SG ID

SG00001456 

UniProt Accession

F8VQ54_MOUSE;F8VQ54;  

Theoretical PI

FRAGMENT  

Molecular Weight

215769 Da  

Genbank Nucleotide ID

AC124364;  

Genbank Protein ID

 

Gene Name

Dicer1 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Endoribonuclease Dicer 

Mus musculus (Mouse) 

10090 

Temporarily unavailable 

The information of related literatures

1. D. M. Maatouk, K. L. Loveland, M. T. McManus, K. Moore and B. D. Harfe (2008) Dicer1 is required for differentiation of the mouse male germline. Biol Reprod 79(4): 696-703. 

Abstract
MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally regulate gene expression. Hundreds of miRNAs are expressed in mammals; however, their functions are just starting to be uncovered. MicroRNAs are processed from a long hairpin mRNA transcript, down to a approximately 23-nucleotide duplex. The enzyme Dicer1 is required for miRNA processing, and mouse knockouts of Dicer1 are embryonic lethal before 7.5 days postcoitus. To examine the function of miRNAs specifically in the germline, we used a mouse model that expresses Cre recombinase from the TNAP locus and a floxed Dicer1 conditional allele. Removal of Dicer1 from germ cells resulted in male infertility. Germ cells were present in adult testes, but few tubules contained elongating spermatids. Germ cells that did differentiate to elongating spermatids exhibited abnormal morphology and motility. Rarely, sperm lacking Dicer1 could fertilize wild-type eggs to generate viable offspring. These results show that Dicer1 and miRNAs are essential for proper differentiation of the male germline. PMID: [18633141] 

2. M. D. Papaioannou, J. L. Pitetti, S. Ro, C. Park, F. Aubry, O. Schaad, C. E. Vejnar, F. Kuhne, P. Descombes, E. M. Zdobnov, M. T. McManus, F. Guillou, B. D. Harfe, W. Yan, B. Jegou and S. Nef (2009) Sertoli cell Dicer is essential for spermatogenesis in mice. Dev Biol 326(1): 250-9. 

Abstract
Spermatogenesis requires intact, fully competent Sertoli cells. Here, we investigate the functions of Dicer, an RNaseIII endonuclease required for microRNA and small interfering RNA biogenesis, in mouse Sertoli cell function. We show that selective ablation of Dicer in Sertoli cells leads to infertility due to complete absence of spermatozoa and progressive testicular degeneration. The first morphological alterations appear already at postnatal day 5 and correlate with a severe impairment of the prepubertal spermatogenic wave, due to defective Sertoli cell maturation and incapacity to properly support meiosis and spermiogenesis. Importantly, we find several key genes known to be essential for Sertoli cell function to be significantly down-regulated in neonatal testes lacking Dicer in Sertoli cells. Overall, our results reveal novel essential roles played by the Dicer-dependent pathway in mammalian reproductive function, and thus pave the way for new insights into human infertility. PMID: [19071104] 

3. G. J. Kim, I. Georg, H. Scherthan, M. Merkenschlager, F. Guillou, G. Scherer and F. Barrionuevo (2010) Dicer is required for Sertoli cell function and survival. Int J Dev Biol 54(5): 867-75. 

Abstract
Dicer is a key enzyme that processes microRNA precursors into their mature form, enabling them to regulate gene expression. Dicer null mutants die before gastrulation. To study Dicer function in testis development, we crossed mice carrying a conditional Dicer allele with an AMH-Cre transgenic line, thereby inactivating Dicer in Sertoli cells around embryonic day 14.0 (E14.0). Dicer null Sertoli cells show normal embryonic development, and at postnatal day 0 (P0), testis tubules are normal in number and histologically undistinguishable from controls. Subsequently, Dicer-mutant testes show a progressively aberrant development, so that at P6, they contain a reduced number of disorganized testis tubules leading to primary sterility. Apoptosis and prophase I assays reveal a massive wave of apoptosis starting at P3, causing progressive loss of Sertoli cells, but also of germ cells, resulting in drastically reduced testis size. Expression of genes that play crucial roles in testis development, structural integrity and spermatogenesis is downregulated at P0, before morphological changes become apparent, indicating that Dicer-mutant testes are already transcriptionally compromised at this stage. Taken together, the results of this study show that Dicer is required for Sertoli cell function and survival and for spermatogenesis in mice. PMID: [19876815] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0016442	C:RNA-induced silencing complex	IEA:Compara.
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0008026	F:ATP-dependent helicase activity	IEA:InterPro.
GO:0003725	F:double-stranded RNA binding	IEA:Compara.
GO:0035198	F:miRNA binding	IDA:MGI.
GO:0004525	F:ribonuclease III activity	IDA:MGI.
GO:0001525	P:angiogenesis	IMP:MGI.
GO:0006915	P:apoptotic process	IMP:MGI.
GO:0048754	P:branching morphogenesis of a tube	IMP:MGI.
GO:0055013	P:cardiac muscle cell development	IMP:MGI.
GO:0021987	P:cerebral cortex development	IMP:MGI.
GO:0051607	P:defense response to virus	IMP:MGI.
GO:0035116	P:embryonic hindlimb morphogenesis	IMP:MGI.
GO:0071335	P:hair follicle cell proliferation	IMP:MGI.
GO:0031069	P:hair follicle morphogenesis	IMP:MGI.
GO:0060119	P:inner ear receptor cell development	IMP:MGI.
GO:0060576	P:intestinal epithelial cell development	IGI:MGI.
GO:0030324	P:lung development	IMP:MGI.
GO:0035264	P:multicellular organism growth	IMP:MGI.
GO:0043066	P:negative regulation of apoptotic process	IMP:MGI.
GO:0000122	P:negative regulation of transcription from RNA polymerase II promoter	IMP:MGI.
GO:0021889	P:olfactory bulb interneuron differentiation	IMP:MGI.
GO:2000630	P:positive regulation of miRNA metabolic process	IMP:MGI.
GO:0031643	P:positive regulation of myelination	IMP:MGI.
GO:0045944	P:positive regulation of transcription from RNA polymerase II promoter	IMP:MGI.
GO:0009791	P:post-embryonic development	IMP:MGI.
GO:0031054	P:pre-miRNA processing	IDA:MGI.
GO:0030422	P:production of siRNA involved in RNA interference	IDA:MGI.
GO:0051726	P:regulation of cell cycle	IMP:MGI.
GO:0070173	P:regulation of enamel mineralization	IMP:MGI.
GO:0045664	P:regulation of neuron differentiation	IMP:MGI.
GO:0042487	P:regulation of odontogenesis of dentin-containing tooth	IMP:MGI.
GO:0048713	P:regulation of oligodendrocyte differentiation	IMP:MGI.
GO:0045069	P:regulation of viral genome replication	IMP:MGI.
GO:0016075	P:rRNA catabolic process	IEA:InterPro.
GO:0021522	P:spinal cord motor neuron differentiation	IMP:MGI.
GO:0051225	P:spindle assembly	IMP:MGI.
GO:0048536	P:spleen development	IMP:MGI.
GO:0019827	P:stem cell maintenance	IMP:MGI.
GO:0030423	P:targeting of mRNA for destruction involved in RNA interference	IEA:Compara.
GO:0010070	P:zygote asymmetric cell division	IMP:MGI.

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Interpro

IPR005034;    Dicer_dsRNA_binding_fold.
IPR011545;    DNA/RNA_helicase_DEAD/DEAH_N.
IPR001159;    Ds-RNA-bd.
IPR014720;    dsRNA-bd-like.
IPR014001;    Helicase_ATP-bd.
IPR001650;    Helicase_C.
IPR003100;    PAZ.
IPR011907;    RNase_III.
IPR000999;    RNase_III_dom.
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Pfam

PF00270;    DEAD;    1.
PF03368;    dsRNA_bind;    1.
PF00271;    Helicase_C;    1.
PF02170;    PAZ;    1.
PF00636;    Ribonuclease_3;    2.
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SMART

SM00487;    DEXDc;    1.
SM00358;    DSRM;    1.
SM00490;    HELICc;    1.
SM00949;    PAZ;    1.
SM00535;    RIBOc;    2.
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PROSITE

PS51327;    DICER_DSRBF;    1.
PS50137;    DS_RBD;    1.
PS51192;    HELICASE_ATP_BIND_1;    1.
PS51194;    HELICASE_CTER;    1.
PS50821;    PAZ;    1.
PS00517;    RNASE_3_1;    1.
PS50142;    RNASE_3_2;    2.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 177697 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 1906 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Click to view expression 

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