SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000995 
UniProt Accession
Theoretical PI
4.56  
Molecular Weight
237323 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Myo10 
Gene Synonyms/Alias
 
Protein Name
Unconventional myosin-X 
Protein Synonyms/Alias
Unconventional myosin-10; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:15;25552280-25743428;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Plays a role in regions of dynamic actin. Myosin X is implicated in the supporting cells during the spermatid morphogenesis. 
The information of related literatures
1. S. Yonezawa, A. Kimura, S. Koshiba, S. Masaki, T. Ono, A. Hanai, S. Sonta, T. Kageyama, T. Takahashi and A. Moriyama (2000) Mouse myosin X. Biochem Biophys Res Commun 271(2): 526-33. 

Abstract
The structure of the coding region of mouse myosin X cDNA was determined. The predicted protein sequence indicated an approximately 240 kDa molecular mass with 2062 amino acids. When aligned with the structure predicted for calf myosin X (GenBank Accession No. U55042), extremely highly conserved pleckstrin homology domains and a myosin tail homology 4 domain were apparent in the tail region, suggesting their importance for myosin X's function. Northern blot analysis revealed the existence of a myosin X mRNA, 8.7 kb in size, in various mouse tissues, while a similar size of human type myosin X mRNA was recognized mainly in the testis. In addition to the adult-type transcripts in mice, a smaller embryo-specific mRNA, 4.8 kb in size, was identified in early to late embryonic stages, suggesting the presence of a shorter myosin X isoform in mouse embryos. In situ hybridization experiments with mouse testis revealed that myosin X mRNA was restricted to Sertoli cells at stages VIII-X of the spermatogenesis cycle, suggesting that myosin X is implicated in the supporting cells during the spermatid morphogenesis. PMID: [10799329] 

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Figures for illustrating the function of this protein/gene
Function
Myosins are actin-based motor molecules with ATPaseactivity. Unconventional myosins serve in intracellular movements.MYO10 binds to actin filaments and actin bundles and functions asplus end-directed motor. The tail domain binds to membranouscompartments containing phosphatidylinositol 3,4,5-trisphosphateor integrins, and mediates cargo transport along actin filaments(By similarity). Regulates cell shape, cell spreading and celladhesion. Stimulates the formation and elongation of filopodia.May play a role in neurite outgrowth and axon guidance. Plays arole in formation of the podosome belt in osteoclasts. 
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Subcellular Location
Cytoplasm, cytosol. Cell projection,lamellipodium (By similarity). Cell projection, ruffle (Bysimilarity). Cytoplasm, cytoskeleton. Cell projection, filopodiumtip (By similarity). Cytoplasm, cell cortex. Cell projection,filopodium membrane; Peripheral membrane protein (By similarity).Note=May be in an inactive, monomeric conformation in the cytosol.Detected in cytoplasmic punctae and in cell projections.Colocalizes with actin fibers. Interacts with microtubules.Undergoes forward and rearward movements within filopodia.Interaction with membranes containing phosphatidylinositol 3,4,5-trisphosphate mediates localization at filopodium membranes (Bysimilarity). 
Tissue Specificity
Detected in heart, kidney, liver, stomach,skeletal muscle, lung, testis and skin. 
Gene Ontology
GO IDGO termEvidence
GO:0005938 C:cell cortex IEA:UniProtKB-SubCell.
GO:0005829 C:cytosol IEA:UniProtKB-SubCell.
GO:0031527 C:filopodium membrane IEA:UniProtKB-SubCell.
GO:0032433 C:filopodium tip ISS:UniProtKB.
GO:0030027 C:lamellipodium IEA:UniProtKB-SubCell.
GO:0016459 C:myosin complex IEA:UniProtKB-KW.
GO:0043005 C:neuron projection IDA:MGI.
GO:0043025 C:neuronal cell body IDA:MGI.
GO:0001726 C:ruffle IEA:UniProtKB-SubCell.
GO:0030898 F:actin-dependent ATPase activity ISS:UniProtKB.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0005547 F:phosphatidylinositol-3,4,5-trisphosphate binding ISS:UniProtKB.
GO:0060002 F:plus-end directed microfilament motor activity ISS:UniProtKB.
GO:0048870 P:cell motility IC:MGI.
GO:0030705 P:cytoskeleton-dependent intracellular transport ISS:UniProtKB.
GO:0022409 P:positive regulation of cell-cell adhesion IGI:MGI.
GO:0008360 P:regulation of cell shape ISS:UniProtKB.
GO:0051489 P:regulation of filopodium assembly ISS:UniProtKB.
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Interpro
IPR019749;    Band_41_domain.
IPR014352;    FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748;    FERM_central.
IPR000299;    FERM_domain.
IPR000048;    IQ_motif_EF-hand-BS.
IPR001609;    Myosin_head_motor_dom.
IPR000857;    MyTH4_dom.
IPR011993;    PH_like_dom.
IPR001849;    Pleckstrin_homology.
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Pfam
PF00373;    FERM_M;    1.
PF00612;    IQ;    3.
PF00063;    Myosin_head;    1.
PF00784;    MyTH4;    1.
PF00169;    PH;    2.
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SMART
SM00295;    B41;    1.
SM00015;    IQ;    3.
SM00242;    MYSc;    1.
SM00139;    MyTH4;    1.
SM00233;    PH;    2.
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PROSITE
PS00660;    FERM_1;    FALSE_NEG.
PS00661;    FERM_2;    FALSE_NEG.
PS50057;    FERM_3;    1.
PS50096;    IQ;    3.
PS51016;    MYTH4;    1.
PS50003;    PH_DOMAIN;    2.
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PRINTS
PR00193;    MYOSINHEAVY.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   2062       Unconventional myosin-X.
                             /FTId=PRO_0000416244.
DOMAIN       65    727       Myosin head-like.
DOMAIN      742    771       IQ 1.
DOMAIN      765    794       IQ 2.
DOMAIN      788    817       IQ 3.
DOMAIN     1216   1314       PH 1.
DOMAIN     1396   1501       PH 2.
DOMAIN     1551   1699       MyTH4.
DOMAIN     1704   2048       FERM.
COILED      800    936       Potential.
MOD_RES     959    959       Phosphoserine (By similarity).
MOD_RES     961    961       Phosphoserine (By similarity).
MOD_RES     967    967       Phosphoserine (By similarity).
MOD_RES    1011   1011       Phosphoserine (By similarity).
CONFLICT     77     78       GS -> VA (in Ref. 1; CAB56466).
CONFLICT    253    254       QN -> RI (in Ref. 1; CAB56466).
CONFLICT    273    273       G -> E (in Ref. 1; CAB56466).
CONFLICT    501    501       G -> R (in Ref. 1; CAB56466).
CONFLICT    870    870       E -> A (in Ref. 1; CAB56466).
CONFLICT    984    984       N -> S (in Ref. 1; CAB56466).
CONFLICT    993    993       A -> E (in Ref. 1; CAB56466).
CONFLICT   1032   1032       D -> E (in Ref. 1; CAB56466).
CONFLICT   1042   1042       Y -> D (in Ref. 1; CAB56466).
CONFLICT   1083   1083       N -> P (in Ref. 1; CAB56466).
CONFLICT   1371   1371       D -> Y (in Ref. 1; CAB56466).
CONFLICT   1602   1602       D -> H (in Ref. 1; CAB56466).
CONFLICT   1647   1647       R -> L (in Ref. 1; CAB56466).
CONFLICT   1805   1805       S -> G (in Ref. 1; CAB56466).
CONFLICT   1939   1939       N -> T (in Ref. 1; CAB56466).
CONFLICT   1982   1982       D -> E (in Ref. 1; CAB56466).
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Nucleotide Sequence
Length: 6276 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 2062 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
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