SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001861 
UniProt Accession
Theoretical PI
4.93  
Molecular Weight
84815 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Hsp90aa1 
Gene Synonyms/Alias
Hsp86, Hspca 
Protein Name
Heat shock protein HSP 90-alpha 
Protein Synonyms/Alias
Heat shock 86 kDa;HSP 86HSP86 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:6;135413623-135419147;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. S. Ohsako, D. Bunick and Y. Hayashi (1995) Immunocytochemical observation of the 90 KD heat shock protein (HSP90). J Histochem Cytochem 43(1): 67-76. 

Abstract
We used immunocytochemistry to detect the 90 KD major heat shock protein (HSP90), a potential regulator of gene expression, during male and female rat gonad development. In the Day 13.0 post-coital (dpc) fetal gonad, strong immunoreactivity to anti-HSP90 antibody was shown in the cytoplasm of primordial germ cells (PGCs). Other somatic cells in the gonad showed only faint reactivity. During testicular development, strong immunostaining was observed in the cytoplasm of embryonic germ cells and in spermatogonia and spermatocytes of the pre-pubertal testis. In adult testis reactivity of spermatogonia and pachytene spermatocytes was strong but reactivity of post-meiotic spermatogenic cells, i.e., secondary spermatocytes and spermatids, was extremely reduced. During ovarian development, immunostaining was also observed in the oogonia and the oocytes of pre-pubertal ovary. However, the staining of oocytes was reduced with the development of primordial follicles during the first week after birth. This study revealed that HSP90 is highly expressed in PGCs and continues to be expressed in both male and female pre-meiotic germ cells. The HSP90 accumulation may be essential for both male and female mammalian pre-meiotic germ cells. PMID: [7822767] 

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Figures for illustrating the function of this protein/gene
Function
Molecular chaperone that promotes the maturation,structural maintenance and proper regulation of specific targetproteins involved for instance in cell cycle control and signaltransduction. Undergoes a functional cycle that is linked to itsATPase activity. This cycle probably induces conformationalchanges in the client proteins, thereby causing their activation.Interacts dynamically with various co-chaperones that modulate itssubstrate recognition, ATPase cycle and chaperone function (Bysimilarity). 
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Subcellular Location
Cytoplasm (By similarity). Melanosome (Bysimilarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0016324 C:apical plasma membrane IDA:RGD.
GO:0016323 C:basolateral plasma membrane IDA:RGD.
GO:0031526 C:brush border membrane IDA:RGD.
GO:0009986 C:cell surface IDA:RGD.
GO:0005829 C:cytosol IDA:RGD.
GO:0031012 C:extracellular matrix IDA:RGD.
GO:0042470 C:melanosome IEA:UniProtKB-SubCell.
GO:0043005 C:neuron projection IDA:RGD.
GO:0043025 C:neuronal cell body IDA:RGD.
GO:0048471 C:perinuclear region of cytoplasm IDA:RGD.
GO:0043234 C:protein complex IDA:RGD.
GO:0005625 C:soluble fraction IDA:RGD.
GO:0005524 F:ATP binding IDA:RGD.
GO:0002135 F:CTP binding IDA:RGD.
GO:0032564 F:dATP binding IDA:RGD.
GO:0005525 F:GTP binding IDA:RGD.
GO:0003729 F:mRNA binding IDA:RGD.
GO:0030235 F:nitric-oxide synthase regulator activity ISS:UniProtKB.
GO:0002134 F:UTP binding IDA:RGD.
GO:0010659 P:cardiac muscle cell apoptotic process IEP:RGD.
GO:0001764 P:neuron migration IMP:RGD.
GO:0060452 P:positive regulation of cardiac muscle contraction IEP:RGD.
GO:0045793 P:positive regulation of cell size IMP:RGD.
GO:0010592 P:positive regulation of lamellipodium assembly IMP:RGD.
GO:0045429 P:positive regulation of nitric oxide biosynthetic process ISS:UniProtKB.
GO:0033160 P:positive regulation of protein import into nucleus, translocation IMP:RGD.
GO:0006457 P:protein folding TAS:RGD.
GO:0043627 P:response to estrogen stimulus IEP:RGD.
GO:0009408 P:response to heat IEP:RGD.
GO:0009651 P:response to salt stress IEP:RGD.
GO:0003009 P:skeletal muscle contraction IEP:RGD.
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Interpro
IPR003594;    ATPase-like_ATP-bd.
IPR019805;    Heat_shock_protein_90_CS.
IPR001404;    Hsp90.
IPR020575;    Hsp90_N.
IPR020568;    Ribosomal_S5_D2-typ_fold.
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Pfam
PF02518;    HATPase_c;    1.
PF00183;    HSP90;    1.
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SMART
SM00387;    HATPase_c;    1.
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PROSITE
PS00298;    HSP90;    1.
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PRINTS
PR00775;    HEATSHOCK90.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
INIT_MET      1      1       Removed.
CHAIN         2    733       Heat shock protein HSP 90-alpha.
                             /FTId=PRO_0000062915.
REGION      683    733       Required for homodimerization (By
                             similarity).
MOTIF       729    733       TPR repeat-binding.
BINDING      51     51       ATP (By similarity).
BINDING      93     93       ATP (By similarity).
BINDING     112    112       ATP (By similarity).
BINDING     138    138       ATP; via amide nitrogen (By similarity).
BINDING     401    401       ATP (By similarity).
MOD_RES       5      5       Phosphothreonine; by PRKDC (By
                             similarity).
MOD_RES       7      7       Phosphothreonine; by PRKDC (By
                             similarity).
MOD_RES     224    224       N6-acetyllysine (By similarity).
MOD_RES     231    231       Phosphoserine (By similarity).
MOD_RES     252    252       Phosphoserine (By similarity).
MOD_RES     263    263       Phosphoserine (By similarity).
MOD_RES     314    314       Phosphotyrosine (By similarity).
MOD_RES     400    400       Phosphoserine (By similarity).
MOD_RES     411    411       N6-acetyllysine (By similarity).
MOD_RES     444    444       N6-acetyllysine (By similarity).
MOD_RES     459    459       N6-acetyllysine (By similarity).
MOD_RES     490    490       N6-acetyllysine (By similarity).
MOD_RES     493    493       Phosphotyrosine (By similarity).
MOD_RES     577    577       N6-acetyllysine (By similarity).
MOD_RES     586    586       N6-acetyllysine (By similarity).
MOD_RES     599    599       S-nitrosocysteine (By similarity).
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Nucleotide Sequence
Length: 6639 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 733 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Cyp2b1BioGRID 
Cyp1a1BioGRID 
Cyp2e1BioGRID 
Slc2a4IntAct 
Mapk3MINT 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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