SG ID

SG00002061 

UniProt Accession

G3V9J6_RAT;G3V9J6;  

Theoretical PI

8.7  

Molecular Weight

141253 Da  

Genbank Nucleotide ID

CH474013;  

Genbank Protein ID

EDL84316.1; 

Gene Name

Abcb1b 

Gene Synonyms/Alias

ORFNames=rCG_41101 

Protein Name

 

Protein Synonyms/Alias

SubName: Protein Abcb1bSubName: RCG41101 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. L. Su, C. Y. Cheng and D. D. Mruk (2009) Drug transporter, P-glycoprotein (MDR1), is an integrated component of the mammalian blood-testis barrier. Int J Biochem Cell Biol 41(12): 2578-87. 

Abstract
Throughout spermatogenesis, leptotene spermatocytes traverse the blood-testis barrier (BTB) to enter the adluminal compartment of the seminiferous epithelium for continued development. At the same time, the integrity of the BTB, which is constituted by co-existing tight junctions (TJ), basal ectoplasmic specializations (basal ES) and desmosome-like junctions, must be maintained since a breach in barrier function can result in spermatogenic arrest and even infertility. There is evidence to suggest that drug transporters may function at the BTB, but little is known about how they contribute to spermatogenesis. In this study, we investigate the role of P-glycoprotein (P-gp), a drug efflux pump, in BTB dynamics. A survey by RT-PCR revealed several transporter genes to be expressed by the testis, including Mdr1 (gene symbol for P-gp), Mrp1, Abcc5 and Slc15a1. It was also demonstrated that P-gp localizes to the BTB in all stages of the seminiferous epithelial cycle in the adult rat testis, as well as to the Sertoli cell-elongated spermatid interface in stages VII and VIII. We continued our study by examining the levels of several transporters in the testis following oral administration of Adjudin, a compound known to affect Sertoli-germ cell adhesion. In this experiment, the steady-state levels of P-gp, MRP1, ABCG1 and SLC15A1 were all found to increase by several-fold within hours of Adjudin treatment during junction restructuring. More importantly, an increase in P-gp association with TJ proteins (e.g., occludin, claudin-11 and JAM-A) was noted when testis lysates from Adjudin-treated rats were used for co-immunoprecipitation experiments, suggesting that P-gp may enhance BTB function during Sertoli-germ cell junction restructuring. PMID: [19720156] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0016021	C:integral to membrane	IEA:InterPro.
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0042626	F:ATPase activity, coupled to transmembrane movement of substances	IEA:InterPro.
GO:0006200	P:ATP catabolic process	IEA:GOC.

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Interpro

IPR003593;    AAA+_ATPase.
IPR003439;    ABC_transporter-like.
IPR017871;    ABC_transporter_CS.
IPR017940;    ABC_transporter_type1.
IPR001140;    ABC_transptr_TM_dom.
IPR011527;    ABC_transptrTM_dom_typ1.
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Pfam

PF00664;    ABC_membrane;    2.
PF00005;    ABC_tran;    2.
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SMART

SM00382;    AAA;    2.
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PROSITE

PS50929;    ABC_TM1F;    2.
PS00211;    ABC_TRANSPORTER_1;    2.
PS50893;    ABC_TRANSPORTER_2;    2.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 1275 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

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