SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001916 
UniProt Accession
Theoretical PI
6.86  
Molecular Weight
31492 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Casp3 
Gene Synonyms/Alias
Cpp32 
Protein Name
Caspase-3 Caspase-3 subunit p17 Caspase-3 subunit p12 
Protein Synonyms/Alias
CASP-3EC=3.4.22.56 Apopain; Cysteine protease CPP32;CPP-32 IRP; LICE; Protein Yama; SREBP cleavage activity 1;SCA-1Contains:Contains:Flags: Precursor 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:16;48944227-48962420;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. R. D. Moreno, C. Lizama, N. Urzua, S. P. Vergara and J. G. Reyes (2006) Caspase activation throughout the first wave of spermatogenesis in the rat. Cell Tissue Res 325(3): 533-40. 

Abstract
Early in postnatal life, the first wave of spermatogenesis is accompanied by an initial wave of germ cell apoptosis. This may reflect an adjustment in the number of germ cells that can be adequately maintained by Sertoli cells. Two major pathways (intrinsic and extrinsic) are involved in the process of caspase activation and apoptosis in mammalian cells. The extrinsic pathway is characterized by the oligomerization of death receptors such as FAS or tumor necrosis factor, followed by the activation of caspase-8 and caspase-3. The intrinsic pathway involves the activation of procaspase-9, which in turn activates caspase-3. Extensive information is available concerning apoptotic inducers and their possible mechanisms in the adult rat. However, no data exist regarding the molecular and cellular mechanisms governing physiological cell death during puberty in the male rat. We have studied caspase activation throughout the first wave of spermatogenesis in the rat under physiological conditions, by combining the TUNEL procedure with the localization of active caspases in germ cells. We observed TUNEL-positive germ cells in rats of 5-40 days of age, the highest number being found in 25-day-old rats. TUNEL-positive and caspase-3-positive germ cells appeared as long chains of interconnected germ cells in 25-day-old rats. Caspase activation was assayed by either immunohistochemistry with antibodies against active caspase-3, -8, and -9, or by determining enzymatic activity in seminiferous tubules extracts. Both techniques showed activation of caspase-3, -8, and -9 in 25-day-old rats and low enzymatic activity at other ages. Confocal scanning laser microscopy indicated that active caspase-3, -8, and -9 co-localized with TUNEL-positive cells. Thus, caspase-3, -8, and -9 are active in apoptotic germ cells during the first wave of rat spermatogenesis. The extrinsic pathway of apoptosis may therefore play an important role in germ cell apoptosis during puberty in the rat. PMID: [16598500] 

Back to Top
Figures for illustrating the function of this protein/gene
Function
Involved in the activation cascade of caspasesresponsible for apoptosis execution. At the onset of apoptosis itproteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a'216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatoryelement binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain.Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesionin sympathetic neurons through RET cleavage (By similarity). 
Back to Top
Subcellular Location
Cytoplasm. 
Tissue Specificity
Expressed in heart, brain, liver, and musclebut not in kidney or testis. 
Gene Ontology
GO IDGO termEvidence
GO:0005829 C:cytosol IDA:RGD.
GO:0005625 C:soluble fraction IDA:RGD.
GO:0004197 F:cysteine-type endopeptidase activity IEA:InterPro.
GO:0008234 F:cysteine-type peptidase activity IDA:RGD.
GO:0016005 F:phospholipase A2 activator activity IMP:RGD.
GO:0034349 P:glial cell apoptotic process IEP:RGD.
GO:0021766 P:hippocampus development IEP:RGD.
GO:0006917 P:induction of apoptosis TAS:RGD.
GO:0035556 P:intracellular signal transduction IMP:RGD.
GO:0007611 P:learning or memory IMP:RGD.
GO:0030182 P:neuron differentiation IEP:RGD.
GO:0043525 P:positive regulation of neuron apoptotic process IMP:RGD.
GO:0006508 P:proteolysis IDA:RGD.
GO:0043200 P:response to amino acid stimulus IEP:RGD.
GO:0046677 P:response to antibiotic IEP:RGD.
GO:0032025 P:response to cobalt ion IEP:RGD.
GO:0042493 P:response to drug IEP:RGD.
GO:0032355 P:response to estradiol stimulus IEP:RGD.
GO:0051384 P:response to glucocorticoid stimulus IEP:RGD.
GO:0009749 P:response to glucose stimulus IDA:RGD.
GO:0042542 P:response to hydrogen peroxide IDA:RGD.
GO:0001666 P:response to hypoxia IEP:RGD.
GO:0032496 P:response to lipopolysaccharide IEP:RGD.
GO:0035094 P:response to nicotine IEP:RGD.
GO:0010165 P:response to X-ray IEP:RGD.
GO:0042060 P:wound healing IEP:RGD.
Back to Top
Interpro
IPR015470;    Caspase_3.
IPR011600;    Pept_C14_cat.
IPR001309;    Pept_C14_ICE_p20.
IPR016129;    Pept_C14_ICE_p20_AS.
IPR002138;    Pept_C14_p10.
IPR002398;    Pept_C14_p45.
IPR015917;    Pept_C14_p45_core.
Back to Top
Pfam
PF00656;    Peptidase_C14;    1.
Back to Top
SMART
SM00115;    CASc;    1.
Back to Top
PROSITE
PS01122;    CASPASE_CYS;    1.
PS01121;    CASPASE_HIS;    1.
PS50207;    CASPASE_P10;    1.
PS50208;    CASPASE_P20;    1.
Back to Top
PRINTS
PR00376;    IL1BCENZYME.;   
Back to Top
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
PROPEP        1      9       By similarity.
                             /FTId=PRO_0000004589.
PROPEP       10     28       By similarity.
                             /FTId=PRO_0000004590.
CHAIN        29    175       Caspase-3 subunit p17.
                             /FTId=PRO_0000004591.
CHAIN       176    277       Caspase-3 subunit p12.
                             /FTId=PRO_0000004592.
ACT_SITE    121    121       By similarity.
ACT_SITE    163    163       By similarity.
MOD_RES       1      1       N-acetylmethionine (By similarity).
MOD_RES      26     26       Phosphoserine (By similarity).
MOD_RES      82     82       N6-acetyllysine (By similarity).
MOD_RES     163    163       S-nitrosocysteine; in inhibited form (By
                             similarity).
CONFLICT     25     29       KSMDS -> QVD (in Ref. 4; AAB02722).
CONFLICT    170    170       C -> S (in Ref. 5; AAC52261).
CONFLICT    178    178       T -> A (in Ref. 5; AAC52261).
CONFLICT    182    182       M -> V (in Ref. 5; AAC52261).
CONFLICT    187    187       I -> K (in Ref. 5; AAC52261).
CONFLICT    190    190       E -> G (in Ref. 2; AAB41792).
CONFLICT    199    199       T -> S (in Ref. 5; AAC52261).
CONFLICT    211    211       D -> G (in Ref. 5; AAC52261).
CONFLICT    236    236       L -> I (in Ref. 4; AAB02722).
CONFLICT    245    245       T -> M (in Ref. 2; AAB41792).
Back to Top
Nucleotide Sequence
Length: 1249 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 277 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Casp3MINT 
Casp3MINT 
TnfIntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
Comments