J. Jiang, Z. Chen, Y. Gui, Z. Cai and H. C. Chan (2011) CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis. Mol Hum Reprod 17(7): 405-14. PMID: [21343160]
	Ref: H. Chen, K. L. Fok, S. Yu, J. Jiang, Z. Chen, Y. Gui, Z. Cai and H. C. Chan (2011) CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis. Mol Hum Reprod 17(7): 405-14. PMID: [21343160]

Function

Plays pivotal roles in spermatogenesis, embryoimplantation, neural network formation and tumor progression.Stimulates adjacent fibroblasts to produce matrixmetalloproteinases (MMPS). May target monocarboxylate transportersSLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinalpigment epithelium and neural retina. Seems to be a receptor foroligomannosidic glycans. In vitro, promotes outgrowth ofastrocytic processes (By similarity).

Subcellular Location

Cell membrane; Single-pass type I membraneprotein. Melanosome. Note=Colocalizes with SLC16A1 and SLC16A8 (Bysimilarity). Identified by mass spectrometry in melanosomefractions from stage I to stage IV.

Tissue Specificity

Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumorcells but not in proliferating blood vessels in malignant gliomas.

Gene Ontology

GO ID	GO term	Evidence
GO:0002080	C:acrosomal membrane	IEA:Compara.
GO:0000139	C:Golgi membrane	TAS:Reactome.
GO:0016021	C:integral to membrane	IEA:UniProtKB-KW.
GO:0042470	C:melanosome	IEA:UniProtKB-SubCell.
GO:0045121	C:membrane raft	IEA:Compara.
GO:0005739	C:mitochondrion	IEA:Compara.
GO:0005886	C:plasma membrane	TAS:Reactome.
GO:0042383	C:sarcolemma	IEA:Compara.
GO:0005537	F:mannose binding	IEA:UniProtKB-KW.
GO:0007596	P:blood coagulation	TAS:Reactome.
GO:0007166	P:cell surface receptor signaling pathway	TAS:ProtInc.
GO:0046697	P:decidualization	IEA:Compara.
GO:0007566	P:embryo implantation	IEA:Compara.
GO:0050900	P:leukocyte migration	TAS:Reactome.
GO:0042475	P:odontogenesis of dentin-containing tooth	IEA:Compara.
GO:0006090	P:pyruvate metabolic process	TAS:Reactome.
GO:0051591	P:response to cAMP	IEA:Compara.
GO:0046689	P:response to mercury ion	IEA:Compara.
GO:0043434	P:response to peptide hormone stimulus	IEA:Compara.

Interpro

IPR007110;    Ig-like.
IPR013783;    Ig-like_fold.
IPR013098;    Ig_I-set.
IPR003599;    Ig_sub.
IPR003598;    Ig_sub2.
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Pfam

PF07679; I-set; 2.
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SMART

SM00409; IG; 1.
SM00408; IGc2; 1.
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PROSITE

PS50835; IG_LIKE; 3.
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PRINTS

Created Date

18-Oct-2012

Record Type

Experiment identified

Protein sequence Annotation

SIGNAL        1     21
CHAIN        22    385       Basigin.
                             /FTId=PRO_0000014518.
TOPO_DOM    138    323       Extracellular (Potential).
TRANSMEM    324    344       Helical; (Potential).
TOPO_DOM    345    385       Cytoplasmic (Potential).
DOMAIN      138    219       Ig-like C2-type.
DOMAIN      221    315       Ig-like V-type.
COMPBIAS    356    359       Poly-Asp.
MOD_RES     362    362       Phosphoserine.
MOD_RES     368    368       Phosphoserine.
CARBOHYD    160    160       N-linked (GlcNAc...).
CARBOHYD    268    268       N-linked (GlcNAc...).
CARBOHYD    302    302       N-linked (GlcNAc...) (Potential).
DISULFID    157    203
DISULFID    242    301
VAR_SEQ       1    209       Missing (in isoform 3).
                             /FTId=VSP_043225.
VAR_SEQ       1     11       MAAALFVLLGF -> MKQSDASPQER (in isoform
                             4).
                             /FTId=VSP_043226.
VAR_SEQ      12    191       Missing (in isoform 4).
                             /FTId=VSP_043227.
VAR_SEQ      24    139       Missing (in isoform 2).
                             /FTId=VSP_011501.
VARIANT     208    208       E -> K (in Ok(A-)).
                             /FTId=VAR_013574.
VARIANT     269    269       G -> V (in dbSNP:rs1803203).
                             /FTId=VAR_011720.
CONFLICT    328    328       F -> L (in Ref. 2; BAC76828).
STRAND       24     27
STRAND       32     35
STRAND       40     47
STRAND       53     62
HELIX        74     77
STRAND       79     95
HELIX       100    102
STRAND      104    111
STRAND      116    118
STRAND      128    137
STRAND      142    149
STRAND      152    159
STRAND      166    173
STRAND      176    181
STRAND      186    192
HELIX       194    196
STRAND      199    207
STRAND      210    217
STRAND      224    226
STRAND      228    233
STRAND      238    243
STRAND      252    257
STRAND      260    262
HELIX       270    272
STRAND      274    279
STRAND      282    287
TURN        292    294
STRAND      295    305
STRAND      308    319
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Nucleotide Sequence

Length: 1638 bp Go to nucleotide: FASTA

Protein Sequence

Length: 385 bp Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
2AAB_HUMAN	PPP2R1B	HPRD
A8K220_HUMAN	PPIA	HPRD
ADR1_HUMAN	ADIPOR1	String
A8K3V5_HUMAN	_	BioGRID
ADR1_HUMAN	ADIPOR1	String
ATX10_HUMAN	ATXN10	BioGRID
B4DG05	_	BioGRID
B4DJ67_HUMAN	_	BioGRID
B4DKS0_HUMAN	_	BioGRID
B4DN15_HUMAN	_	BioGRID
B4DPX7_HUMAN	_	BioGRID
BASI_HUMAN	BSG	HPRD
BASI_HUMAN	BSG	HPRD
CAV1_HUMAN	CAV1	HPRD
CDIPT_HUMAN	CDIPT	String
DAPF_BACAN	dapF	IntAct
E7EPY8_HUMAN	SLC16A4	BioGRID
MMP1_HUMAN	MMP1	BioGRID
MOT1_HUMAN	SLC16A1	BioGRID
MOT5_HUMAN	SLC16A4	BioGRID
PDLI7_HUMAN	PDLIM7	HPRD
Q2TNI1_HUMAN	CAV1	HPRD
Q53G95_HUMAN	_	BioGRID
Q53GE1_HUMAN	RANBP3	BioGRID
Q59E85_HUMAN	_	HPRD
Q8WU09_HUMAN	SLC16A4	BioGRID
RANB3_HUMAN	RANBP3	BioGRID
RFXK_HUMAN	RFXANK	BioGRID
UBC_HUMAN	UBC	BioGRID
CDIPT_HUMAN	CDIPT	String
CONA_CANEN	_	IntAct
E9PP86_HUMAN	USP50	BioGRID
GTR4_HUMAN	SLC2A4	HPRD
HAUS4_MOUSE	Haus4	IntAct
HGS_HUMAN	HGS	BioGRID
MMP1_HUMAN	MMP1	HPRD
MOT1_HUMAN	SLC16A1	HPRD
MOT4_HUMAN	SLC16A3	HPRD
PDLI7_HUMAN	PDLIM7	IntAct
PPIA_HUMAN	PPIA	HPRD
PPIL2_HUMAN	PPIL2	HPRD
Q05BQ3_HUMAN	SLC2A4	HPRD
Q53G95_HUMAN	_	HPRD
Q7CL39_YERPE	mviM6	IntAct
RANB3_HUMAN	RANBP3	IntAct
RFXK_HUMAN	RFXANK	IntAct
UBC_HUMAN	UBC	BioGRID
UBE2A_HUMAN	UBE2A	IntAct
UBP50_HUMAN	USP50	IntAct

Other Protein-Protein interaction resources

String database

View Microarray data

Temporarily unavailable

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