SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001093 
UniProt Accession
Theoretical PI
7.14  
Molecular Weight
118560 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gli1 
Gene Synonyms/Alias
Gli 
Protein Name
Zinc finger protein GLI1 
Protein Synonyms/Alias
Glioma-associated oncogene homolog; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:10;126766945-126778645;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. T. L. Kroft, J. Patterson, J. Won Yoon, L. Doglio, D. O. Walterhouse, P. M. Iannaccone and E. Goldberg (2001) GLI1 localization in the germinal epithelial cells alternates between cytoplasm and nucleus. Biol Reprod 65(6): 1663-71. 

Abstract
The zinc finger transcription factor GLI1 is the mediator of signaling by members of the Hedgehog (Hh) family. Male mice in which Desert hedgehog (Dhh), an Hh homologue expressed in Sertoli cells of the testis, was knocked out are sterile, suggesting that the Dhh/GLI1 pathway plays a role in spermatogenesis. Using an antiserum raised against human GLI1, we found that during the first round of spermatogenesis, GLI1 expression is initially cytoplasmic, then shifts to the nuclei of Sertoli and germ cells, and finally shifts back to the cytoplasm. In the adult mouse testis, GLI1 expression localized to the nuclei of germ cells, beginning with pachytene cells and persisting through round spermatids. Localization of GLI1 in elongating spermatids shifted from the nucleus to the cytoplasm and became associated with microtubules. We also examined a line of transgenic mice that overexpressed human GLI1. Male mice in this line were sterile. Spermatogenesis was blocked at the pachytene stage, and a subset of the morphologically indistinguishable pachytene cells underwent apoptosis. Patched-2, which is a Dhh receptor, and Fused, another component of the signal transduction pathway, are expressed in Leydig cells and in primary and secondary spermatocytes. Expression of GLI1 in the same cell types as Patched-2 and Fused and the disruption of spermatogenesis by GLI1 overexpression suggest that GLI1 is the mediator of the Dhh signal in the testis, and that it may be a regulator of spermatogenesis. PMID: [11717126] 

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Figures for illustrating the function of this protein/gene
Ref: T. L. Kroft, J. Patterson, J. Won Yoon, L. Doglio, D. O. Walterhouse, P. M. Iannaccone and E. Goldberg (2001) GLI1 localization in the germinal epithelial cells alternates between cytoplasm and nucleus. Biol Reprod 65(6): 1663-71. PMID: [11717126]
Ref: T. L. Kroft, J. Patterson, J. Won Yoon, L. Doglio, D. O. Walterhouse, P. M. Iannaccone and E. Goldberg (2001) GLI1 localization in the germinal epithelial cells alternates between cytoplasm and nucleus. Biol Reprod 65(6): 1663-71. PMID: [11717126]
Ref: T. L. Kroft, J. Patterson, J. Won Yoon, L. Doglio, D. O. Walterhouse, P. M. Iannaccone and E. Goldberg (2001) GLI1 localization in the germinal epithelial cells alternates between cytoplasm and nucleus. Biol Reprod 65(6): 1663-71. PMID: [11717126]
Ref: T. L. Kroft, J. Patterson, J. Won Yoon, L. Doglio, D. O. Walterhouse, P. M. Iannaccone and E. Goldberg (2001) GLI1 localization in the germinal epithelial cells alternates between cytoplasm and nucleus. Biol Reprod 65(6): 1663-71. PMID: [11717126]
Ref: T. L. Kroft, J. Patterson, J. Won Yoon, L. Doglio, D. O. Walterhouse, P. M. Iannaccone and E. Goldberg (2001) GLI1 localization in the germinal epithelial cells alternates between cytoplasm and nucleus. Biol Reprod 65(6): 1663-71. PMID: [11717126]
Ref: T. L. Kroft, J. Patterson, J. Won Yoon, L. Doglio, D. O. Walterhouse, P. M. Iannaccone and E. Goldberg (2001) GLI1 localization in the germinal epithelial cells alternates between cytoplasm and nucleus. Biol Reprod 65(6): 1663-71. PMID: [11717126]
Function
Acts as a transcriptional activator. May regulate thetranscription of specific genes during normal development. Mayplay a role in craniofacial development and digital development,as well as development of the central nervous system andgastrointestinal tract. Mediates SHH signaling and thus cellproliferation and differentiation (By similarity). 
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Subcellular Location
Cytoplasm (By similarity). Nucleus (Bysimilarity). Note=Tethered in the cytoplasm by binding to SUFU.Activation and translocation to the nucleus is promoted byinteraction with STK36. Phosphorylation by ULK3 may promotenuclear localization. Translocation to the nucleus is promoted byinteraction with ZIC1 (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005929 C:cilium IDA:MGI.
GO:0005737 C:cytoplasm IDA:MGI.
GO:0005634 C:nucleus IDA:MGI.
GO:0003682 F:chromatin binding IDA:MGI.
GO:0003677 F:DNA binding IDA:MGI.
GO:0003705 F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity IDA:MGI.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0030154 P:cell differentiation IEA:UniProtKB-KW.
GO:0021696 P:cerebellar cortex morphogenesis IGI:MGI.
GO:0009953 P:dorsal/ventral pattern formation IGI:MGI.
GO:0030324 P:lung development IGI:MGI.
GO:0060032 P:notochord regression IGI:MGI.
GO:0021983 P:pituitary gland development IGI:MGI.
GO:0045944 P:positive regulation of transcription from RNA polymerase II promoter IDA:MGI.
GO:0009954 P:proximal/distal pattern formation IGI:MGI.
GO:0021938 P:smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation IGI:MGI.
GO:0007283 P:spermatogenesis IDA:MGI.
GO:0007418 P:ventral midline development IGI:MGI.
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Interpro
IPR007087;    Znf_C2H2.
IPR015880;    Znf_C2H2-like.
IPR013087;    Znf_C2H2/integrase_DNA-bd.
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Pfam
PF00096;    zf-C2H2;    1.
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SMART
SM00355;    ZnF_C2H2;    5.
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PROSITE
PS00028;    ZINC_FINGER_C2H2_1;    4.
PS50157;    ZINC_FINGER_C2H2_2;    5.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1111       Zinc finger protein GLI1.
                             /FTId=PRO_0000047198.
ZN_FING     238    263       C2H2-type 1.
ZN_FING     271    298       C2H2-type 2.
ZN_FING     304    328       C2H2-type 3.
ZN_FING     334    359       C2H2-type 4.
ZN_FING     365    390       C2H2-type 5.
COMPBIAS   1042   1059       Asp/Glu-rich (acidic).
MOD_RES     520    520       N6-acetyllysine (By similarity).
CONFLICT    154    154       V -> L (in Ref. 1; AAC09169).
CONFLICT    170    170       H -> Y (in Ref. 1; AAC09169).
CONFLICT    173    173       S -> A (in Ref. 2; BAA85004).
CONFLICT    179    179       T -> I (in Ref. 1; AAC09169).
CONFLICT    194    194       P -> R (in Ref. 1; AAC09169).
CONFLICT    210    210       T -> I (in Ref. 2; BAA85004).
CONFLICT    271    271       F -> S (in Ref. 1; AAC09169).
CONFLICT    474    474       Missing (in Ref. 1; AAC09169).
CONFLICT    567    569       FPP -> LPT (in Ref. 1; AAC09169).
CONFLICT    707    707       E -> D (in Ref. 1; AAC09169).
CONFLICT    777    777       Missing (in Ref. 1; AAC09169).
CONFLICT    864    864       G -> V (in Ref. 1; AAC09169).
CONFLICT    919    920       GL -> RA (in Ref. 1; AAC09169).
CONFLICT    936    936       S -> Y (in Ref. 1; AAC09169).
CONFLICT    947    947       Missing (in Ref. 1; AAC09169).
CONFLICT    951    952       AA -> RR (in Ref. 1; AAC09169).
CONFLICT    967    967       G -> R (in Ref. 1; AAC09169).
CONFLICT    990    990       P -> A (in Ref. 1; AAC09169).
CONFLICT   1029   1029       A -> P (in Ref. 1; AAC09169).
CONFLICT   1062   1063       QG -> R (in Ref. 1; AAC09169).
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Nucleotide Sequence
Length: 3648 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1111 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
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String database  
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