SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001901 
UniProt Accession
Theoretical PI
7.84  
Molecular Weight
34175 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Cyb5r3 
Gene Synonyms/Alias
Dia1 
Protein Name
NADH-cytochrome b5 reductase 3 NADH-cytochrome b5 reductase 3 membrane-bound form NADH-cytochrome b5 reductase 3 soluble form 
Protein Synonyms/Alias
B5RCytochrome b5 reductaseEC=1.6.2.2 Diaphorase-1;Contains:Contains: 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:7;121187821-121205398;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. E. Mironova and C. F. Millette (2008) Expression of the diaphanous-related formin proteins mDia1 and mDia2 in the rat testis. Dev Dyn 237(8): 2170-6. 

Abstract
Cytoskeletal alterations in both Sertoli cells and germ cells are important during many facets of mammalian spermatogenesis. Diaphanous-related formin proteins are known to control many aspects of actin-based cytoskeletal rearrangements, yet nothing is known regarding the expression of formins in the testis. Accordingly, here we present the first data describing mDia1 and mDia2 mRNA and protein expression in primary Sertoli cell isolates, established tissue culture cell lines often used as models for Sertoli cell analysis, and mixed populations of adult rat male germ cells. Furthermore, we have examined intact sections of rat testis. The results suggest strongly that mDia1 and mDia2 are indeed involved in the regulation of Sertoli cell and germ structure during mammalian spermatogenesis, and provide strong indications of the future directions for mechanistic studies. PMID: [18651670] 

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Figures for illustrating the function of this protein/gene
Ref: E. Mironova and C. F. Millette (2008) Expression of the diaphanous-related formin proteins mDia1 and mDia2 in the rat testis. Dev Dyn 237(8): 2170-6. PMID: [18651670]
Ref: E. Mironova and C. F. Millette (2008) Expression of the diaphanous-related formin proteins mDia1 and mDia2 in the rat testis. Dev Dyn 237(8): 2170-6. PMID: [18651670]
Ref: E. Mironova and C. F. Millette (2008) Expression of the diaphanous-related formin proteins mDia1 and mDia2 in the rat testis. Dev Dyn 237(8): 2170-6. PMID: [18651670]
Function
Desaturation and elongation of fatty acids, cholesterolbiosynthesis, drug metabolism, and, in erythrocyte, methemoglobinreduction. 
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Subcellular Location
Isoform 3: Cytoplasm. Note=Produces thesoluble form found in erythrocytes. 
Tissue Specificity
Isoform 1 and isoform 3 are ubiquitouslyexpressed. Isoform 2 is expressed only in erythroid tissues,reticulocytes and liver. 
Gene Ontology
GO IDGO termEvidence
GO:0005789 C:endoplasmic reticulum membrane IDA:HGNC.
GO:0005741 C:mitochondrial outer membrane IEA:UniProtKB-SubCell.
GO:0005625 C:soluble fraction TAS:RGD.
GO:0043531 F:ADP binding IDA:RGD.
GO:0016208 F:AMP binding IDA:RGD.
GO:0004128 F:cytochrome-b5 reductase activity TAS:RGD.
GO:0050660 F:flavin adenine dinucleotide binding IDA:RGD.
GO:0051287 F:NAD binding IDA:RGD.
GO:0006695 P:cholesterol biosynthetic process IEA:UniProtKB-KW.
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Interpro
IPR017927;    Fd_Rdtase_FAD-bd.
IPR001709;    Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834;    NADH-Cyt_B5_reductase.
IPR008333;    OxRdtase_FAD-bd_dom.
IPR001433;    OxRdtase_FAD/NAD-bd.
IPR017938;    Riboflavin_synthase-like_b-brl.
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Pfam
PF00970;    FAD_binding_6;    1.
PF00175;    NAD_binding_1;    1.
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SMART
PROSITE
PS51384;    FAD_FR;    1.
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PRINTS
PR00406;    CYTB5RDTASE.;   
PR00371;    FPNCR.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
INIT_MET      1      1       Removed.
CHAIN         2    301       NADH-cytochrome b5 reductase 3 membrane-
                             bound form.
                             /FTId=PRO_0000019401.
CHAIN        27    301       NADH-cytochrome b5 reductase 3 soluble
                             form.
                             /FTId=PRO_0000019403.
DOMAIN       40    152       FAD-binding FR-type.
NP_BIND     132    147       FAD.
NP_BIND     171    206       FAD.
MOD_RES      42     42       N6-acetyllysine (By similarity).
MOD_RES      43     43       Phosphotyrosine (By similarity).
MOD_RES     120    120       N6-acetyllysine (By similarity).
MOD_RES     130    130       Phosphotyrosine (By similarity).
LIPID         2      2       N-myristoyl glycine.
VAR_SEQ       1     23       Missing (in isoform 3).
                             /FTId=VSP_009660.
VAR_SEQ       1      7       MGAQLST -> MLGPLLWTASLPV (in isoform 2).
                             /FTId=VSP_009661.
CONFLICT    105    105       F -> L (in Ref. 2; AAA41008).
STRAND       43     52
STRAND       54     63
STRAND       78     85
STRAND       88     94
STRAND      104    111
STRAND      115    118
HELIX       126    132
STRAND      139    146
STRAND      148    153
STRAND      156    159
STRAND      168    171
STRAND      173    180
HELIX       181    183
HELIX       184    196
STRAND      203    212
HELIX       213    215
HELIX       219    228
TURN        230    232
STRAND      233    241
STRAND      247    252
HELIX       255    261
STRAND      269    275
HELIX       277    282
HELIX       285    291
HELIX       295    297
STRAND      298    300
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Nucleotide Sequence
Length: 1893 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 301 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Acsl1IntAct 
_IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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