SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001891 
UniProt Accession
Theoretical PI
5.77  
Molecular Weight
57567 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Pparg 
Gene Synonyms/Alias
Nr1c3 
Protein Name
Peroxisome proliferator-activated receptor gamma 
Protein Synonyms/Alias
PPAR-gamma Nuclear receptor subfamily 1 group C member 3; 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:4;151493447-151617331;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. K. Thomas, D. Y. Sung, X. Chen, W. Thompson, Y. E. Chen, J. McCarrey, W. Walker and M. Griswold (2011) Developmental patterns of PPAR and RXR gene expression during spermatogenesis. Front Biosci (Elite Ed) 3(): 1209-20. 

Abstract
Members of the family of nuclear receptors that include peroxisome proliferator-activated receptors (PPARs) and retinoid X receptors (RXRs) are important mediators of selective gene activation during development and cellular differentiation. In this study, developmentally-specific PPAR and RXR patterns of expression that occur in somatic and germ cell populations in the testis were determined using quantative real-time PCR (qRT-PCR) studies on RNAs that were isolated from StaPut purified mouse germ cells and primary rat Sertoli cells. These qRT-PCR studies indicate that transcripts encoding the PPAR-Alpha (alpha), -Beta (beta), and -Gamma (gamma) and RXR -Alpha (alpha), -Beta (beta), and -Gamma (gamma) are developmentally expressed in both differentiating germ and Sertoli cells. In further experiments aimed at deciphering the physiological role that PPAR-Gamma (gamma) plays in Sertoli cells, 15-day primary rat Sertoli cells were infected with recombinant adenoviral vectors containing PPAR-Gamma (gamma) cDNA and PPAR-Gamma (gamma) RNAi constructs. Affymetrix microarray analysis and qRT-PCR validation studies using total RNA isolated from these transfected cells indicated that PPAR-Gamma regulates the pattern of expression of key lipid metabolic genes in Sertoli cells. PMID: [21622127] 

Back to Top
Figures for illustrating the function of this protein/gene
Function
Receptor that binds peroxisome proliferators such ashypolipidemic drugs and fatty acids. Once activated by a ligand,the receptor binds to a promoter element in the gene for acyl-CoAoxidase and activates its transcription. It therefore controls theperoxisomal beta-oxidation pathway of fatty acids. Key regulatorof adipocyte differentiation and glucose homeostasis. 
Back to Top
Subcellular Location
Nucleus (By similarity). Cytoplasm (Bysimilarity). Note=Redistributed from the nucleus to the cytosolthrough a MAP2K1/MEK1-dependent manner (By similarity). 
Tissue Specificity
Highest expression in adipose tissue. 
Gene Ontology
GO IDGO termEvidence
GO:0005829 C:cytosol IDA:RGD.
GO:0005634 C:nucleus IDA:RGD.
GO:0003682 F:chromatin binding IEA:Compara.
GO:0008144 F:drug binding IEA:Compara.
GO:0004879 F:ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity IEA:InterPro.
GO:0001012 F:RNA polymerase II regulatory region DNA binding IEA:Compara.
GO:0043565 F:sequence-specific DNA binding IEA:InterPro.
GO:0003700 F:sequence-specific DNA binding transcription factor activity IDA:BHF-UCL.
GO:0003707 F:steroid hormone receptor activity IEA:InterPro.
GO:0044212 F:transcription regulatory region DNA binding IDA:BHF-UCL.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0006919 P:activation of cysteine-type endopeptidase activity involved in apoptotic process IEA:Compara.
GO:0050873 P:brown fat cell differentiation IEA:Compara.
GO:0045165 P:cell fate commitment IEA:Compara.
GO:0048469 P:cell maturation IEA:Compara.
GO:0032869 P:cellular response to insulin stimulus IEA:Compara.
GO:0071285 P:cellular response to lithium ion IEA:Compara.
GO:0071407 P:cellular response to organic cyclic compound IEA:Compara.
GO:0030855 P:epithelial cell differentiation IEA:Compara.
GO:0019395 P:fatty acid oxidation IMP:RGD.
GO:0042593 P:glucose homeostasis IEA:Compara.
GO:0007507 P:heart development IEP:RGD.
GO:0006917 P:induction of apoptosis IEA:Compara.
GO:0042953 P:lipoprotein transport IEA:Compara.
GO:0015909 P:long-chain fatty acid transport IEA:Compara.
GO:0045713 P:low-density lipoprotein particle receptor biosynthetic process IEA:Compara.
GO:0030224 P:monocyte differentiation IEA:Compara.
GO:0002674 P:negative regulation of acute inflammatory response IMP:RGD.
GO:0030308 P:negative regulation of cell growth IMP:RGD.
GO:0008285 P:negative regulation of cell proliferation IMP:RGD.
GO:0010887 P:negative regulation of cholesterol storage IEA:Compara.
GO:0060336 P:negative regulation of interferon-gamma-mediated signaling pathway IEA:Compara.
GO:0010871 P:negative regulation of receptor biosynthetic process IEA:Compara.
GO:0010891 P:negative regulation of sequestering of triglyceride IEA:Compara.
GO:0051974 P:negative regulation of telomerase activity IMP:RGD.
GO:0000122 P:negative regulation of transcription from RNA polymerase II promoter IEA:Compara.
GO:0031100 P:organ regeneration IEP:RGD.
GO:0001890 P:placenta development IEA:Compara.
GO:0045600 P:positive regulation of fat cell differentiation ISS:HGNC.
GO:0046321 P:positive regulation of fatty acid oxidation IMP:RGD.
GO:0048714 P:positive regulation of oligodendrocyte differentiation IMP:RGD.
GO:0051091 P:positive regulation of sequence-specific DNA binding transcription factor activity IEA:Compara.
GO:0045944 P:positive regulation of transcription from RNA polymerase II promoter IDA:BHF-UCL.
GO:0008217 P:regulation of blood pressure IEA:Compara.
GO:0031000 P:response to caffeine IEP:RGD.
GO:0009409 P:response to cold IEP:RGD.
GO:0042493 P:response to drug IEP:RGD.
GO:0043627 P:response to estrogen stimulus IEP:RGD.
GO:0055098 P:response to low-density lipoprotein particle stimulus IEA:Compara.
GO:0033189 P:response to vitamin A IEP:RGD.
GO:0050872 P:white fat cell differentiation ISS:HGNC.
Back to Top
Interpro
IPR003074;    1Cnucl_rcpt.
IPR003077;    1Cnucl_rcpt_G.
IPR008946;    Nucl_hormone_rcpt_ligand-bd.
IPR000536;    Nucl_hrmn_rcpt_lig-bd_core.
IPR022590;    PPARgamma_N.
IPR001723;    Str_hrmn_rcpt.
IPR001628;    Znf_hrmn_rcpt.
IPR013088;    Znf_NHR/GATA.
Back to Top
Pfam
PF00104;    Hormone_recep;    1.
PF12577;    PPARgamma_N;    1.
PF00105;    zf-C4;    1.
Back to Top
SMART
SM00430;    HOLI;    1.
SM00399;    ZnF_C4;    1.
Back to Top
PROSITE
PS00031;    NUCLEAR_REC_DBD_1;    1.
PS51030;    NUCLEAR_REC_DBD_2;    1.
Back to Top
PRINTS
PR01288;    PROXISOMEPAR.;   
PR01291;    PROXISOMPAGR.;   
PR00398;    STRDHORMONER.;   
PR00047;    STROIDFINGER.;   
Back to Top
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    505       Peroxisome proliferator-activated
                             receptor gamma.
                             /FTId=PRO_0000053497.
DNA_BIND    136    210       Nuclear receptor.
ZN_FING     139    159       NR C4-type.
ZN_FING     176    198       NR C4-type.
REGION      205    280       Interaction with FAM120B (By similarity).
REGION      317    505       Ligand-binding (By similarity).
MOD_RES     112    112       Phosphoserine; by MAPK.
VAR_SEQ       1     30       Missing (in isoform 1).
                             /FTId=VSP_003649.
MUTAGEN     112    112       S->A: Increases adipogenic activity.
CONFLICT    111    111       A -> R (in Ref. 3; CAA73382).
Back to Top
Nucleotide Sequence
Length: 1785 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 505 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
Comments