SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001975 
UniProt Accession
Theoretical PI
5.35  
Molecular Weight
60955 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Hspd1 
Gene Synonyms/Alias
Hsp60 
Protein Name
60 kDa heat shock protein, mitochondrial 
Protein Synonyms/Alias
60 kDa chaperonin; Chaperonin 60;CPN60 HSP-65; Heat shock protein 60;HSP-60Hsp60 Mitochondrial matrix protein P1;Flags: Precursor 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:9;53884193-53895043;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. A. Meinhardt, M. Parvinen, M. Bacher, G. Aumuller, H. Hakovirta, A. Yagi and J. Seitz (1995) Expression of mitochondrial heat shock protein 60 in distinct cell types and defined stages of rat seminiferous epithelium. Biol Reprod 52(4): 798-807. 

Abstract
Changes in the level of the gene transcript of heat shock protein (hsp)60, a mitochondrial chaperonin, during the cycle of rat seminiferous epithelium and its cellular localization were studied. The seminiferous epithelium showed a cell type-specific expression of hsp60. Immunostaining of adult rat testis revealed localization in Sertoli and Leydig cells. In germ cells, mitochondria of spermatogonia and early primary spermatocytes were immunoreactive for hsp60. Mitochondria of all other germ cell types were completely negative for hsp60. Stage-specific expression of hsp60 was determined from pooled segments of stage-specific microdissected tubules by a combination of Western blotting and polymerase chain reaction (PCR). High concentrations of hsp60 were found in stages I-V and IX-XIV, and low levels were detected in the other stages, i.e., VI-VIII. In stages with high hsp60 expression, spermatogonia divide mitotically, whereas in stages lacking mitosis, the hsp60 level was much weaker. In seminiferous epithelium, two different types of mitochondria are present. Therefore, immunoelectron microscopy was used to differentiate these two morphologically distinct types of mitochondria. The crista type of mitochondria (e.g., in Sertoli cells and spermatogonia) reacted with the antibody against hsp60, whereas hsp60 was negative in so-called "condensed"-type mitochondria found in midpachytene spermatocytes and more advanced germ cells. It could be shown for the first time that expression of the hsp60 gene is regulated during the cycle of the seminiferous epithelium. The results indicate that the gene product is primarily needed during the initial steps of spermatogenesis in which most of the cell divisions occur, while its expression during the differentiation of spermatids and sperm is obviously not necessary. The presence of hsp60 in stages with mitotic activity suggests a very active mitochondrial protein import and protein assembly machinery that generates further mitochondria for the dividing cells. PMID: [7780001] 

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Figures for illustrating the function of this protein/gene
Function
Implicated in mitochondrial protein import andmacromolecular assembly. May facilitate the correct folding ofimported proteins. May also prevent misfolding and promote therefolding and proper assembly of unfolded polypeptides generatedunder stress conditions in the mitochondrial matrix. 
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Subcellular Location
Mitochondrion matrix (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0009986 C:cell surface IDA:RGD.
GO:0005829 C:cytosol IDA:RGD.
GO:0005794 C:Golgi apparatus IDA:RGD.
GO:0045121 C:membrane raft IDA:RGD.
GO:0030061 C:mitochondrial crista IDA:RGD.
GO:0005759 C:mitochondrial matrix IEA:UniProtKB-SubCell.
GO:0005791 C:rough endoplasmic reticulum IDA:RGD.
GO:0042588 C:zymogen granule IDA:RGD.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0043559 F:insulin binding IPI:RGD.
GO:0006916 P:anti-apoptosis IMP:RGD.
GO:0051085 P:chaperone mediated protein folding requiring cofactor IDA:RGD.
GO:0002236 P:detection of misfolded protein IDA:RGD.
GO:0045768 P:positive regulation of anti-apoptosis IMP:RGD.
GO:0050729 P:positive regulation of inflammatory response IDA:RGD.
GO:0042026 P:protein refolding IEA:InterPro.
GO:0033198 P:response to ATP IEP:RGD.
GO:0042220 P:response to cocaine IEP:RGD.
GO:0042493 P:response to drug IEP:RGD.
GO:0043627 P:response to estrogen stimulus IEP:RGD.
GO:0009408 P:response to heat IEP:RGD.
GO:0042542 P:response to hydrogen peroxide IEP:RGD.
GO:0001666 P:response to hypoxia IEP:RGD.
GO:0032496 P:response to lipopolysaccharide IEP:RGD.
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Interpro
IPR018370;    Chaperonin_Cpn60_CS.
IPR001844;    Chaprnin_Cpn60.
IPR002423;    Cpn60/TCP-1.
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Pfam
PF00118;    Cpn60_TCP1;    1.
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SMART
PROSITE
PS00296;    CHAPERONINS_CPN60;    1.
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PRINTS
PR00298;    CHAPERONIN60.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
TRANSIT       1     26       Mitochondrion (By similarity).
CHAIN        27    573       60 kDa heat shock protein, mitochondrial.
                             /FTId=PRO_0000005029.
MOD_RES      31     31       N6-acetyllysine (By similarity).
MOD_RES      70     70       Phosphoserine (By similarity).
MOD_RES      82     82       N6-acetyllysine (By similarity).
MOD_RES     125    125       N6-acetyllysine (By similarity).
MOD_RES     130    130       N6-acetyllysine (By similarity).
MOD_RES     133    133       N6-malonyllysine (By similarity).
MOD_RES     202    202       N6-acetyllysine (By similarity).
MOD_RES     218    218       N6-acetyllysine (By similarity).
MOD_RES     223    223       Phosphotyrosine (By similarity).
MOD_RES     227    227       Phosphotyrosine (By similarity).
MOD_RES     269    269       N6-acetyllysine (By similarity).
MOD_RES     352    352       N6-acetyllysine (By similarity).
MOD_RES     359    359       N6-acetyllysine (By similarity).
MOD_RES     396    396       N6-acetyllysine (By similarity).
MOD_RES     469    469       N6-acetyllysine (By similarity).
MOD_RES     473    473       N6-acetyllysine (By similarity).
MOD_RES     523    523       N6-acetyllysine (By similarity).
CONFLICT    121    121       R -> P (in Ref. 2; AAC53362).
CONFLICT    537    537       S -> P (in Ref. 1; CAA38564).
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Nucleotide Sequence
Length: 2175 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 573 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
BaxMINT 
BaxMINT 
Slc2a4IntAct 
HnrnpkIntAct 
Mapk3MINT 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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