SG ID

SG00001810 

UniProt Accession

F1LNT5_RAT;F1LNT5;  

Theoretical PI

9.21  

Molecular Weight

29941 Da  

Genbank Nucleotide ID

 

Genbank Protein ID

 

Gene Name

Phlda1 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Pleckstrin homology-like domain family A member 1 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. F. Liu, Z. L. Xu, X. J. Qian, W. Y. Qiu and H. Huang (2011) Expression of Hsf1, Hsf2, and Phlda1 in cells undergoing cryptorchid-induced apoptosis in rat testes. Mol Reprod Dev 78(4): 283-91. 

Abstract
Surgery-induced cryptorchidism, in which the testes are prevented from descending into the scrotal sac, results in testicular germ cell death, and it is commonly used as an experimental tool in the study of spermatogenesis. However, the molecular events underlying the activation of germ cell death remain poorly understood. In the present study, we investigate selective cell loss from cryptorchid rat testis by using DNA flow cytometry and by determining protein and mRNA expression of Hsf1, Hsf2, and Phlda1. The hypo-haploid cell fraction is significantly decreased as early as 3 days after surgical induction of cryptorchidism (from 42.01 +/- 5.74% to 15.98 +/- 3.88%), followed by a significant decrease in the haploid cell fraction at Day 7. At the latter time point, an apoptotic peak of spermatocytes appears in DNA histograms just before the tetraploid peak; the percentage of aneuploid cells between diploid and tetraploid rises as high as 14.05 +/- 2.98% of the total cells in 7-day cryptorchid testis, suggesting that a large number of spermatocytes are undergoing apoptosis. The expression of Phlda1 mRNA is significantly elevated 3 days after induction of cryptorchidism. After 7 days of cryptorchidism, Hsf1 and Phlda1 are strongly expressed in the nucleus and cytoplasm, respectively, of primary spermatocytes. Numerous apoptotic spermatocytes are also observed at this time point. These results suggest that the Hsf1/Phlda1 pathway plays an important role in the apoptosis of primary spermatocytes in cryptorchid testis. We present evidence suggesting that Hsf2 is also involved in germ cell removal in cryptorchid testis. PMID: [21480429] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005634	C:nucleus	IEA:Compara.
GO:0005886	C:plasma membrane	IEA:Compara.
GO:0005543	F:phospholipid binding	IEA:InterPro.
GO:0045210	P:FasL biosynthetic process	IEA:Compara.

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Interpro

IPR011993;    PH_like_dom.
IPR001849;    Pleckstrin_homology.
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Pfam

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SMART

SM00233;    PH;    1.
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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 262 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

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