SG ID

SG00001816 

UniProt Accession

SPDYA_RAT;Q8R496;Q68G34;  

Theoretical PI

9  

Molecular Weight

36223 Da  

Genbank Nucleotide ID

AF492385; BC078729;  

Genbank Protein ID

AAM09568.1; AAH78729.1; 

Gene Name

Spdya 

Gene Synonyms/Alias

Spdy1 

Protein Name

Speedy protein A 

Protein Synonyms/Alias

GS4; Rapid inducer of G2/M progression in oocytes A;RINGO A Speedy-1;Spy1 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. M. L. Liu, K. Y. Pei, X. Q. Shi, D. Y. Liu and M. C. Jia (2007) Identification and characterization of a novel spermatogenesis related gene LM23 in rat testis. Biochem Biophys Res Commun 356(3): 576-81. 

Abstract
The aim of this study is to screen the novel gene related to the spermatogenesis. A novel rat testis-specific gene LM23 was identified and characterized by differential display PCR with total RNA from rat type A spermatogonia, pachytene spermatocytes, and round spermatids. LM23 cDNA consists of 1896 base pairs (bp) with a complete open reading frame of 936 bp, and encodes a putative protein including 312 amino acids, which shares no significant homology with any known gene. The sequence of LM23 was submitted to GenBank and the Accession No. was AF492385. Multitissue Northern blot and RT-PCR analysis showed LM23 was specific expression in testis, while its expression was not detected in other tissues. Real-time PCR analysis showed that the expression level of LM23 was highest in spermatocytes and very low in spermatogonia. In situ hybridization revealed strong cytoplasmic positive signal in spermatocytes and weak signal in spermatids and spermatogonia. These results indicated LM23 possessed the testis-specific and stage-specific expression characteristics, and possibly involved in rat spermatogenesis. PMID: [17376406] 

2. Y. M. Cheng, M. L. Liu and M. C. Jia (2011) LM23 is a novel member of the Speedy/Ringo family at the crossroads of life and death of spermatogenic cell. Asian J Androl 13(3): 446-52. 

Abstract
LM23 is a gene specifically expressed in the testis of Rattus norvegicus, as previously reported by our laboratory. The aim of the study is to further investigate the biological function of LM23. Several bioinformatic tools were utilized, including PROSITE and BLAST. To determine the subcellullar localization of LM23, a polyclonal antibody specific for LM23 was generated via the immunization of rabbits. The LM23 gene was cloned from rat testis tissue, and LM23 protein was expressed in Escherichia coli. The biological function of LM23 was analyzed with microarray analysis and immunohistochemistry, using a rat model of LM23 gene knockdown. The results suggested that LM23 belongs to the Speedy/Ringo family. LM23 regulated the G/S and G/M transitions of the cell cycle during spermatogenesis. Downregulation of the LM23 gene during spermatogenesis could lead to the activation of both the Fas-FasL pathway and the mitochondrial pathway. These novel findings indicate that LM23 has a diverse array of functions that are important in both the life and death of the spermatogenic cell. PMID: [21540869] 

3. M. L. Liu, Y. M. Cheng and M. C. Jia (2010) LM23 is essential for spermatogenesis in Rattus norvegicus. Front Biosci (Elite Ed) 2(): 187-94. 

Abstract
LM23 is a gene with testis-specific expression in Rattus norvegicus. To reveal the function of LM23 in the testis, we used lentivirus-mediated RNA interference (RNAi) to knock down LM23 expression in a tissue-specific manner in vivo. A lentiviral vector expressing a short hairpin RNA (shRNA) targeting LM23 was microinjected into the efferent ducts of R. norvegicus testes. The expression of LM23 in the treated testes was significantly knocked down compared with controls. These LM23-shRNA testes contained germ cells arrested at the spermatocyte stage, and showed increased apoptosis and disregulation of some meiotic genes. The results demonstrate the validity of the RNAi approach for targeting LM23 and reveal that LM23 expression in the testis is crucial for meiosis during spermatogenesis in R. norvegicus. PMID: [20036869] 

Figures for illustrating the function of this protein/gene

Function

Regulates the G1/S phase transition of the cell cycle bybinding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activateCDK2 without promoting CDK2 phosphorylation. Mediates cellsurvival during the DNA damage process through activation of CDK2(By similarity). 

Subcellular Location

Nucleus (By similarity). 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005634	C:nucleus	ISS:UniProtKB.
GO:0000082	P:G1/S transition of mitotic cell cycle	ISS:UniProtKB.
GO:0007275	P:multicellular organismal development	IEA:UniProtKB-KW.
GO:0008284	P:positive regulation of cell proliferation	IEA:Compara.
GO:0045737	P:positive regulation of cyclin-dependent protein kinase activity	ISS:UniProtKB.
GO:0006974	P:response to DNA damage stimulus	IEA:UniProtKB-KW.

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Interpro

IPR020984;    Cell_cycle_regulatory_Spy1.
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Pfam

PF11357;    Spy1;    1.
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SMART

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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 1896 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 312 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt	Gene Symbol	Ref Databases
CDK1_RAT	Cdk1	String
EMB_RAT	Emb	String
CDK1_RAT	Cdk1	String
EMB_RAT	Emb	String
UBC_RAT	Ubc	String
UBC_RAT	Ubc	String

View Microarray data

Temporarily unavailable 

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