SG ID

SG00001801 

UniProt Accession

PTTG1_RAT;P97613;  

Theoretical PI

5.33  

Molecular Weight

21571 Da  

Genbank Nucleotide ID

U73030; AF021802;  

Genbank Protein ID

AAB47974.1; AAC40036.1; 

Gene Name

Pttg1 

Gene Synonyms/Alias

Pttg 

Protein Name

Securin 

Protein Synonyms/Alias

Pituitary tumor-transforming gene 1 protein; 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. L. Pei (1999) Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells. J Biol Chem 274(5): 3151-8. 

Abstract
Pituitary tumor-transforming gene (PTTG) is a recently characterized proto-oncogene that is expressed specifically in adult testis. In this study, we have used in situ hybridization and developmental Northern blot assays to demonstrate that PTTG mRNA is expressed stage-specifically in spermatocytes and spermatids during rat spermatogenic cycle. We have used the yeast two-hybrid system to identify proteins that interact with PTTG in testicular cells. Two positive clones were characterized. One of the clones is the ribosomal protein S10, the other encodes a novel human DnaJ homologue designated HSJ2. Northern blot analysis showed that testis contains higher levels of HSJ2 mRNA than other tissues examined, and the expression pattern of HSJ2 mRNA in postnatal rat testis is similar to PTTG. S10 mRNA levels do not vary remarkably among different tissues and remains unchanged during testicular germ cell differentiation. In vitro binding assays demonstrated that both S10 and HSJ2 bind to PTTG specifically and that PTTG can be co-immunoprecipitated with S10 and HSJ2 from transfected cells. Moreover, the binding sites for both proteins were located within the C-terminal 75 amino acids of the PTTG protein. These results suggest that PTTG may play a role in spermatogenesis. PMID: [9915854] 

Figures for illustrating the function of this protein/gene

Function

Regulatory protein, which plays a central role inchromosome stability, in the p53/TP53 pathway, and DNA repair.Probably acts by blocking the action of key proteins. During themitosis, it blocks Separase/ESPL1 function, preventing theproteolysis of the cohesin complex and the subsequent segregationof the chromosomes. At the onset of anaphase, it is ubiquitinated,conducting to its destruction and to the liberation of ESPL1. Itsfunction is however not limited to a blocking activity, since itis required to activate ESPL1. Negatively regulates thetranscriptional activity and related apoptosis activity ofp53/TP53. The negative regulation of p53/TP53 may explain thestrong transforming capability of the protein when it isoverexpressed. May also play a role in DNA repair via itsinteraction with Ku, possibly by connecting DNA damage-responsepathways with sister chromatid separation (By similarity). 

Subcellular Location

Cytoplasm (By similarity). Nucleus (Bysimilarity). 

Tissue Specificity

Expressed at low level in most tissues, exceptin adult testis, where it is highly expressed. Expressed in bothspermatocytes and spermatids. 

Gene Ontology

GO ID	GO term	Evidence
GO:0005737	C:cytoplasm	IEA:UniProtKB-SubCell.
GO:0005634	C:nucleus	IEA:UniProtKB-SubCell.
GO:0004869	F:cysteine-type endopeptidase inhibitor activity	IEA:Compara.
GO:0043022	F:ribosome binding	IPI:RGD.
GO:0051301	P:cell division	IEA:UniProtKB-KW.
GO:0051276	P:chromosome organization	IEA:InterPro.
GO:0006281	P:DNA repair	IEA:UniProtKB-KW.
GO:0045143	P:homologous chromosome segregation	IEA:Compara.
GO:0007067	P:mitosis	IEA:UniProtKB-KW.
GO:0008285	P:negative regulation of cell proliferation	IMP:RGD.
GO:0001558	P:regulation of cell growth	IMP:RGD.

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Interpro

IPR018008;    Securin_separation-inh_met.
IPR006940;    Securin_separation_inhibitor.
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Pfam

PF04856;    Securin;    1.
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SMART

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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 974 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 199 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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