SG ID

SG00001994 

UniProt Accession

CN37_RAT;P13233;Q4V796;Q64575;  

Theoretical PI

9.03  

Molecular Weight

47268 Da  

Genbank Nucleotide ID

M18630; L16532; BC098066;  

Genbank Protein ID

AAA40939.1; AAA64429.1; AAH98066.1; 

Gene Name

Cnp 

Gene Synonyms/Alias

Cnp1 

Protein Name

2',3'-cyclic-nucleotide 3'-phosphodiesterase 

Protein Synonyms/Alias

CNPCNPaseEC=3.1.4.37 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. W. Xia, D. D. Mruk and C. Y. Cheng (2007) C-type natriuretic peptide regulates blood-testis barrier dynamics in adult rat testes. Proc Natl Acad Sci U S A 104(10): 3841-6. 

Abstract
In adult rat testes, the blood-testis barrier (BTB) in the seminiferous epithelium must "open" (or "disassemble") to accommodate the migration of preleptotene spermatocytes from the basal to the adluminal compartment that occurs at stage VIII of the epithelial cycle. However, the molecule(s) and/or mechanism(s) that regulate this event are unknown. In this report, C-type natriuretic peptide (CNP) was shown to be a regulator of BTB dynamics. Although Sertoli and germ cells contributed to the pool of CNP in the seminiferous epithelium, its receptor, natriuretic peptide receptor B, resided almost exclusively in Sertoli cells. CNP also expressed stage-specifically and localized predominantly at the BTB in the seminiferous epithelium at stage VIII of the epithelial cycle. A synthetic CNP-22 peptide, when added to Sertoli cell cultures, was shown to perturb Sertoli cell tight junction in vitro, causing disappearance of BTB-associated proteins (JAM-A, occludin, N-cadherin, and beta-catenin) from the cell-cell interface. This inhibitory effect of CNP on the tight junction was confirmed by transient overexpression of CNP in these cells, which was mediated, at least in part, by accelerating the internalization of BTB integral membrane proteins. To validate these in vitro findings, CNP-22 was administered to testes at a dose of 0.35 or 3.5 mug per testis, which was shown to perturb the BTB integrity In vivo when the barrier function was assessed by monitoring the diffusion of a small molecular probe across the BTB. In summary, CNP secreted by Sertoli and germ cells into the BTB microenvironment regulates BTB dynamics during spermatogenesis. PMID: [17360440] 

2. D. H. Huang, S. W. Zhang, H. Zhao and L. Zhang (2011) The role of C-type natriuretic peptide in rat testes during spermatogenesis. Asian J Androl 13(2): 275-80. 

Abstract
C-type natriuretic peptide (CNP) is a 22-amino acid peptide and act as a local paracrine or autocrine regulator. There is growing evidence that CNP is involved in male reproductive processes. To investigate the role of CNP during spermatogenesis, we measured the mRNA expression of CNP and its specific membrane-bound natriuretic peptide receptor-B (NPR-B) using real-time RT-PCR in the testes of normal rats on different postnatal days. After that spermatogenesis dysfunction model induced by ornidazole was established with the aim to study the correlation of CNP with spermatogenic dysfunction. Then, Sertoli cells from 18- to 22-day-old healthy male rats were cultured in the presence of different CNP concentrations (1x10(-6), 1x10(-7) and 1x10(-8) mol l(-1)), and the mRNA expression levels of androgen-binding protein, inhibin B and transferrin were examined at 0 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h and 48 h. During the postnatal development of rat testes, the highest mRNA expression levels of CNP and NPR-B were found at postnatal D(0), and the levels then declined gradually, with a second CNP peak at postnatal D(35). In the ornidazole-induced infertile rat testes, CNP gene expression was lower than in the uninduced rats (P<0.05), while NPR-B gene expression was greater (P<0.05). In cultured Sertoli cells, supplementation with CNP stimulated the gene expression of androgen-binding protein/inhibin B/transferrin, particularly at 12 h, and 1x10(-7) mol l(-1) CNP had the highest upregulation effect. The gene expression levels of CNP/NPR-B in rat testes at different postnatal stages and in infertile rat testes indicated that CNP may participate in the physiology and/or pathology related to spermatogenesis. Moreover, CNP regulated endocrine function in Sertoli cells. Taken together, these results showed that CNP is closely tied to spermatogenesis. PMID: [21170077] 

Figures for illustrating the function of this protein/gene

Function

May participate in RNA metabolism in the myelinatingcell, CNP is the third most abundant protein in central nervoussystem myelin (By similarity). 

Subcellular Location

Membrane; Lipid-anchor. Melanosome (Bysimilarity). Note=Firmly bound to membrane structures of brainwhite matter. 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005615	C:extracellular space	IEA:Compara.
GO:0042470	C:melanosome	IEA:UniProtKB-SubCell.
GO:0005624	C:membrane fraction	IEA:Compara.
GO:0005874	C:microtubule	IEA:Compara.
GO:0005902	C:microvillus	IDA:RGD.
GO:0005743	C:mitochondrial inner membrane	IDA:RGD.
GO:0005741	C:mitochondrial outer membrane	IDA:RGD.
GO:0035748	C:myelin sheath abaxonal region	IDA:RGD.
GO:0035749	C:myelin sheath adaxonal region	IDA:RGD.
GO:0048471	C:perinuclear region of cytoplasm	IDA:RGD.
GO:0005886	C:plasma membrane	IDA:RGD.
GO:0031143	C:pseudopodium	IDA:RGD.
GO:0004113	F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity	IDA:RGD.
GO:0030551	F:cyclic nucleotide binding	IDA:RGD.
GO:0003723	F:RNA binding	IEA:UniProtKB-KW.
GO:0008344	P:adult locomotory behavior	IEA:Compara.
GO:0007568	P:aging	IEP:RGD.
GO:0007409	P:axonogenesis	IEA:Compara.
GO:0009214	P:cyclic nucleotide catabolic process	IEA:InterPro.
GO:0030900	P:forebrain development	IEP:RGD.
GO:0000226	P:microtubule cytoskeleton organization	IMP:RGD.
GO:0046902	P:regulation of mitochondrial membrane permeability	IMP:RGD.
GO:0032496	P:response to lipopolysaccharide	IEP:RGD.
GO:0009636	P:response to toxin	IEA:Compara.
GO:0016070	P:RNA metabolic process	IEA:InterPro.

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Interpro

IPR008431;    CNPase.
IPR009097;    RNA_ligase/cNuc_Pdiesterase.
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Pfam

PF05881;    CNPase;    1.
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SMART

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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 2019 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 420 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
RD23B_RAT	Rad23b	IntAct

Other Protein-Protein interaction resources

String database  

UniProt	Gene Symbol	Ref Databases
5NTD_RAT	Nt5e	String
ANFC_RAT	Nppc	String
ASCL1_RAT	Ascl1	String
CD123_RAT	Cdc123	String
5NTD_RAT	Nt5e	String
ANFC_RAT	Nppc	String
ASCL1_RAT	Ascl1	String
CD123_RAT	Cdc123	String
CNTF_RAT	Cntf	String
CSRP1_RAT	Csrp1	String
DLG4_RAT	Dlg4	String
EAF2_RAT	Eaf2	String
ERMIN_RAT	Ermn	String
GFAP_RAT	Gfap	String
GPDA_RAT	Gpd1	String
IBP6_RAT	Igfbp6	String
MAG_RAT	Mag	String
MOG_RAT	Mog	String
MYPR_RAT	Plp1	String
OLIG1_RAT	Olig1	String
OREX_RAT	Hcrt	String
PLLP_RAT	Pllp	String
PRIO_RAT	Prnp	String
PTPM1_RAT	Ptpmt1	String
PTRF_RAT	Ptrf	String
RD23B_RAT	Rad23b	String
RNS1B_RAT	Rnase1	String
SOX10_RAT	Sox10	String
TNR16_RAT	Ngfr	String
CNTF_RAT	Cntf	String
CSRP1_RAT	Csrp1	String
DLG4_RAT	Dlg4	String
EAF2_RAT	Eaf2	String
ERMIN_RAT	Ermn	String
GFAP_RAT	Gfap	String
GPDA_RAT	Gpd1	String
IBP6_RAT	Igfbp6	String
MAG_RAT	Mag	String
MOG_RAT	Mog	String
MYPR_RAT	Plp1	String
OLIG1_RAT	Olig1	String
OREX_RAT	Hcrt	String
PLLP_RAT	Pllp	String
PRIO_RAT	Prnp	String
PTPM1_RAT	Ptpmt1	String
PTRF_RAT	Ptrf	String
RNS1B_RAT	Rnase1	String
SOX10_RAT	Sox10	String
TNR16_RAT	Ngfr	String

View Microarray data

Temporarily unavailable 

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