SG ID

SG00002032 

UniProt Accession

G3V8P3_RAT;G3V8P3;  

Theoretical PI

5.26  

Molecular Weight

317122 Da  

Genbank Nucleotide ID

CH473952;  

Genbank Protein ID

EDL81937.1; 

Gene Name

Celsr2 

Gene Synonyms/Alias

ORFNames=rCG_28504 

Protein Name

 

Protein Synonyms/Alias

SubName: Cadherin EGF LAG seven-pass G-type receptor 2SubName: RCG28504 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. S. A. Beall, K. Boekelheide and K. J. Johnson (2005) Hybrid GPCR/cadherin (Celsr) proteins in rat testis are expressed with cell type specificity and exhibit differential Sertoli cell-germ cell adhesion activity. J Androl 26(4): 529-38. 

Abstract
Spermatogenesis requires Sertoli cell-germ cell adhesion for germ cell survival and maturation. Cadherins are a diverse superfamily of adhesion proteins; structurally unique members of this superfamily (celsr cadherins) are hybrid molecules containing extracellular cadherin repeats connected to a G protein-coupled receptor transmembrane motif. Here we demonstrate postnatal testicular mRNA expression of the 3 celsr paralogs (celsr1, celsr2, and celsr3), protein localization of celsr2 and celsr3, and functional analysis of celsr2 adhesion activity in primary Sertoli cell-germ cell co-cultures. Evaluation of celsr mRNA levels during a postnatal time course indicated that celsr1 and celsr2 were Sertoli cell and/or early-stage germ cell products, whereas celsr3 was expressed in later-stage germ cells. Cell type-specific expression was verified using the Sertoli cell line 93RS2, where celsr1 and celsr2 mRNA, but not celsr3, were detected. Immunostaining of testicular cryosections resulted in celsr2 protein localization to a spokelike pattern in the basal seminiferous epithelium and punctate figures in the apical epithelium, consistent with both Sertoli cell and germ cell expression. Celsr3 localized to punctate structures in the adluminal epithelium from postnatal day 40, consistent with elongate spermatid expression. The subcellular localization of celsr2 was examined further to define its localization in Sertoli cells and germ cells. Celsr2 localized to the Golgi complex in Sertoli cells and germ cells. In addition, germ cell celsr2 localized to a rab7-positive structure, which may be an endocytic compartment. Neither celsr2 nor celsr3 immunostaining was present at classic cadherin-based adhesion junctions. Nonetheless, the addition of a recombinant celsr2 protein fragment consisting of extracellular cadherin domains 4 through 8 to Sertoli cell-germ cell co-cultures resulted in germ cell detachment from Sertoli cells. Collectively, these data indicate that celsr cadherins have a cell type-specific expression pattern, and celsr2 may mediate Sertoli cell-germ cell adhesion outside of classic cadherin-based adhesion junctions. PMID: [15955893] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

Cell membrane; Multi-pass membrane protein(By similarity). 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0016021	C:integral to membrane	IEA:UniProtKB-KW.
GO:0005886	C:plasma membrane	IEA:UniProtKB-SubCell.
GO:0005509	F:calcium ion binding	IEA:InterPro.
GO:0004930	F:G-protein coupled receptor activity	IEA:UniProtKB-KW.
GO:0007156	P:homophilic cell adhesion	IEA:InterPro.
GO:0007218	P:neuropeptide signaling pathway	IEA:InterPro.

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Interpro

IPR002126;    Cadherin.
IPR015919;    Cadherin-like.
IPR020894;    Cadherin_CS.
IPR008985;    ConA-like_lec_gl.
IPR013320;    ConA-like_subgrp.
IPR022624;    DUF3497.
IPR000742;    EG-like_dom.
IPR006210;    EGF-like.
IPR001881;    EGF-like_Ca-bd.
IPR013032;    EGF-like_CS.
IPR006209;    EGF-like_dom.
IPR000152;    EGF-type_Asp/Asn_hydroxyl_site.
IPR002049;    EGF_laminin.
IPR017981;    GPCR_2-like.
IPR001879;    GPCR_2_extracellular_dom.
IPR000832;    GPCR_2_secretin-like.
IPR000203;    GPS_dom.
IPR001791;    Laminin_G.
IPR001368;    TNFR/NGFR_Cys_rich_reg.
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Pfam

PF00002;    7tm_2;    1.
PF00028;    Cadherin;    8.
PF12003;    DUF3497;    1.
PF00008;    EGF;    2.
PF01825;    GPS;    1.
PF00053;    Laminin_EGF;    1.
PF02210;    Laminin_G_2;    2.
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SMART

SM00112;    CA;    9.
SM00181;    EGF;    5.
SM00179;    EGF_CA;    1.
SM00180;    EGF_Lam;    1.
SM00303;    GPS;    1.
SM00008;    HormR;    1.
SM00282;    LamG;    2.
SM00208;    TNFR;    1.
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PROSITE

PS00010;    ASX_HYDROXYL;    2.
PS00232;    CADHERIN_1;    7.
PS50268;    CADHERIN_2;    9.
PS00022;    EGF_1;    6.
PS01186;    EGF_2;    4.
PS50026;    EGF_3;    6.
PS01248;    EGF_LAM_1;    1.
PS50027;    EGF_LAM_2;    2.
PS50227;    G_PROTEIN_RECEP_F2_3;    1.
PS50261;    G_PROTEIN_RECEP_F2_4;    1.
PS50221;    GPS;    1.
PS50025;    LAM_G_DOMAIN;    2.
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PRINTS

PR00205;    CADHERIN.;   
PR00249;    GPCRSECRETIN.;   
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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 2919 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

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