SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000639 
UniProt Accession
Theoretical PI
5.38  
Molecular Weight
133367 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
JAG2 
Gene Synonyms/Alias
 
Protein Name
Protein jagged-2 
Protein Synonyms/Alias
Jagged2hJ2Flags: Precursor 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:14;105607318-105635161;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
In conclusion, the results presented in this study offer the possibility that Notch 1/Jagged 2 signaling system plays an important role for male germ cells to differentiate or at least to survive in the rat testis and fails to express in the testis of spermatogenic maturation arrest patients. 
The information of related literatures
1. T. Hayashi, Y. Kageyama, K. Ishizaka, G. Xia, K. Kihara and H. Oshima (2001) Requirement of Notch 1 and its ligand jagged 2 expressions for spermatogenesis in rat and human testes. J Androl 22(6): 999-1011. 

Abstract
It has already been demonstrated that the Notch signaling system is essential for gametogenesis in the adult germ line of Caenorhabditis elegans. However, the role of the Notch signaling system in mammalian spermatogenesis has not been well investigated. Recently, it has been revealed that this signaling system is expressed in the mammalian testis by showing coexpression of Jagged 2 and its receptor, Notch 1, is consistent with Notch 1 being a cognate receptor for Jagged 2 in the mammalian testis. Therefore, we investigated expressions of messenger RNAs of Notch 1 and Jagged 2 in the testicular tissues of developing Sprague-Dawley rats by reverse transcription-polymerase chain reaction and Northern blot analysis, expressions of their proteins in the testicular tissues of developing rats, fertile human controls and infertile human patients with maturation arrest by immunohistochemistry, and effects of antibodies to this system by culturing rat testicular tissues with these antibodies. Transcripts of Notch 1 and Jagged 2 in the rat testis were positive throughout the examined period; these intensities became higher at day 13 after birth, coincidence with the formation of spermatocytes, and peaked at day 19 after birth. Expressions of Notch 1 and Jagged 2 were recognized at first in the perinuclear regions of spermatocytes in the rat testis as a round structure at day 19 after birth and thereafter in further differentiated germ cells as meiosis proceeded. In the adult rat testis, positive staining was present as a round structure in spermatocytes, as a typical horseshoe-shaped structure in round spermatids, and as a covering structure spreading around the nucleus of elongated spermatids, but not in spermatozoa. Notch 1 was recognized in the vacuole of the Golgi complex of primary spermatocytes and the acrosome of elongated spermatids with electron microscopy. When rat testicular tissues were cultured with anti-Notch 1 or anti-Jagged 2 antibody, round and elongated spermatids decreased after 5 and 7 days of culture, respectively, and disappeared at around 9 and 12 days of culture, respectively, with shrinkage of the diameter of seminiferous tubules. Spermatocytes, however, increased after 11 days of culture. Expressions of both proteins have been detected in the testicular tissues of human fertile controls as in the rat testicular tissues. However, Notch 1 expression has not been detected in testicular tissues of 11 patients with maturation arrest, whereas Jagged 2 expression has been recognized in all of them. In conclusion, the results presented in this study offer the possibility that Notch 1/Jagged 2 signaling system plays an important role for male germ cells to differentiate or at least to survive in the rat testis and fails to express in the testis of spermatogenic maturation arrest patients. PMID: [11700865] 

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Figures for illustrating the function of this protein/gene
Function
Putative Notch ligand involved in the mediation of Notchsignaling. Involved in limb development (By similarity). 
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Subcellular Location
Membrane; Single-pass type I membraneprotein. 
Tissue Specificity
Expressed in heart, placenta and skeletalmuscle and to a lesser extend in pancreas. Very low expression inbrain, lung, liver and kidney. 
Gene Ontology
GO IDGO termEvidence
GO:0005887 C:integral to plasma membrane ISS:UniProtKB.
GO:0005624 C:membrane fraction IEA:Compara.
GO:0005509 F:calcium ion binding IEA:InterPro.
GO:0009912 P:auditory receptor cell fate commitment ISS:UniProtKB.
GO:0007049 P:cell cycle NAS:UniProtKB.
GO:0001701 P:in utero embryonic development IEA:Compara.
GO:0016331 P:morphogenesis of embryonic epithelium IEA:Compara.
GO:0007220 P:Notch receptor processing TAS:Reactome.
GO:0007219 P:Notch signaling pathway NAS:UniProtKB.
GO:0042475 P:odontogenesis of dentin-containing tooth IEA:Compara.
GO:0042981 P:regulation of apoptotic process IEA:Compara.
GO:0030155 P:regulation of cell adhesion IEA:Compara.
GO:0030334 P:regulation of cell migration NAS:UniProtKB.
GO:0042127 P:regulation of cell proliferation IDA:UniProtKB.
GO:0003016 P:respiratory system process IEA:Compara.
GO:0001501 P:skeletal system development IEA:Compara.
GO:0007283 P:spermatogenesis IEP:UniProtKB.
GO:0045061 P:thymic T cell selection IDA:UniProtKB.
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Interpro
IPR001774;    DSL.
IPR000742;    EG-like_dom.
IPR006210;    EGF-like.
IPR001881;    EGF-like_Ca-bd.
IPR013032;    EGF-like_CS.
IPR006209;    EGF-like_dom.
IPR000152;    EGF-type_Asp/Asn_hydroxyl_site.
IPR018097;    EGF_Ca-bd_CS.
IPR026219;    Jagged/Serrate.
IPR011651;    Notch_ligand_N.
IPR001007;    VWF_C.
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Pfam
PF01414;    DSL;    1.
PF00008;    EGF;    9.
PF07645;    EGF_CA;    1.
PF12661;    hEGF;    2.
PF07657;    MNNL;    1.
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SMART
SM00051;    DSL;    1.
SM00181;    EGF;    6.
SM00179;    EGF_CA;    9.
SM00214;    VWC;    1.
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PROSITE
PS00010;    ASX_HYDROXYL;    10.
PS51051;    DSL;    1.
PS00022;    EGF_1;    16.
PS01186;    EGF_2;    12.
PS50026;    EGF_3;    15.
PS01187;    EGF_CA;    8.
PS01208;    VWFC_1;    FALSE_NEG.
PS50184;    VWFC_2;    1.
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PRINTS
PR02059;    JAGGEDFAMILY.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
SIGNAL        1     23       Potential.
CHAIN        24   1238       Protein jagged-2.
                             /FTId=PRO_0000007629.
TOPO_DOM     27   1080       Extracellular (Potential).
TRANSMEM   1081   1101       Helical; (Potential).
TOPO_DOM   1102   1238       Cytoplasmic (Potential).
DOMAIN      196    240       DSL.
DOMAIN      241    274       EGF-like 1.
DOMAIN      275    305       EGF-like 2; atypical.
DOMAIN      307    345       EGF-like 3.
DOMAIN      347    383       EGF-like 4.
DOMAIN      385    421       EGF-like 5; calcium-binding (Potential).
DOMAIN      423    459       EGF-like 6; calcium-binding (Potential).
DOMAIN      461    496       EGF-like 7; calcium-binding (Potential).
DOMAIN      498    534       EGF-like 8.
DOMAIN      536    572       EGF-like 9.
DOMAIN      574    634       EGF-like 10; atypical.
DOMAIN      636    672       EGF-like 11; calcium-binding (Potential).
DOMAIN      674    710       EGF-like 12; calcium-binding (Potential).
DOMAIN      712    748       EGF-like 13.
DOMAIN      751    787       EGF-like 14.
DOMAIN      789    825       EGF-like 15; calcium-binding (Potential).
DOMAIN      827    863       EGF-like 16; calcium-binding (Potential).
DOMAIN      870    944       VWFC.
CARBOHYD    153    153       N-linked (GlcNAc...) (Potential).
CARBOHYD    570    570       N-linked (GlcNAc...) (Potential).
CARBOHYD    619    619       N-linked (GlcNAc...) (Potential).
CARBOHYD    752    752       N-linked (GlcNAc...) (Potential).
CARBOHYD   1058   1058       N-linked (GlcNAc...) (Potential).
DISULFID    198    207       By similarity.
DISULFID    211    223       By similarity.
DISULFID    231    240       By similarity.
DISULFID    245    256       By similarity.
DISULFID    249    262       By similarity.
DISULFID    264    273       By similarity.
DISULFID    276    287       By similarity.
DISULFID    282    293       By similarity.
DISULFID    295    304       By similarity.
DISULFID    311    323       By similarity.
DISULFID    317    333       By similarity.
DISULFID    335    344       By similarity.
DISULFID    351    362       By similarity.
DISULFID    356    371       By similarity.
DISULFID    373    382       By similarity.
DISULFID    389    400       By similarity.
DISULFID    394    409       By similarity.
DISULFID    411    420       By similarity.
DISULFID    427    438       By similarity.
DISULFID    432    447       By similarity.
DISULFID    449    458       By similarity.
DISULFID    465    475       By similarity.
DISULFID    469    484       By similarity.
DISULFID    486    495       By similarity.
DISULFID    502    513       By similarity.
DISULFID    507    522       By similarity.
DISULFID    524    533       By similarity.
DISULFID    540    551       By similarity.
DISULFID    545    560       By similarity.
DISULFID    562    571       By similarity.
DISULFID    589    612       Potential.
DISULFID    606    622       Potential.
DISULFID    624    633       By similarity.
DISULFID    640    651       By similarity.
DISULFID    645    660       By similarity.
DISULFID    662    671       By similarity.
DISULFID    678    689       By similarity.
DISULFID    683    698       By similarity.
DISULFID    700    709       By similarity.
DISULFID    716    727       By similarity.
DISULFID    721    736       By similarity.
DISULFID    738    747       By similarity.
DISULFID    755    766       By similarity.
DISULFID    760    775       By similarity.
DISULFID    777    786       By similarity.
DISULFID    793    804       By similarity.
DISULFID    798    813       By similarity.
DISULFID    815    824       By similarity.
DISULFID    831    842       By similarity.
DISULFID    836    851       By similarity.
DISULFID    853    862       By similarity.
VAR_SEQ     424    461       Missing (in isoform Short).
                             /FTId=VSP_001395.
VARIANT     501    501       E -> K (in dbSNP:rs1057744).
                             /FTId=VAR_028113.
VARIANT     538    538       D -> N (in dbSNP:rs9972231).
                             /FTId=VAR_048986.
CONFLICT      8     12       RLPRR -> AFPPA (in Ref. 1; AAB71189).
CONFLICT    119    119       A -> P (in Ref. 1; AAB71189).
CONFLICT    129    129       L -> F (in Ref. 1; AAB71189).
CONFLICT    384    384       L -> SA (in Ref. 4; CAA74706).
CONFLICT    424    426       ANE -> VND (in Ref. 1; AAB71189).
CONFLICT   1235   1235       A -> V (in Ref. 2; AAB61285).
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Nucleotide Sequence
Length: 4749 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1238 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Other Protein-Protein interaction resources
String database  
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