SG00001683

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00001683

UniProt Accession

Theoretical PI

Molecular Weight

Genbank Nucleotide ID

Genbank Protein ID

Gene Name

Gene Synonyms/Alias

Protein Name

Protein Synonyms/Alias

Organism

NCBI Taxonomy ID

Chromosome Location

chr:4;124334889-124337899;1

View in Ensembl genome browser

Function in Stage

premeiotic

Function in Cell Type

spermatogonium

Description

Temporarily unavailable

The information of related literatures

1. X. Wu, J. M. Oatley, M. J. Oatley, A. V. Kaucher, M. R. Avarbock and R. L. Brinster (2010) The POU domain transcription factor POU3F1 is an important intrinsic regulator of GDNF-induced survival and self-renewal of mouse spermatogonial stem cells. Biol Reprod 82(6): 1103-11.

Abstract
Continual spermatogenesis relies on a pool of spermatogonial stem cells (SSCs) that possess the capacity for self-renewal and differentiation. Maintenance of this pool depends on survival of SSCs throughout the lifetime of a male. Response to extrinsic stimulation from glial cell line-derived neurotrophic factor (GDNF), mediated by the PIK3/AKT signaling cascade, is a key pathway of SSC survival. In this study, we found that expression of the POU domain transcription factor POU3F1 in cultured SSCs is up-regulated via this mechanism. Reduction of Pou3f1 gene expression by short interfering RNA (siRNA) treatment induced apoptosis in cultured germ cell populations, and transplantation analyses revealed impaired SSC maintenance in vitro. POU3F1 expression was localized to spermatogonia in cross-sections of prepubertal and adult testes, implying a similar role in vivo. Through comparative analyses, we found that expression of POU5F1, another POU transcription factor implicated as essential for SSC self-renewal, is not regulated by GDNF in cultured SSCs. Transplantation analyses following siRNA treatment showed that POU5F1 expression is not essential for SSC maintenance in vitro. Additionally, expression of NODAL, a putative autocrine regulator of POU5F1 expression in mouse germ cells, could not be detected in SSCs isolated from testes or cultured SSCs. Collectively, these results indicate that POU3F1, but not POU5F1, is an intrinsic regulator of GDNF-induced survival and self-renewal of mouse SSCs. PMID: [20181621]

Figures for illustrating the function of this protein/gene

	Ref: X. Wu, J. M. Oatley, M. J. Oatley, A. V. Kaucher, M. R. Avarbock and R. L. Brinster (2010) The POU domain transcription factor POU3F1 is an important intrinsic regulator of GDNF-induced survival and self-renewal of mouse spermatogonial stem cells. Biol Reprod 82(6): 1103-11. PMID: [20181621]
	Ref: X. Wu, J. M. Oatley, M. J. Oatley, A. V. Kaucher, M. R. Avarbock and R. L. Brinster (2010) The POU domain transcription factor POU3F1 is an important intrinsic regulator of GDNF-induced survival and self-renewal of mouse spermatogonial stem cells. Biol Reprod 82(6): 1103-11. PMID: [20181621]