SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000783 
UniProt Accession
Theoretical PI
6.71  
Molecular Weight
57638 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Cyp17a1 
Gene Synonyms/Alias
Cyp17 
Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase 
Protein Synonyms/Alias
EC=1.14.99.9 CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17;Cytochrome P450c17 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:19;46741655-46747464;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Cytochrome P450 17alpha-hydroxylase/17,20-lyase (CYP17) is critical in determining cortisol and sex steroid biosynthesis. CYP17, in addition to its role in steroidogenesis and androgen formation, is present in germ cells where it is essential for sperm function, and deletion of one allele prevents genetic transmission of mutant and wild-type alleles causing infertility followed by change in sexual behavior due to androgen imbalance.  
The information of related literatures
1. Y. Liu, Z. X. Yao, C. Bendavid, C. Borgmeyer, Z. Han, L. R. Cavalli, W. Y. Chan, J. Folmer, B. R. Zirkin, B. R. Haddad, G. I. Gallicano and V. Papadopoulos (2005) Haploinsufficiency of cytochrome P450 17alpha-hydroxylase/17,20 lyase (CYP17) causes infertility in male mice. Mol Endocrinol 19(9): 2380-9. 

Abstract
Cytochrome P450 17alpha-hydroxylase/17,20-lyase (CYP17) is critical in determining cortisol and sex steroid biosynthesis. To investigate how CYP17 functions in vivo, we generated mice with a targeted deletion of CYP17. Although in chimeric mice Leydig cell CYP17 mRNA and intratesticular and circulating testosterone levels were dramatically reduced (80%), the remaining testosterone was sufficient to support spermatogenesis as evidenced by the generation of phenotypical black C57BL/6 mice. However, male chimeras consistently failed to generate heterozygous CYP17 mice and after five matings chimeric mice stopped mating indicating a change in sexual behavior. These results suggested that CYP17 deletion caused a primary phenotype (infertility), probably not due to the anticipated androgen imbalance and a secondary phenotype (change in sexual behavior) due to the androgen imbalance. Surprisingly, CYP17 mRNA was found in mature sperm, and serial analysis of gene expression identified CYP17 mRNA in other testicular germ cells. CYP17 mRNA levels were directly related to percent chimerism. Moreover, more than 50% of the sperm from high-percentage chimeric mice were morphologically abnormal, and half of them failed the swim test. Furthermore, 60% of swimming abnormal sperm was devoid of CYP17. These results suggest that CYP17, in addition to its role in steroidogenesis and androgen formation, is present in germ cells where it is essential for sperm function, and deletion of one allele prevents genetic transmission of mutant and wild-type alleles causing infertility followed by change in sexual behavior due to androgen imbalance. PMID: [15890676] 

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Figures for illustrating the function of this protein/gene
Ref: Y. Liu, Z. X. Yao, C. Bendavid, C. Borgmeyer, Z. Han, L. R. Cavalli, W. Y. Chan, J. Folmer, B. R. Zirkin, B. R. Haddad, G. I. Gallicano and V. Papadopoulos (2005) Haploinsufficiency of cytochrome P450 17alpha-hydroxylase/17,20 lyase (CYP17) causes infertility in male mice. Mol Endocrinol 19(9): 2380-9. PMID: [15890676]
Function
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently todehydroepiandrosterone (DHEA) and androstenedione. Catalyzes boththe 17-alpha-hydroxylation and the 17,20-lyase reaction. Involvedin sexual development during fetal life and at puberty. 
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Subcellular Location
Membrane (Potential). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0030424 C:axon IDA:MGI.
GO:0005783 C:endoplasmic reticulum IEA:Compara.
GO:0016020 C:membrane IEA:UniProtKB-SubCell.
GO:0005792 C:microsome IEA:Compara.
GO:0005739 C:mitochondrion IDA:MGI.
GO:0043025 C:neuronal cell body IDA:MGI.
GO:0009055 F:electron carrier activity IEA:InterPro.
GO:0020037 F:heme binding IEA:InterPro.
GO:0004508 F:steroid 17-alpha-monooxygenase activity IDA:MGI.
GO:0030325 P:adrenal gland development IEA:Compara.
GO:0018879 P:biphenyl metabolic process IEA:Compara.
GO:0071236 P:cellular response to antibiotic IEA:Compara.
GO:0071371 P:cellular response to gonadotropin stimulus IEA:Compara.
GO:0071222 P:cellular response to lipopolysaccharide IEA:Compara.
GO:0018894 P:dibenzo-p-dioxin metabolic process IEA:Compara.
GO:0006704 P:glucocorticoid biosynthetic process IDA:MGI.
GO:0021766 P:hippocampus development IEA:Compara.
GO:0033327 P:Leydig cell differentiation IEA:Compara.
GO:0030728 P:ovulation IEA:Compara.
GO:0018958 P:phenol-containing compound metabolic process IEA:Compara.
GO:0018963 P:phthalate metabolic process IEA:Compara.
GO:0090031 P:positive regulation of steroid hormone biosynthetic process IEA:Compara.
GO:0010034 P:response to acetate IEA:Compara.
GO:0051591 P:response to cAMP IEA:Compara.
GO:0034097 P:response to cytokine stimulus IEA:Compara.
GO:0042493 P:response to drug IEA:Compara.
GO:0060992 P:response to fungicide IEA:Compara.
GO:0009635 P:response to herbicide IEA:Compara.
GO:0017085 P:response to insecticide IEA:Compara.
GO:0010212 P:response to ionizing radiation IEA:Compara.
GO:0051597 P:response to methylmercury IEA:Compara.
GO:0014070 P:response to organic cyclic compound IEA:Compara.
GO:0032526 P:response to retinoic acid IEA:Compara.
GO:0048545 P:response to steroid hormone stimulus IEA:Compara.
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Interpro
IPR001128;    Cyt_P450.
IPR017972;    Cyt_P450_CS.
IPR002401;    Cyt_P450_E_grp-I.
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Pfam
PF00067;    p450;    1.
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SMART
PROSITE
PS00086;    CYTOCHROME_P450;    1.
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PRINTS
PR00463;    EP450I.;   
PR00385;    P450.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    507       Steroid 17-alpha-hydroxylase/17,20 lyase.
                             /FTId=PRO_0000051935.
METAL       441    441       Iron (heme axial ligand) (By similarity).
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Nucleotide Sequence
Length: 1696 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 507 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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