SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001009 
UniProt Accession
Theoretical PI
5.46  
Molecular Weight
61226 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Pde1b 
Gene Synonyms/Alias
Pde1b1 
Protein Name
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B 
Protein Synonyms/Alias
Cam-PDE 1BEC=3.1.4.17 63 kDa Cam-PDE; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:15;103333465-103360483;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Calcium/calmodulin-dependent phosphodiesterases (CaM-PDEs) are abundant in testicular cells and in mature spermatozoa and provide one means by which calcium regulates cellular cyclic nucleotide content. The distinct spatial and temporal expression patterns of CaM-PDEs suggest important but different physiological roles for these CaM-PDEs in developing and mature spermatozoa. 
The information of related literatures
1. C. Yan, A. Z. Zhao, W. K. Sonnenburg and J. A. Beavo (2001) Stage and cell-specific expression of calmodulin-dependent phosphodiesterases in mouse testis. Biol Reprod 64(6): 1746-54. 

Abstract
Calcium and cyclic nucleotides are second messengers that regulate the development and functional activity of spermatozoa. Calcium/calmodulin-dependent phosphodiesterases (CaM-PDEs) are abundant in testicular cells and in mature spermatozoa and provide one means by which calcium regulates cellular cyclic nucleotide content. We examined the spatial and temporal expression profiles of three knownCaM-PDE genes, PDE1A, PDE1B, and PDE1C, in the testis. In situ hybridization and immunofluorescent staining showed that both PDE1A and PDE1C are highly expressed but at different stages in developing germ cells. However, a very low hybridization signal of PDE1B exists uniformly throughout the seminiferous epithelium and the interstitium. More specifically, PDE1A mRNA is found in round to elongated spermatids, with protein expression in the tails of elongated and maturing spermatids. In contrast, PDE1C mRNA accumulates during early meiotic prophase and throughout meiotic and postmeiotic stages. Immunocytochemistry showed a diffuse, presumably cytosolic distribution of the expressed protein. The distinct spatial and temporal expression patterns of CaM-PDEs suggest important but different physiological roles for these CaM-PDEs in developing and mature spermatozoa. PMID: [11369604] 

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Figures for illustrating the function of this protein/gene
Ref: C. Yan, A. Z. Zhao, W. K. Sonnenburg and J. A. Beavo (2001) Stage and cell-specific expression of calmodulin-dependent phosphodiesterases in mouse testis. Biol Reprod 64(6): 1746-54. PMID: [11369604]
Ref: C. Yan, A. Z. Zhao, W. K. Sonnenburg and J. A. Beavo (2001) Stage and cell-specific expression of calmodulin-dependent phosphodiesterases in mouse testis. Biol Reprod 64(6): 1746-54. PMID: [11369604]
Ref: C. Yan, A. Z. Zhao, W. K. Sonnenburg and J. A. Beavo (2001) Stage and cell-specific expression of calmodulin-dependent phosphodiesterases in mouse testis. Biol Reprod 64(6): 1746-54. PMID: [11369604]
Ref: C. Yan, A. Z. Zhao, W. K. Sonnenburg and J. A. Beavo (2001) Stage and cell-specific expression of calmodulin-dependent phosphodiesterases in mouse testis. Biol Reprod 64(6): 1746-54. PMID: [11369604]
Ref: C. Yan, A. Z. Zhao, W. K. Sonnenburg and J. A. Beavo (2001) Stage and cell-specific expression of calmodulin-dependent phosphodiesterases in mouse testis. Biol Reprod 64(6): 1746-54. PMID: [11369604]
Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are keyregulators of many important physiological processes. Has apreference for cGMP as a substrate (By similarity). 
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Subcellular Location
Cytoplasm. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell.
GO:0043025 C:neuronal cell body IEA:Compara.
GO:0005634 C:nucleus IEA:Compara.
GO:0004115 F:3',5'-cyclic-AMP phosphodiesterase activity IEA:Compara.
GO:0048101 F:calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity ISO:MGI.
GO:0004117 F:calmodulin-dependent cyclic-nucleotide phosphodiesterase activity ISO:MGI.
GO:0046872 F:metal ion binding IEA:UniProtKB-KW.
GO:0006198 P:cAMP catabolic process IEA:Compara.
GO:0097011 P:cellular response to granulocyte macrophage colony-stimulating factor stimulus IEA:Compara.
GO:0036006 P:cellular response to macrophage colony-stimulating factor stimulus IEA:Compara.
GO:0046069 P:cGMP catabolic process IEA:Compara.
GO:0007626 P:locomotory behavior IMP:MGI.
GO:0030224 P:monocyte differentiation IEA:Compara.
GO:0042053 P:regulation of dopamine metabolic process IMP:MGI.
GO:0001505 P:regulation of neurotransmitter levels IMP:MGI.
GO:0001975 P:response to amphetamine IMP:MGI.
GO:0042428 P:serotonin metabolic process IMP:MGI.
GO:0007165 P:signal transduction IMP:MGI.
GO:0008542 P:visual learning IMP:MGI.
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Interpro
IPR003607;    HD/PDEase_dom.
IPR023088;    PDEase.
IPR002073;    PDEase_catalytic_dom.
IPR023174;    PDEase_CS.
IPR013706;    PDEase_N.
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Pfam
PF00233;    PDEase_I;    1.
PF08499;    PDEase_I_N;    1.
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SMART
SM00471;    HDc;    1.
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PROSITE
PS00126;    PDEASE_I;    1.
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PRINTS
PR00387;    PDIESTERASE1.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    535       Calcium/calmodulin-dependent 3',5'-cyclic
                             nucleotide phosphodiesterase 1B.
                             /FTId=PRO_0000198790.
REGION       27     47       Calmodulin-binding (Potential).
REGION      196    495       Catalytic (By similarity).
ACT_SITE    222    222       Proton donor (By similarity).
METAL       226    226       Divalent metal cation 1 (By similarity).
METAL       262    262       Divalent metal cation 1 (By similarity).
METAL       263    263       Divalent metal cation 1 (By similarity).
METAL       263    263       Divalent metal cation 2 (By similarity).
METAL       369    369       Divalent metal cation 1 (By similarity).
MOD_RES     465    465       Phosphoserine.
CONFLICT     38     38       L -> M (in Ref. 2).
CONFLICT    224    224       Q -> R (in Ref. 3; AAA37367).
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Nucleotide Sequence
Length: 2986 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 535 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
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