SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001230 
UniProt Accession
Theoretical PI
6.16  
Molecular Weight
235389 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Cit 
Gene Synonyms/Alias
Crik 
Protein Name
Citron Rho-interacting kinase 
Protein Synonyms/Alias
CRIKEC=2.7.11.1 Rho-interacting, serine/threonine-protein kinase 21; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:5;116295287-116458956;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. F. D. Cunto, S. Imarisio, P. Camera, C. Boitani, F. Altruda and L. Silengo (2002) Essential role of citron kinase in cytokinesis of spermatogenic precursors. J Cell Sci 115(Pt 24): 4819-26. 

Abstract
During spermatogenesis, the first morphological indication of spermatogonia differentiation is incomplete cytokinesis, followed by the assembly of stable intercellular cytoplasmic communications. This distinctive feature of differentiating male germ cells has been highly conserved during evolution, suggesting that regulation of the cytokinesis endgame is a crucial aspect of spermatogenesis. However, the molecular mechanisms underlying testis-specific regulation of cytokinesis are still largely unknown. Citron kinase is a myotonin-related protein acting downstream of the GTPase Rho in cytokinesis control. We previously reported that Citron kinase knockout mice are affected by a complex neurological syndrome caused by cytokinesis block and apoptosis of specific neuronal precursors. In this report we show that, in addition, these mice display a dramatic testicular impairment, with embryonic and postnatal loss of undifferentiated germ cells and complete absence of mature spermatocytes. By contrast, the ovaries of mutant females appear essentially normal. Developmental analysis revealed that the cellular depletion observed in mutant testes is caused by increased apoptosis of undifferentiated and differentiating precursors. The same cells display a severe cytokinesis defect, resulting in the production of multinucleated cells and apoptosis. Our data indicate that Citron kinase is specifically required for cytokinesis of the male germ line. PMID: [12432070] 

Back to Top
Figures for illustrating the function of this protein/gene
Function
Plays a role in cytokinesis. Required for KIF14localization to the central spindle and midbody. Probable RHO/RACeffector that binds to the GTP-bound forms of RHO and RAC1. Itprobably binds p21 with a tighter specificity in vivo. Dualspecificity protein kinase activity catalyzing autophosphorylationand phosphorylation of exogenous substrates on bothserine/threonine and tyrosine residues. Plays an important role inthe regulation of cytokinesis and the development of the centralnervous system. Phosphorylates MYL9/MLC2 (By similarity). 
Back to Top
Subcellular Location
Cytoplasm. 
Tissue Specificity
A major signal was observed in testis andbrain, but it was also detected in thymus, spleen, kidney, heartand lung. 
Gene Ontology
GO IDGO termEvidence
GO:0015629 C:actin cytoskeleton IDA:MGI.
GO:0001726 C:ruffle IDA:MGI.
GO:0005773 C:vacuole IDA:MGI.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0046872 F:metal ion binding IEA:UniProtKB-KW.
GO:0005543 F:phospholipid binding IEA:InterPro.
GO:0004674 F:protein serine/threonine kinase activity IDA:UniProtKB.
GO:0005083 F:small GTPase regulator activity IEA:InterPro.
GO:0033205 P:cell cycle cytokinesis ISS:UniProtKB.
GO:0016358 P:dendrite development IMP:MGI.
GO:0035556 P:intracellular signal transduction IEA:InterPro.
GO:0007091 P:mitotic metaphase/anaphase transition IMP:MGI.
GO:0000070 P:mitotic sister chromatid segregation IMP:MGI.
GO:0050774 P:negative regulation of dendrite morphogenesis IDA:MGI.
GO:0045665 P:negative regulation of neuron differentiation ISO:MGI.
GO:0007283 P:spermatogenesis IMP:MGI.
Back to Top
Interpro
IPR000961;    AGC-kinase_C.
IPR001180;    Citron.
IPR017405;    Citron_Rho-interacting_kinase.
IPR011009;    Kinase-like_dom.
IPR011993;    PH_like_dom.
IPR017892;    Pkinase_C.
IPR001849;    Pleckstrin_homology.
IPR002219;    Prot_Kinase_C-like_PE/DAG-bd.
IPR000719;    Prot_kinase_cat_dom.
IPR017441;    Protein_kinase_ATP_BS.
IPR002290;    Ser/Thr_dual-sp_kinase_dom.
IPR008271;    Ser/Thr_kinase_AS.
Back to Top
Pfam
PF00780;    CNH;    1.
PF00069;    Pkinase;    1.
PF00433;    Pkinase_C;    1.
Back to Top
SMART
SM00109;    C1;    1.
SM00036;    CNH;    1.
SM00233;    PH;    1.
SM00133;    S_TK_X;    1.
SM00220;    S_TKc;    1.
Back to Top
PROSITE
PS51285;    AGC_KINASE_CTER;    1.
PS50219;    CNH;    1.
PS50003;    PH_DOMAIN;    1.
PS00107;    PROTEIN_KINASE_ATP;    1.
PS50011;    PROTEIN_KINASE_DOM;    1.
PS00108;    PROTEIN_KINASE_ST;    1.
PS00479;    ZF_DAG_PE_1;    1.
PS50081;    ZF_DAG_PE_2;    1.
Back to Top
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   2055       Citron Rho-interacting kinase.
                             /FTId=PRO_0000085909.
DOMAIN       97    359       Protein kinase.
DOMAIN      360    430       AGC-kinase C-terminal.
DOMAIN     1469   1589       PH.
DOMAIN     1617   1907       CNH.
NP_BIND     103    111       ATP (By similarity).
ZN_FING    1388   1437       Phorbol-ester/DAG-type.
REGION     1132   1328       Interaction with Rho/Rac.
COILED      441   1086       Potential.
COILED     1091   1247       Potential.
COILED     1275   1325       Potential.
MOTIF      1979   1984       SH3-binding (Potential).
COMPBIAS    550    962       Glu-rich.
COMPBIAS   1276   1279       Poly-Lys.
ACT_SITE    221    221       Proton acceptor (By similarity).
BINDING     126    126       ATP (By similarity).
MOD_RES     432    432       Phosphoserine (By similarity).
MOD_RES     439    439       Phosphoserine (By similarity).
MOD_RES     479    479       Phosphoserine.
MOD_RES    1237   1237       Phosphotyrosine.
MOD_RES    1504   1504       Phosphotyrosine.
MOD_RES    1608   1608       Phosphoserine.
MOD_RES    1747   1747       N6-acetyllysine (By similarity).
MOD_RES    1999   1999       Phosphoserine (By similarity).
MOD_RES    2021   2021       Phosphoserine (By similarity).
VAR_SEQ       1    458       Missing (in isoform 3).
                             /FTId=VSP_012436.
VAR_SEQ     459    466       DSQDKCHK -> MLLGEEAM (in isoform 3).
                             /FTId=VSP_012437.
VAR_SEQ     467    494       MEQEMTRLHRRVSEVEAVLSQKEVELKA -> VSISTAGLR
                             PCSRILQSIYAEGSAGGHC (in isoform 2).
                             /FTId=VSP_012438.
VAR_SEQ     495   2055       Missing (in isoform 2).
                             /FTId=VSP_012439.
VAR_SEQ     693    735       Missing (in isoform 4).
                             /FTId=VSP_016093.
VAR_SEQ    1279   1279       K -> KGLFSRRKEDPALPTQ (in isoform 5).
                             /FTId=VSP_016094.
VAR_SEQ    1602   1602       A -> AARDHTSSEHQPVWVE (in isoform 5).
                             /FTId=VSP_016095.
MUTAGEN     126    126       K->A: Loss of phosphorylation.
CONFLICT     78     78       Q -> R (in Ref. 3; BAE26199).
CONFLICT    182    182       F -> L (in Ref. 3; BAE26199).
CONFLICT    234    234       E -> H (in Ref. 3; BAE26199).
CONFLICT   1945   1945       Missing (in Ref. 6; AAH51165).
Back to Top
Nucleotide Sequence
Length: 5019 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 2055 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
YwhabIntAct 
Dlg4IntAct 
Mef2cMINT 
Grin2bIntAct 
Grin1IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Comments