SG ID

SG00000872 

UniProt Accession

BIK_MOUSE;O70337;  

Theoretical PI

7.76  

Molecular Weight

16901 Da  

Genbank Nucleotide ID

AF048838; AL583889; CH467427;  

Genbank Protein ID

AAC40079.1; EDL01409.1; 

Gene Name

Bik 

Gene Synonyms/Alias

Biklk, Blk, Nbk 

Protein Name

Bcl-2-interacting killer 

Protein Synonyms/Alias

Apoptosis inducer NBK; Bik-like killer protein; 

Mus musculus (Mouse) 

10090 

Bik and Bim are related to Bcl-2 (and each other) only by the short BH3 region, which is essential for their proapoptotic function. Bik and Bim share, upstream of Bax, the role of eliminating supernumerary germ cells during the first wave of spermatogenesis, a process vital for normal testicular development.  

The information of related literatures

1. L. Coultas, P. Bouillet, K. L. Loveland, S. Meachem, H. Perlman, J. M. Adams and A. Strasser (2005) Concomitant loss of proapoptotic BH3-only Bcl-2 antagonists Bik and Bim arrests spermatogenesis. EMBO J 24(22): 3963-73. 

Abstract
The BH3-only proteins of the Bcl-2 family initiate apoptosis through the activation of Bax-like relatives. Loss of individual BH3-only proteins can lead either to no phenotype, as in mice lacking Bik, or to marked cell excess, as in the hematopoietic compartment of animals lacking Bim. To investigate whether functional redundancy with Bim might obscure a significant role for Bik, we generated mice lacking both genes. The hematopoietic compartments of bik-/-bim-/- and bim-/- mice were indistinguishable. However, although testes develop normally in mice lacking either Bik or Bim, adult bik-/-bim-/- males were infertile, with reduced testicular cellularity and no spermatozoa. The testis of young bik-/-bim-/- males, like those lacking Bax, exhibited increased numbers of spermatogonia and spermatocytes, although loss of Bik plus Bim blocked spermatogenesis somewhat later than Bax deficiency. The initial excess of early germ cells suggests that spermatogenesis fails because supporting Sertoli cells are overwhelmed. Thus, Bik and Bim share, upstream of Bax, the role of eliminating supernumerary germ cells during the first wave of spermatogenesis, a process vital for normal testicular development. PMID: [16270031] 

Figures for illustrating the function of this protein/gene

Function

Accelerates programmed cell death. Binding to theapoptosis repressors Bcl-X(L), BHRF1 or Bcl-2 suppresses thisdeath-promoting activity. 

Subcellular Location

Endomembrane system; Single-pass membraneprotein (By similarity). Mitochondrion membrane; Single-passmembrane protein. Note=Around the nuclear envelope, and incytoplasmic membranes (By similarity). 

Tissue Specificity

Expressed in testis, kidney, liver, lung andheart. 

Gene Ontology

GO ID	GO term	Evidence
GO:0005740	C:mitochondrial envelope	IDA:MGI.
GO:0006917	P:induction of apoptosis	IDA:MGI.
GO:0008584	P:male gonad development	IGI:MGI.
GO:0032464	P:positive regulation of protein homooligomerization	IEA:Compara.
GO:0090200	P:positive regulation of release of cytochrome c from mitochondria	IEA:Compara.
GO:0007283	P:spermatogenesis	IGI:MGI.

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Interpro

IPR024579;    Bcl2-int_killer.
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Pfam

PF12201;    bcl-2I13;    1.
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SMART

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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 942 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 150 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

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