SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00002013 
UniProt Accession
Theoretical PI
5.36  
Molecular Weight
117788 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Uba1 
Gene Synonyms/Alias
Ube1 
Protein Name
Ubiquitin-like modifier-activating enzyme 1 
Protein Synonyms/Alias
Ubiquitin-activating enzyme E1; 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:X;12926071-12944522;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. Y. Du, M. L. Liu and M. C. Jia (2008) Identification and characterization of a spermatogenesis-related gene Ube1 in rat testis. Sheng Li Xue Bao 60(3): 382-90. 

Abstract
A gene that could be potentially involved in spermatogenesis was identified and characterized by using suppression subtractive hybridization (SSH) and rapid amplification of cDNA ends (RACE) with total RNA from type A spermatogonia and pachytene spermatocytes of rat. This gene consists of 3 433 base pairs (bp) with a complete open reading frame (ORF) of 3 171 bp and encodes a putative protein containing 1057 amino acids. The nucleotide sequence displays a 93% identity to mouse ubiquitin-activating enzyme E1, Chr Y 1 (Ube1y1) and an 82% identity to human ubiquitin-activating enzyme E1 (UBE1). The putative protein of this gene contains an ubiquitin-activating enzyme signature 1 and an ubiquitin-activating enzyme active site, which are also existed in mouse ubiquitin-activating enzyme E1, human ubiquitin-activating enzyme E1 et al. So we named this gene as Rattus norvegicus ubiquitin-activating enzyme E1 (Ube1). The sequence of Ube1 was submitted to GenBank and the accession number is EF690356. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that Ube1 was specifically expressed in testis, while its expression was not detected in heart, brain, spleen, lung, liver, muscle, kidney and ovary. Comparison of the expression of Ube1 in different developmental stages of testis and Sertoli cells (real-time PCR) indicated that Ube1 was expressed more highly in spermatogonia than in spermatocytes, spermatids and Sertoli cells. In conclusion, Ube1 is a gene encoding rat ubiquitin-activating enzyme E1 and specifically expressed in testis, which might play a key role in ubiquitin system and influence spermatogenesis. PMID: [18560730] 

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Figures for illustrating the function of this protein/gene
Function
Activates ubiquitin by first adenylating its C-terminalglycine residue with ATP, and thereafter linking this residue tothe side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP (By similarity). 
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Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0016874 F:ligase activity IEA:UniProtKB-KW.
GO:0008641 F:small protein activating enzyme activity IEA:InterPro.
GO:0016567 P:protein ubiquitination IEA:UniProtKB-UniPathway.
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Interpro
IPR009036;    Molybdenum_cofac_synth_MoeB.
IPR016040;    NAD(P)-bd_dom.
IPR000594;    ThiF_NAD_FAD-bd.
IPR018965;    Ub-activating_enz_e1_C.
IPR023280;    Ub-like_act_enz_cat_cys_dom.
IPR000127;    UBact_repeat.
IPR019572;    Ubiquitin-activating_enzyme.
IPR018075;    UBQ-activ_enz_E1.
IPR018074;    UBQ-activ_enz_E1_AS.
IPR000011;    UBQ/SUMO-activ_enz_E1-like.
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Pfam
PF00899;    ThiF;    2.
PF09358;    UBA_e1_C;    1.
PF10585;    UBA_e1_thiolCys;    1.
PF02134;    UBACT;    2.
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SMART
SM00985;    UBA_e1_C;    1.
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PROSITE
PS00536;    UBIQUITIN_ACTIVAT_1;    1.
PS00865;    UBIQUITIN_ACTIVAT_2;    1.
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PRINTS
PR01849;    UBIQUITINACT.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1058       Ubiquitin-like modifier-activating enzyme
                             1.
                             /FTId=PRO_0000365096.
REPEAT       63    199       1-1.
REPEAT      459    611       1-2.
NP_BIND     478    507       ATP (By similarity).
REGION       63    611       2 approximate repeats.
ACT_SITE    632    632       Glycyl thioester intermediate (By
                             similarity).
MOD_RES       4      4       Phosphoserine (By similarity).
MOD_RES      13     13       Phosphoserine (By similarity).
MOD_RES      21     21       Phosphoserine (By similarity).
MOD_RES      24     24       Phosphoserine (By similarity).
MOD_RES      31     31       Phosphoserine (By similarity).
MOD_RES      46     46       Phosphoserine (By similarity).
MOD_RES      55     55       Phosphotyrosine (By similarity).
MOD_RES     671    671       N6-acetyllysine (By similarity).
MOD_RES     800    800       Phosphothreonine (By similarity).
MOD_RES     810    810       Phosphoserine (By similarity).
MOD_RES     816    816       Phosphoserine (By similarity).
MOD_RES     820    820       Phosphoserine (By similarity).
MOD_RES     835    835       Phosphoserine (By similarity).
MOD_RES     980    980       N6-acetyllysine (By similarity).
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Nucleotide Sequence
Length: bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1058 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
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