SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000632 
UniProt Accession
Theoretical PI
10.19  
Molecular Weight
12063 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
BPY2C 
Gene Synonyms/Alias
VCY2C 
Protein Name
Testis-specific basic protein Y 2 
Protein Synonyms/Alias
Basic charge, Y-linked 2; Variably charged protein Y 2; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:Y;25130410-25151612;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
The impaired expression of VCY2 in infertile men suggests its involvement in the pathogenesis of male infertility.  
The information of related literatures
1. R. Behulova, L. Strhakova, I. Boronova, A. Cibulkova, M. Konecny, L. Danisovic and V. Repiska (2011) DNA analysis of Y chromosomal AZF region in Slovak population with fertility disorders. Bratisl Lek Listy 112(4): 183-7. 

Abstract
BACKGROUND PMID: [21585124] 

2. J. Y. Tse, E. Y. Wong, A. N. Cheung, W. S. O, P. C. Tam and W. S. Yeung (2003) Specific expression of VCY2 in human male germ cells and its involvement in the pathogenesis of male infertility. Biol Reprod 69(3): 746-51. 

Abstract
Abnormal spermatogenesis in men with Y-chromosome microdeletions suggests that genes important for spermatogenesis have been removed from these individuals. VCY2 is a testis-specific gene that locates in the most frequently deleted azoospermia factor c region in the Y chromosome. We have raised an antiserum to VCY2 and used it to characterize the localization of VCY2 in human testis. Using Western blot analysis, the affinity-purified polyclonal VCY2 antibody gave a single specific band of approximately 14 kDa in size, corresponding to the expected size of VCY2 in all the collected human testicular biopsy specimens with normal spermatogenesis. Immunohistochemical analyses showed that VCY2 localized to the nuclei of spermatogonia, spermatocytes, and round spermatids, except elongated spermatids. At the ultrastructural level, VCY2 expression was found in the nucleus of human ejaculated spermatozoa. To determine the possible relationship of VCY2 with the pathogenesis of male infertility, we examined a group of infertile men with and without Y-chromosome microdeletions and with known testicular pathology using VCY2 antibody. VCY2 was weakly expressed at the spermatogonia and immunonegative in spermatocytes and round spermatids in testicular biopsy specimens with maturation arrest or hypospermatogenesis. The specific localization of the protein in germ cell nuclei indicates that VCY2 is likely to function in male germ cell development. The impaired expression of VCY2 in infertile men suggests its involvement in the pathogenesis of male infertility. PMID: [12724276] 

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Figures for illustrating the function of this protein/gene
Ref: J. Y. Tse, E. Y. Wong, A. N. Cheung, W. S. O, P. C. Tam and W. S. Yeung (2003) Specific expression of VCY2 in human male germ cells and its involvement in the pathogenesis of male infertility. Biol Reprod 69(3): 746-51. PMID: [12724276]
Ref: J. Y. Tse, E. Y. Wong, A. N. Cheung, W. S. O, P. C. Tam and W. S. Yeung (2003) Specific expression of VCY2 in human male germ cells and its involvement in the pathogenesis of male infertility. Biol Reprod 69(3): 746-51. PMID: [12724276]
Function
 
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Subcellular Location
 
Tissue Specificity
Expressed exclusively in testis. Expressed inejaculated spermatozoa of germ cell. Expressed in the nuclei ofspermatogonia, spermatocytes, and round spermatids, exceptelongated spermatids (at protein level). 
Gene Ontology
GO IDGO termEvidence
GO:0005634 C:nucleus IDA:HGNC.
GO:0007338 P:single fertilization TAS:ProtInc.
GO:0007283 P:spermatogenesis IMP:HGNC.
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Interpro
Pfam
SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    106       Testis-specific basic protein Y 2.
                             /FTId=PRO_0000184664.
CONFLICT    101    101       R -> K (in Ref. 1; AAC51828).
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Nucleotide Sequence
Length: 1212 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 106 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
MAP1SHPRD 
UBE3AHPRD 
MAP1SHPRD 
UBE3ABioGRID 
UBE3ABioGRID 
UBE3AHPRD 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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