SG ID

SG00000623 

UniProt Accession

ZN645_HUMAN;Q8N7E2;A0AV29;A0AV31;Q6DJY9;  

Theoretical PI

9.57  

Molecular Weight

48785 Da  

Genbank Nucleotide ID

AK098601; BX293560; BC074910; BC126190; BC126192;  

Genbank Protein ID

BAC05348.1; CAI40652.1; AAH74910.1; AAI26191.1; AAI26193.1; 

Gene Name

ZNF645 

Gene Synonyms/Alias

 

Protein Name

E3 ubiquitin-protein ligase ZNF645 

Protein Synonyms/Alias

EC=6.3.2.- Zinc finger protein 645; 

Homo sapiens (Human) 

9606 

we suggest that ZNF645 might act as an E3 ubiquitin-protein ligase and play a role in human sperm production and quality control. 

The information of related literatures

1. Y. Q. Liu, G. Bai, H. Zhang, D. Su, D. C. Tao, Y. Yang, Y. X. Ma and S. Z. Zhang (2010) Human RING finger protein ZNF645 is a novel testis-specific E3 ubiquitin ligase. Asian J Androl 12(5): 658-66. 

Abstract
A large number of testis-specific genes are involved in the complex process of mammalian spermatogenesis. Identification of these genes and their roles is important for understanding the mechanisms underlying spermatogenesis. Here we report on a novel human RING finger protein, ZNF645, which contains a C3HC4 RING finger domain, a C2H2 zinc-finger domain, and a proline-rich region, indicating that it has a structure similar to that of the c-Cbl-like protein Hakai. ZNF645 was exclusively expressed in normal human testicular tissue. Immunohistochemical analysis confirmed that ZNF645 protein was present in spermatocytes, round and elongated spermatids, and Leydig cells. Immunofluorescence staining of mature sperms further showed that the ZNF645 protein was localized over the postacrosomal perinuclear theca region and the entire length of sperm tail. An in vitro ubiquitination assay indicated that the RING finger domain of the ZNF645 protein had E3 ubiquitin ligase activity. Therefore, we suggest that ZNF645 might act as an E3 ubiquitin-protein ligase and play a role in human sperm production and quality control. PMID: [20657603] 

Figures for illustrating the function of this protein/gene

Function

E3 ubiquitin ligase catalyzing the covalent attachmentof ubiquitin moieties onto substrate proteins. 

Subcellular Location

Cytoplasm. Note=Localized over thepostacrosomal perinuclear theca region and the entire length ofsperm tail. 

Tissue Specificity

Exclusively expressed in testis and sperm,including spermatocytes, round and elongated spermatids, andLeydig cells. 

Gene Ontology

GO ID	GO term	Evidence
GO:0005737	C:cytoplasm	IEA:UniProtKB-SubCell.
GO:0016874	F:ligase activity	IEA:UniProtKB-KW.
GO:0008270	F:zinc ion binding	IEA:InterPro.

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Interpro

IPR007087;    Znf_C2H2.
IPR015880;    Znf_C2H2-like.
IPR001841;    Znf_RING.
IPR013083;    Znf_RING/FYVE/PHD.
IPR017907;    Znf_RING_CS.
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Pfam

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SMART

SM00184;    RING;    1.
SM00355;    ZnF_C2H2;    1.
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PROSITE

PS00518;    ZF_RING_1;    1.
PS50089;    ZF_RING_2;    1.
PS00028;    ZINC_FINGER_C2H2_1;    FALSE_NEG.
PS50157;    ZINC_FINGER_C2H2_2;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 1517 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 425 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt	Gene Symbol	Ref Databases
PRKN2_HUMAN	PARK2	String
PRKN2_HUMAN	PARK2	String

View Microarray data

Temporarily unavailable 

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