SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001831 
UniProt Accession
Theoretical PI
5.3  
Molecular Weight
35608 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Ppp2ca 
Gene Synonyms/Alias
 
Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform 
Protein Synonyms/Alias
PP2A-alphaEC=3.1.3.16 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:10;37621256-37641006;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. R. van den Ham, F. M. van Dissel-Emiliani and A. M. van Pelt (2003) Expression of the scaffolding subunit A of protein phosphatase 2A during rat testicular development. Biol Reprod 68(4): 1369-75. 

Abstract
Previously, we found that the poly(A)+ RNA of the scaffolding subunit A (alpha isoform) of protein phosphatase 2A (PP2A-Aalpha) was clearly expressed by fetal gonocytes but weakly expressed by adult single (As), paired (Apr), and aligned (Aal) A spermatogonia. The scaffolding subunit A of PP2A (PP2A-A) is the major subunit in the formation of a functional PP2A holoenzyme. In this study, we investigated the expression of PP2A-A during testicular development in more detail using in situ hybridization, immunohistochemistry, and Western blot with testes of rats of various ages from 16 days postcoitum (pc) to adulthood. The expression of PP2A-A was detected in fetal proliferative gonocytes at 16 days pc, declining thereafter during the quiescent period of the gonocytes. From the day of birth to the start of spermatogenesis (Day 4 postpartum [pp]), the number of PP2A-A-immunopositive gonocytes increased. At Day 4 pp, the first A1 spermatogonia appeared along the basement membrane; all were PP2A-A positive. In the adult, PP2A-A was upregulated during the differentiation of the As, Apr, and Aal spermatogonia to the A1 spermatogonia and expressed thereafter by all other spermatogonia. Spermatocytes from the pachytene stage onward and all spermatids in the adult testis also showed clear expression of PP2A-A. In Sertoli cells, PP2A-A was detected during their proliferative period at 19 days pc to 15 days pp. The presence of a functional enzyme was confirmed by the additional detection of the catalytic subunit C of PP2A using Western blot analyses at various ages during testicular development. This apparent pattern of expression of PP2A-A during testicular development suggests that PP2A may play an important role in the proliferation of distinct populations of testicular cells and during meiosis and sperm maturation. PMID: [12606433] 

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Figures for illustrating the function of this protein/gene
Ref: R. van den Ham, F. M. van Dissel-Emiliani and A. M. van Pelt (2003) Expression of the scaffolding subunit A of protein phosphatase 2A during rat testicular development. Biol Reprod 68(4): 1369-75. PMID: [12606433]
Ref: R. van den Ham, F. M. van Dissel-Emiliani and A. M. van Pelt (2003) Expression of the scaffolding subunit A of protein phosphatase 2A during rat testicular development. Biol Reprod 68(4): 1369-75. PMID: [12606433]
Ref: R. van den Ham, F. M. van Dissel-Emiliani and A. M. van Pelt (2003) Expression of the scaffolding subunit A of protein phosphatase 2A during rat testicular development. Biol Reprod 68(4): 1369-75. PMID: [12606433]
Function
PP2A is the major phosphatase for microtubule-associatedproteins (MAPs). PP2A can modulate the activity of phosphorylase Bkinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2kinase. Cooperates with SGOL2 to protect centromeric cohesin fromseparase-mediated cleavage in oocytes specifically during meiosisI. Activates RAF1 by dephosphorylating it at 'Ser-259' (Bysimilarity). 
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Subcellular Location
Cytoplasm (By similarity). Nucleus (Bysimilarity). Chromosome, centromere (By similarity). Cytoplasm,cytoskeleton, spindle pole (By similarity). Note=In prometaphasecells, but not in anaphase cells, localizes at centromeres. Duringmitosis, also found at spindle poles. Centromeric localizationrequires the presence of SGOL2 (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0000775 C:chromosome, centromeric region IEA:UniProtKB-SubCell.
GO:0005829 C:cytosol IEA:Compara.
GO:0005634 C:nucleus IEA:UniProtKB-SubCell.
GO:0005886 C:plasma membrane IEA:Compara.
GO:0000159 C:protein phosphatase type 2A complex IEA:Compara.
GO:0000922 C:spindle pole IEA:UniProtKB-SubCell.
GO:0046872 F:metal ion binding IEA:UniProtKB-KW.
GO:0004722 F:protein serine/threonine phosphatase activity IDA:RGD.
GO:0007126 P:meiosis IEA:UniProtKB-KW.
GO:0007498 P:mesoderm development IEA:Compara.
GO:0010719 P:negative regulation of epithelial to mesenchymal transition IEA:Compara.
GO:0071902 P:positive regulation of protein serine/threonine kinase activity IEA:Compara.
GO:0006470 P:protein dephosphorylation IDA:RGD.
GO:0070208 P:protein heterotrimerization IMP:MGI.
GO:0031952 P:regulation of protein autophosphorylation IMP:MGI.
GO:0042176 P:regulation of protein catabolic process IMP:MGI.
GO:0010469 P:regulation of receptor activity IMP:MGI.
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Interpro
IPR004843;    Metallo_PEstase_dom.
IPR006186;    Ser/Thr-sp_prot-phosphatase.
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Pfam
PF00149;    Metallophos;    1.
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SMART
SM00156;    PP2Ac;    1.
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PROSITE
PS00125;    SER_THR_PHOSPHATASE;    1.
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PRINTS
PR00114;    STPHPHTASE.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    309       Serine/threonine-protein phosphatase 2A
                             catalytic subunit alpha isoform.
                             /FTId=PRO_0000058843.
ACT_SITE    118    118       Proton donor (By similarity).
METAL        57     57       Iron (By similarity).
METAL        59     59       Iron (By similarity).
METAL        85     85       Iron (By similarity).
METAL        85     85       Manganese (By similarity).
METAL       117    117       Manganese (By similarity).
METAL       167    167       Manganese (By similarity).
METAL       241    241       Manganese (By similarity).
MOD_RES       4      4       N6-acetyllysine (By similarity).
MOD_RES     307    307       Phosphotyrosine (By similarity).
MOD_RES     309    309       Leucine methyl ester (By similarity).
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Nucleotide Sequence
Length: 1738 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 309 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
PPP2R5BMINT 
PPP2R2AMINT 
_MINT 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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