SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000735 
UniProt Accession
Theoretical PI
5.56  
Molecular Weight
42445 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Bsg 
Gene Synonyms/Alias
 
Protein Name
Basigin 
Protein Synonyms/Alias
Basic immunoglobulin superfamily; HT7 antigen; Membrane glycoprotein gp42; CD_antigen=CD147;Flags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:-;---;-
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
CD147 regulates migration of spermatogonia and spermatocytes via induction of MMP-2 production during spermatogenesis. 
The information of related literatures
1. H. Chen, K. L. Fok, S. Yu, J. Jiang, Z. Chen, Y. Gui, Z. Cai and H. C. Chan (2011) CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis. Mol Hum Reprod 17(7): 405-14. 

Abstract
Spermatogenesis is a highly programmed process that requires the degradation of the extracellular matrix and the remodeling of tight junctions (TJ) to facilitate differentiating germ cell migration. Matrix metalloproteinases (MMPs) are essential in regulating Sertoli cell TJ in the testis. CD147 is known to stimulate the production of MMPs in tumor metastasis and its knockout mice are infertile. However, the functional relationship between CD147 and MMPs in spermatogenesis has not been investigated. In the present study, we examined the expression profile of CD147 and MMPs during mouse testicular development by RT-PCR, western blot and immunofluorescence staining. We also examined CD147 involvement in the production of MMP-2 and the migration of germ cells (GC-1 and GC-2 cells) using CD147 antibody or synthetic microRNA mimics-mediated knockdown. The results showed that CD147 was present at all stages of testicular development from 7 to 56 days post-partum (dpp). CD147 expression was found to increase after 21 days from moderate levels in 7 and 14 days. Of the eight MMPs studied, MMP-2, MMP-7, MMP-9 and MMP-23 were detected to have changes in expression during testicular development, with MMP-2 showing the largest change. CD147 and MMP-2 were co-localized in spermatogonia, spermatocytes and round spermatids in mouse testis, while in human testis, they were co-localized in spermatocytes and round spermatids. MMP-2 expression and migration of GC-1 and GC-2 cells were reduced by interfering with CD147 expression and function in vitro. These data suggest that CD147 regulates migration of spermatogonia and spermatocytes via induction of MMP-2 production during spermatogenesis. PMID: [21343160] 

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Figures for illustrating the function of this protein/gene
Ref: H. Chen, K. L. Fok, S. Yu, J. Jiang, Z. Chen, Y. Gui, Z. Cai and H. C. Chan (2011) CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis. Mol Hum Reprod 17(7): 405-14. PMID: [21343160]
Ref: H. Chen, K. L. Fok, S. Yu, J. Jiang, Z. Chen, Y. Gui, Z. Cai and H. C. Chan (2011) CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis. Mol Hum Reprod 17(7): 405-14. PMID: [21343160]
Ref: H. Chen, K. L. Fok, S. Yu, J. Jiang, Z. Chen, Y. Gui, Z. Cai and H. C. Chan (2011) CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis. Mol Hum Reprod 17(7): 405-14. PMID: [21343160]
Function
Plays pivotal roles in spermatogenesis, embryoimplantation, neural network formation and tumor progression.Stimulates adjacent fibroblasts to produce matrixmetalloproteinases (MMPS). May target monocarboxylate transportersSLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinalpigment epithelium and neural retina. Seems to be a receptor foroligomannosidic glycans. In vitro, promotes outgrowth ofastrocytic processes. 
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Subcellular Location
Cell membrane; Single-pass type I membraneprotein. Melanosome (By similarity). Note=Colocalizes with SLC16A1and SLC16A8. In spermatozoa, localized on the principal piece ofcaput and in the middle piece during transit in the corpus andcauda epididymides. 
Tissue Specificity
Isoform 1 is specifically expressed in retina.Isoform 2 is widely expressed, including adult organs, embryos andEC cells. Expressed in spermatozoa. 
Gene Ontology
GO IDGO termEvidence
GO:0002080 C:acrosomal membrane IDA:MGI.
GO:0016021 C:integral to membrane IEA:UniProtKB-KW.
GO:0042470 C:melanosome IEA:UniProtKB-SubCell.
GO:0045121 C:membrane raft IEA:Compara.
GO:0005739 C:mitochondrion IEA:Compara.
GO:0042383 C:sarcolemma IEA:Compara.
GO:0005537 F:mannose binding IEA:UniProtKB-KW.
GO:0046697 P:decidualization IEA:Compara.
GO:0007566 P:embryo implantation IEA:Compara.
GO:0042475 P:odontogenesis of dentin-containing tooth IEA:Compara.
GO:0051591 P:response to cAMP IEA:Compara.
GO:0046689 P:response to mercury ion IEA:Compara.
GO:0043434 P:response to peptide hormone stimulus IEA:Compara.
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Interpro
IPR007110;    Ig-like.
IPR013783;    Ig-like_fold.
IPR013098;    Ig_I-set.
IPR003599;    Ig_sub.
IPR003598;    Ig_sub2.
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Pfam
PF07679;    I-set;    1.
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SMART
SM00409;    IG;    1.
SM00408;    IGc2;    1.
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PROSITE
PS50835;    IG_LIKE;    3.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
SIGNAL        1     21
CHAIN        22    389       Basigin.
                             /FTId=PRO_0000014519.
TOPO_DOM     22    325       Extracellular (Potential).
TRANSMEM    326    349       Helical; (Potential).
TOPO_DOM    350    389       Cytoplasmic (Potential).
DOMAIN      138    219       Ig-like C2-type.
DOMAIN      221    319       Ig-like V-type.
MOD_RES     358    358       Phosphothreonine.
MOD_RES     372    372       Phosphoserine (By similarity).
CARBOHYD    160    160       N-linked (GlcNAc...).
CARBOHYD    270    270       N-linked (GlcNAc...).
CARBOHYD    275    275       N-linked (GlcNAc...).
CARBOHYD    306    306       N-linked (GlcNAc...).
CARBOHYD    309    309       N-linked (GlcNAc...).
DISULFID    157    203       By similarity.
DISULFID    242    305       By similarity.
VAR_SEQ      24    139       Missing (in isoform 2).
                             /FTId=VSP_011502.
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Nucleotide Sequence
Length: 1242 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 389 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
YwhabIntAct 
Poldip2String 
Poldip2String 
RYR1MINT 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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