SG00000980

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000980

UniProt Accession

D3YTQ2_MOUSE;D3YTQ2;

Theoretical PI

6.49

Molecular Weight

1642 Da

Genbank Nucleotide ID

AC090654; AC090962;

Genbank Protein ID

Gene Name

Gata4

Gene Synonyms/Alias

Protein Name

Protein Synonyms/Alias

SubName: Transcription factor GATA-4Flags: Fragment

Organism

Mus musculus (Mouse)

NCBI Taxonomy ID

10090

Chromosome Location

chr:14;63817759-63890529;-1

View in Ensembl genome browser

Function in Stage

premeiotic

Function in Cell Type

leydig_cell

sertoli_cell

Description

Transcription factor GATA4 is expressed in Sertoli and Leydig cells and is required for proper development of the murine fetal testis. GATA4 is a key transcriptional regulator of Sertoli cell function in adult mice. Sertoli cell vacuolation, impaired spermatogenesis, and increased permeability of the blood-testis barrier are demonstrated in sertoli cell knockout GATA4 mice.

The information of related literatures

1. A. Kyronlahti, R. Euler, M. Bielinska, E. L. Schoeller, K. H. Moley, J. Toppari, M. Heikinheimo and D. B. Wilson (2011) GATA4 regulates Sertoli cell function and fertility in adult male mice. Mol Cell Endocrinol 333(1): 85-95.

Abstract
Transcription factor GATA4 is expressed in Sertoli and Leydig cells and is required for proper development of the murine fetal testis. The role of GATA4 in adult testicular function, however, has remained unclear due to prenatal lethality of mice harboring homozygous mutations in Gata4. To characterize the function of GATA4 in the adult testis, we generated mice in which Gata4 was conditionally deleted in Sertoli cells using Cre-LoxP recombination with Amhr2-Cre. Conditional knockout (cKO) mice developed age-dependent testicular atrophy and loss of fertility, which coincided with decreases in the quantity and motility of sperm. Histological analysis demonstrated Sertoli cell vacuolation, impaired spermatogenesis, and increased permeability of the blood-testis barrier. RT-PCR analysis of cKO testes showed decreased expression of germ cell markers and increased expression of testicular injury markers. Our findings support the premise that GATA4 is a key transcriptional regulator of Sertoli cell function in adult mice. PMID: [21172404]

Figures for illustrating the function of this protein/gene

Function

Subcellular Location

Tissue Specificity

Gene Ontology

GO ID	GO term	Evidence
GO:0005634	C:nucleus	IDA:MGI.
GO:0003682	F:chromatin binding	IDA:MGI.
GO:0003705	F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity	IDA:MGI.
GO:0000977	F:RNA polymerase II regulatory region sequence-specific DNA binding	IDA:MGI.
GO:0003181	P:atrioventricular valve morphogenesis	IGI:MGI.
GO:0030509	P:BMP signaling pathway	IDA:MGI.
GO:0060070	P:canonical Wnt receptor signaling pathway	IDA:MGI.
GO:0055007	P:cardiac muscle cell differentiation	IMP:MGI.
GO:0007267	P:cell-cell signaling	IMP:MGI.
GO:0071372	P:cellular response to follicle-stimulating hormone stimulus	IDA:MGI.
GO:0048557	P:embryonic digestive tract morphogenesis	IMP:MGI.
GO:0048617	P:embryonic foregut morphogenesis	IMP:MGI.
GO:0035054	P:embryonic heart tube anterior/posterior pattern specification	IMP:MGI.
GO:0003197	P:endocardial cushion development	IGI:MGI.
GO:0001706	P:endoderm formation	IMP:MGI.
GO:0072148	P:epithelial cell fate commitment	IMP:MGI.
GO:0001702	P:gastrulation with mouth forming second	IMP:MGI.
GO:0001701	P:in utero embryonic development	IMP:MGI.
GO:0060575	P:intestinal epithelial cell differentiation	ISO:MGI.
GO:0060464	P:lung lobe formation	IMP:MGI.
GO:0060045	P:positive regulation of cardiac muscle cell proliferation	IGI:MGI.
GO:0045944	P:positive regulation of transcription from RNA polymerase II promoter	IDA:MGI.
GO:0043627	P:response to estrogen stimulus	IDA:MGI.
GO:0072520	P:seminiferous tubule development	IMP:MGI.
GO:0060008	P:Sertoli cell differentiation	IMP:MGI.
GO:0023019	P:signal transduction involved in regulation of gene expression	IDA:MGI.
GO:0060395	P:SMAD protein signal transduction	IDA:MGI.
GO:0007283	P:spermatogenesis	IMP:MGI.
GO:0035239	P:tube morphogenesis	IGI:MGI.
GO:0003229	P:ventricular cardiac muscle tissue development	IGI:MGI.