SG ID

SG00001786 

UniProt Accession

F1LSX8_RAT;F1LSX8;  

Theoretical PI

6.41  

Molecular Weight

133224 Da  

Genbank Nucleotide ID

 

Genbank Protein ID

 

Gene Name

Atp2b4 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Plasma membrane calcium-transporting ATPase 4 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. B. Wilhelm, T. Brandenburger, H. Post and G. Aumuller (2008) Expression and localization of PMCA4 in rat testis and epididymis. Histochem Cell Biol 129(3): 331-43. 

Abstract
It has recently been shown in mice that the plasma membrane Ca(2+)-ATPase isoform 4 (PMCA4) is essential for sperm fertilization capacity. We analyzed whether sperm PMCA4 is formed in the rat during spermatogenesis or is synthesized in the epididymis and transferred onto sperm during sperm maturation. We could show that PMCA4 is conserved in sperm from testis to epididymis. In testis, PMCA4 mRNA was restricted to spermatogonia and early spermatocytes, while the PMCA4 protein was detected in spermatogonia, late spermatocytes, spermatids and in epididymal sperm. In epididymis PMCA4 mRNA was localized in basolateral plasma membranes of epithelial cells of the caput, corpus and cauda epididymidis. In contrast, the protein was only detectable in the epithelial cells of the caput, indicating that PMCA4 mRNA is only translated into protein in caput epithelium. In the epididymal corpus and cauda, PMCA4 mRNA and protein, respectively, was localized and in peritubular cells. Furthermore, we detected an identical distribution of PMCA4a and b splice variants in rat testis, epididymal corpus and cauda. In the caput epididymidis, where PMCA4 is located in the epithelium splice variant 4b was more prominent. Further experiments have to clarify the functional importance of the differences in the PMCA4 distribution. PMID: [18057950] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0016021	C:integral to membrane	IEA:UniProtKB-KW.
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0005388	F:calcium-transporting ATPase activity	IEA:InterPro.
GO:0046872	F:metal ion binding	IEA:InterPro.
GO:0006754	P:ATP biosynthetic process	IEA:InterPro.

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Interpro

IPR022141;    ATP_Ca_trans_C.
IPR023306;    ATPase_cation_domN.
IPR008250;    ATPase_P-typ_ATPase-assoc-dom.
IPR006408;    ATPase_P-typ_Ca-transp_PMCA.
IPR006069;    ATPase_P-typ_cation-exchng_asu.
IPR006068;    ATPase_P-typ_cation-transptr_C.
IPR004014;    ATPase_P-typ_cation-transptr_N.
IPR023300;    ATPase_P-typ_cyto_domA.
IPR023299;    ATPase_P-typ_cyto_domN.
IPR001757;    ATPase_P-typ_ion-transptr.
IPR018303;    ATPase_P-typ_P_site.
IPR023298;    ATPase_P-typ_TM_dom.
IPR005834;    Dehalogen-like_hydro.
IPR023214;    HAD-like_dom.
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Pfam

PF12424;    ATP_Ca_trans_C;    1.
PF00689;    Cation_ATPase_C;    1.
PF00690;    Cation_ATPase_N;    1.
PF00122;    E1-E2_ATPase;    1.
PF00702;    Hydrolase;    1.
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SMART

SM00831;    Cation_ATPase_N;    1.
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PROSITE

PS00154;    ATPASE_E1_E2;    1.
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PRINTS

PR00119;    CATATPASE.;   
PR00121;    NAKATPASE.;   
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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 1205 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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