SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00001980 
UniProt Accession
Theoretical PI
5.39  
Molecular Weight
20820 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Bcl2l2 
Gene Synonyms/Alias
bcl-w 
Protein Name
 
Protein Synonyms/Alias
SubName: BCL-WSubName: Bcl-wSubName: Bcl2-like 2SubName: Protein Bcl2l2SubName: RCG23437, isoform CRA_b 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:15;32983165-32988017;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. W. Yan, M. Samson, B. Jegou and J. Toppari (2000) Bcl-w forms complexes with Bax and Bak, and elevated ratios of Bax/Bcl-w and Bak/Bcl-w correspond to spermatogonial and spermatocyte apoptosis in the testis. Mol Endocrinol 14(5): 682-99. 

Abstract
Bcl-w, a prosurvival member of the Bcl-2 family, is essential for spermatogenesis. However, the mechanisms by which Bcl-w participates in the regulation of apoptosis in the testis are largely unknown. To explore the potential role of Bcl-w in the regulation of apoptosis in the testis, the expression of Bcl-w mRNA and protein during testicular development and spermatogenesis, the dimerization with the proapoptosis members of the Bcl-2 family, and the responses to hormonal stimulation in vitro and apoptosis-inducing signals in vivo were investigated. Both Bcl-w mRNA and protein were detected in Sertoli cells, spermatogonia, and spermatocytes, as well as in Leydig cells. The steady-state levels of Bcl-w mRNA and protein were much higher in Sertoli cells than in spermatogonia and spermatocytes. In the adult rat testis, both Bcl-w mRNA and protein in Sertoli cells displayed a stage-specific expression pattern. Bcl-w could form complexes with Bax and Bak but not with Bad. Bax and Bak were immunohistochemically localized to the same cell types as Bcl-w, but with higher expression levels in spermatocytes and spermatogonia than in Sertoli cells. FSH could up-regulate Bcl-w mRNA levels in the seminiferous tubules cultured in vitro, whereas no effect was observed when testosterone was applied. Three animal models that display spermatogonial apoptosis induced by blockade of stem cell factor/c-kit interaction by a function-blocking anti-c-kit antibody, spermatocyte apoptosis induced by methoxyacetic acid, and apoptosis of spermatogonia, spermatocytes, and spermatids induced by testosterone withdrawal after ethylene dimethane sulfonate treatment were employed to check the changes of Bcl-w, Bax, and Bak protein levels during apoptosis of specific germ cells. In all three models, the ratios of Bax/Bcl-w and Bak/Bcl-w were significantly elevated. The present study suggests that Bcl-w is an important prosurvival factor of Sertoli cells, spermatogonia, and spermatocytes and participates in the regulation of apoptosis by binding proapoptotic factors Bax and Bak. The ratios of Bax/Bcl-w and Bak/Bcl-w may be decisive for the survival of Sertoli cells, spermatogonia, and spermatocytes. PMID: [10809232] 

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Figures for illustrating the function of this protein/gene
Ref: W. Yan, M. Samson, B. Jegou and J. Toppari (2000) Bcl-w forms complexes with Bax and Bak, and elevated ratios of Bax/Bcl-w and Bak/Bcl-w correspond to spermatogonial and spermatocyte apoptosis in the testis. Mol Endocrinol 14(5): 682-99. PMID: [10809232]
Ref: W. Yan, M. Samson, B. Jegou and J. Toppari (2000) Bcl-w forms complexes with Bax and Bak, and elevated ratios of Bax/Bcl-w and Bak/Bcl-w correspond to spermatogonial and spermatocyte apoptosis in the testis. Mol Endocrinol 14(5): 682-99. PMID: [10809232]
Ref: W. Yan, M. Samson, B. Jegou and J. Toppari (2000) Bcl-w forms complexes with Bax and Bak, and elevated ratios of Bax/Bcl-w and Bak/Bcl-w correspond to spermatogonial and spermatocyte apoptosis in the testis. Mol Endocrinol 14(5): 682-99. PMID: [10809232]
Ref: W. Yan, M. Samson, B. Jegou and J. Toppari (2000) Bcl-w forms complexes with Bax and Bak, and elevated ratios of Bax/Bcl-w and Bak/Bcl-w correspond to spermatogonial and spermatocyte apoptosis in the testis. Mol Endocrinol 14(5): 682-99. PMID: [10809232]
Ref: W. Yan, M. Samson, B. Jegou and J. Toppari (2000) Bcl-w forms complexes with Bax and Bak, and elevated ratios of Bax/Bcl-w and Bak/Bcl-w correspond to spermatogonial and spermatocyte apoptosis in the testis. Mol Endocrinol 14(5): 682-99. PMID: [10809232]
Ref: W. Yan, M. Samson, B. Jegou and J. Toppari (2000) Bcl-w forms complexes with Bax and Bak, and elevated ratios of Bax/Bcl-w and Bak/Bcl-w correspond to spermatogonial and spermatocyte apoptosis in the testis. Mol Endocrinol 14(5): 682-99. PMID: [10809232]
Function
 
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Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0042981 P:regulation of apoptotic process IEA:InterPro.
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Interpro
IPR013280;    Apop_reg_BclW.
IPR002475;    Bcl2-like.
IPR020717;    Bcl2_BH1_motif_CS.
IPR020726;    Bcl2_BH2_motif_CS.
IPR003093;    Bcl2_BH4.
IPR020731;    Bcl2_BH4_motif_CS.
IPR026298;    Blc2_fam.
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Pfam
PF00452;    Bcl-2;    1.
PF02180;    BH4;    1.
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SMART
SM00265;    BH4;    1.
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PROSITE
PS50062;    BCL2_FAMILY;    1.
PS01080;    BH1;    1.
PS01258;    BH2;    1.
PS01260;    BH4_1;    1.
PS50063;    BH4_2;    1.
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PRINTS
PR01865;    APOPREGBCLW.;   
PR01862;    BCL2FAMILY.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
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Nucleotide Sequence
Length: 582 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 193 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Bcl2l11IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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