SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00002012 
UniProt Accession
Theoretical PI
8.69  
Molecular Weight
38327 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Polb 
Gene Synonyms/Alias
 
Protein Name
DNA polymerase beta 
Protein Synonyms/Alias
EC=2.7.7.7EC=4.2.99.- 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:16;73864707-73890113;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. A. W. Plug, C. A. Clairmont, E. Sapi, T. Ashley and J. B. Sweasy (1997) Evidence for a role for DNA polymerase beta in mammalian meiosis. Proc Natl Acad Sci U S A 94(4): 1327-31. 

Abstract
DNA polymerase beta (pol beta) is an enzyme possessing both polymerase and deoxyribose phophatase activities. Although pol beta is not believed to participate in the replication of genomic DNA, several studies have indicated a role for pol beta in DNA repair. The high level of expression of pol beta in mouse and rat testes raises the possibility that pol beta participates in mammalian meiosis. Using antibody localization, we detect foci that stain with pol beta antisera at discrete sites along homologous chromosomes as they synapse and progress through prophase of meiosis I. These data suggest that pol beta participates in meiotic events associated with synapsis and recombination. PMID: [9037052] 

Back to Top
Figures for illustrating the function of this protein/gene
Function
Repair polymerase that plays a key role in base-excisionrepair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activitythat removes the 5' sugar phosphate and also acts as a DNApolymerase that adds one nucleotide to the 3' end of the arisingsingle-nucleotide gap. Conducts 'gap-filling' DNA synthesis in astepwise distributive fashion rather than in a processive fashionas for other DNA polymerases. 
Back to Top
Subcellular Location
Nucleus. Cytoplasm (By similarity).Note=Cytoplasmic in normal conditions. Translocates to the nucleusfollowing DNA damage (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm ISS:UniProtKB.
GO:0005634 C:nucleus IDA:RGD.
GO:0005876 C:spindle microtubule IEA:Compara.
GO:0003684 F:damaged DNA binding IDA:RGD.
GO:0003887 F:DNA-directed DNA polymerase activity ISS:UniProtKB.
GO:0016829 F:lyase activity ISS:UniProtKB.
GO:0046872 F:metal ion binding IDA:UniProtKB.
GO:0006916 P:anti-apoptosis IEA:Compara.
GO:0006284 P:base-excision repair ISS:UniProtKB.
GO:0008219 P:cell death IEA:Compara.
GO:0006260 P:DNA replication IEA:UniProtKB-KW.
GO:0006290 P:pyrimidine dimer repair IDA:RGD.
GO:0045471 P:response to ethanol IEP:RGD.
Back to Top
Interpro
IPR002054;    DNA-dir_DNA_pol_X.
IPR010996;    DNA-dir_DNA_pol_X_beta-like_N.
IPR019843;    DNA_pol-X_BS.
IPR018944;    DNA_pol_lambd_fingers_domain.
IPR022312;    DNA_pol_X.
IPR002008;    DNA_pol_X_beta-like.
IPR003583;    Hlx-hairpin-Hlx_DNA-bd_motif.
Back to Top
Pfam
PF10391;    DNA_pol_lambd_f;    1.
Back to Top
SMART
SM00278;    HhH1;    2.
SM00483;    POLXc;    1.
Back to Top
PROSITE
PS00522;    DNA_POLYMERASE_X;    1.
Back to Top
PRINTS
PR00869;    DNAPOLX.;   
PR00870;    DNAPOLXBETA.;   
Back to Top
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
INIT_MET      1      1       Removed.
CHAIN         2    335       DNA polymerase beta.
                             /FTId=PRO_0000218780.
REGION      183    192       DNA binding.
ACT_SITE     72     72       Schiff-base intermediate with DNA (By
                             similarity).
METAL       101    101       Sodium; via carbonyl oxygen.
METAL       103    103       Sodium; via carbonyl oxygen.
METAL       106    106       Sodium; via carbonyl oxygen.
METAL       190    190       Magnesium 1.
METAL       190    190       Magnesium 2.
METAL       192    192       Magnesium 1.
METAL       192    192       Magnesium 2.
METAL       256    256       Magnesium 2.
MOD_RES      83     83       Omega-N-methylarginine; by PRMT6 (By
                             similarity).
MOD_RES     152    152       Omega-N-methylarginine; by PRMT6 (By
                             similarity).
CROSSLNK     41     41       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     61     61       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     81     81       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
MUTAGEN     190    190       D->E,S: Loss of activity.
MUTAGEN     191    191       M->I: No loss of activity.
MUTAGEN     191    191       M->T: 50% loss of activity.
MUTAGEN     192    192       D->E,S: Loss of activity.
MUTAGEN     246    246       D->V: Misincorporates T nucleotide
                             opposite G/C template.
CONFLICT    228    228       L -> R (in Ref. 1; AAA41901, 2; AAB00389
                             and 4; AAA41900).
HELIX        13     28
STRAND       29     31
HELIX        34     48
HELIX        56     60
HELIX        67     78
STRAND       79     81
HELIX        83     90
HELIX        92    100
STRAND      103    105
HELIX       108    115
TURN        116    118
HELIX       122    126
HELIX       129    131
HELIX       134    141
TURN        142    144
HELIX       145    147
HELIX       152    169
STRAND      174    177
HELIX       179    182
STRAND      186    196
TURN        202    204
HELIX       208    220
STRAND      224    230
STRAND      232    239
STRAND      245    247
STRAND      253    259
HELIX       262    264
HELIX       265    273
HELIX       276    288
STRAND      291    293
STRAND      298    301
STRAND      303    305
HELIX       316    322
HELIX       330    332
Back to Top
Nucleotide Sequence
Length: 1008 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 335 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
CdiptString 
CdiptString 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
Comments