SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001896 
UniProt Accession
Theoretical PI
7.75  
Molecular Weight
27109 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Fstl3 
Gene Synonyms/Alias
Flrg 
Protein Name
Follistatin-related protein 3 
Protein Synonyms/Alias
Follistatin-like protein 3; Follistatin-related gene protein;Flags: Precursor 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:7;11437266-11442906;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. Y. Ohsawa, G. Zhang, S. Kametaka, M. Shibata, M. Koike, S. Waguri and Y. Uchiyama (2003) Purification, cDNA cloning, and secretory properties of FLRG protein from PC12 cells and the distribution of FLRG mRNA and protein in rat tissues. Arch Histol Cytol 66(4): 367-81. 

Abstract
A 35 kD protein was isolated and purified from conditioned media of Bcl-2 cDNA-transfected PC12 cells and its cDNA cloned. A database analysis showed that the 35 kD protein is a rat homologue of the human FLRG protein. The biochemical as well as morphological properties of the rat FLRG protein in PC12 cells were examined and its distribution in rat tissues determined. The levels of FLRG mRNA expressed were low during the fetal period, compared with those of follistatin mRNA. The distribution of FLRG and follistatin mRNAs differed from each other after birth; the expression levels of FLRG mRNA were abundant in the adrenal gland and testis, whereas those of follistatin mRNA and activin A were markedly high in the ovary. The presence of FLRG mRNA and/or protein was confirmed in spermatocytes at various differentiating stages andin endocrine cells of both the adrenal cortex and medulla. When overexpressed in PC12 cells, the FLRG protein was found to be stored in secretory granules of the cells and largely secreted by a regulated pathway, while activin A enhancedthe constitutive secretion of the FLRG protein from wild-typpe PC12 cells, indicating that the FLRG protein possesses dualproperties in secretory pathways. The different distribution between FLRG and follistatin mRNA suggests that, like follistatin in the ovary, the FLRG protein may be involved in the maintenance of spermatogenesis in the testis and the growth and function of adrenal tissue cells, probably by regulating the functions of its binding partners such as the TGF-beta ( superfamily members. PMID: [14692692] 

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Figures for illustrating the function of this protein/gene
Function
The secreted form is a binding and antagonizing proteinfor members of the TGF-beta family, such us activin, BMP2 andMSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-inducedcellular signaling; more effective on activin A than on activin B.Involved in bone formation; inhibits osteoclast differentiation.Involved in hematopoiesis; involved in differentiation ofhemopoietic progenitor cells, increases hematopoietic celladhesion to fibronectin and seems to contribute to the adhesion ofhematopoietic precursor cells to the bone marrow stroma. Thenuclear form is probably involved in transcriptional regulationvia interaction with MLLT10 (By similarity). 
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Subcellular Location
Secreted (By similarity). Nucleus (Bysimilarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005615 C:extracellular space IDA:RGD.
GO:0005794 C:Golgi apparatus IDA:RGD.
GO:0044306 C:neuron projection terminus IDA:RGD.
GO:0005634 C:nucleus IEA:UniProtKB-SubCell.
GO:0030141 C:secretory granule IDA:RGD.
GO:0030325 P:adrenal gland development IEP:RGD.
GO:0071248 P:cellular response to metal ion IEP:RGD.
GO:0002244 P:hemopoietic progenitor cell differentiation IEA:Compara.
GO:0001822 P:kidney development IEP:RGD.
GO:0030324 P:lung development IEP:RGD.
GO:0008584 P:male gonad development IEP:RGD.
GO:0032926 P:negative regulation of activin receptor signaling pathway IEA:Compara.
GO:0030514 P:negative regulation of BMP signaling pathway IEA:Compara.
GO:0045671 P:negative regulation of osteoclast differentiation IEA:Compara.
GO:0001503 P:ossification IEA:UniProtKB-KW.
GO:0022409 P:positive regulation of cell-cell adhesion IEA:Compara.
GO:0045944 P:positive regulation of transcription from RNA polymerase II promoter IEA:Compara.
GO:0007283 P:spermatogenesis IEP:RGD.
GO:0006351 P:transcription, DNA-dependent IEA:UniProtKB-KW.
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Interpro
IPR003645;    Fol_N.
IPR015369;    Follistatin/Osteonectin_EGF.
IPR011497;    Kazal-type_dom.
IPR002350;    Prot_inh_Kazal.
IPR017878;    TB_dom.
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Pfam
PF09289;    FOLN;    1.
PF07648;    Kazal_2;    2.
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SMART
SM00274;    FOLN;    2.
SM00280;    KAZAL;    2.
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PROSITE
PS00282;    KAZAL_1;    FALSE_NEG.
PS51465;    KAZAL_2;    2.
PS51364;    TB;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
SIGNAL        1     23       Potential.
CHAIN        24    256       Follistatin-related protein 3.
                             /FTId=PRO_0000010117.
DOMAIN       34    105       TB.
DOMAIN       97    117       Follistatin-like 1.
DOMAIN      111    167       Kazal-like 1.
DOMAIN      168    191       Follistatin-like 2.
DOMAIN      187    243       Kazal-like 2.
CARBOHYD     71     71       N-linked (GlcNAc...) (Potential).
CARBOHYD    213    213       N-linked (GlcNAc...) (Potential).
DISULFID     36     59       By similarity.
DISULFID     46     90       By similarity.
DISULFID     60     93       By similarity.
DISULFID     97    108       By similarity.
DISULFID    102    117       By similarity.
DISULFID    119    151       By similarity.
DISULFID    123    144       By similarity.
DISULFID    133    165       By similarity.
DISULFID    193    227       By similarity.
DISULFID    198    220       By similarity.
DISULFID    209    241       By similarity.
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Nucleotide Sequence
Length: 771 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 256 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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