SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00002071 
UniProt Accession
Theoretical PI
8.77  
Molecular Weight
40963 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Ghsr 
Gene Synonyms/Alias
 
Protein Name
Growth hormone secretagogue receptor type 1 
Protein Synonyms/Alias
GHS-R GH-releasing peptide receptor;GHRP Ghrelin receptor; 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:2;113269623-113272999;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. A. Lukaszyk, L. Rafinska, P. Sawinski, A. Kasprzak, K. Olejniczak, M. Rucinski, M. Ruchala and J. Sowinski (2009) Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium. Folia Histochem Cytobiol 47(3): 415-23. 

Abstract
The results of presented study demonstrate expression of ghrelin, its functional receptor GHSR-1a and their genes in spermatogenic cells of rat testis suggesting their functioning within seminiferous epithelium. The immunohistochemical and hybrydocytochemical expression, of proteins and transcripts, was estimated taking into account the cycle of seminiferous epithelium and phases of spermatogenesis. Both transcripts and ghrelin was found to show nuclear expression and scarcely cytoplasmic. Expression of genes for ghrelin and GHSR-1a was shown in early spermatocytes and round spermatids representing transcriptional phases of meiosis and spermiogenesis. Ghrelin was evidenced to show nuclear expression in two stage-specific windows, in late spermatogonia, in spermatocytes up to early pachytenes, and again in spermatids of acrosome and early maturation phase of spermiogenesis. In late pachytenes, secondary spermatocytes, round spermatids, maturing spermatids and spermatozoa the reaction is lacking. With two types of antibodies against the GHSR-1a used the two different patterns of immunostaining was evidenced suggesting two isoforms of GHSR-1a. The first evidenced GHSR-1a in cytoplasm of spermatocytes, cell membrane and acrosomes of spermatids, Sertoli cell processes and heads of spermatozoa. With second type of antibodies the immunostaining marks all steps of evolution of acrosome in spermatids. It is believed that site of ghrelin expression in seminiferous epithelium may indicate its role in local regulations, not excepting the intracellular signalling. Immunostaining pattern for GHSR-1a seems to suggest both its participation in the cross-talk among the cells and also process of furnishing gametes with GHSR-1a for its response to ghrelin in seminal plasma or female reproductive tract. PMID: [20164026] 

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Figures for illustrating the function of this protein/gene
Ref: A. Lukaszyk, L. Rafinska, P. Sawinski, A. Kasprzak, K. Olejniczak, M. Rucinski, M. Ruchala and J. Sowinski (2009) Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium. Folia Histochem Cytobiol 47(3): 415-23. PMID: [20164026]
Function
Receptor for ghrelin, coupled to G-alpha-11 proteins.Stimulates growth hormone secretion. Binds also other growthhormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6)as well as non-peptide, low molecular weight secretagogues (e.g.L-692,429, MK-0677, adenosine) (By similarity). 
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Subcellular Location
Cell membrane; Multi-pass membrane protein. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0009986 C:cell surface ISS:HGNC.
GO:0016021 C:integral to membrane TAS:RGD.
GO:0045121 C:membrane raft ISS:UniProtKB.
GO:0043005 C:neuron projection ISS:UniProtKB.
GO:0005886 C:plasma membrane IEA:UniProtKB-SubCell.
GO:0001616 F:growth hormone secretagogue receptor activity ISS:HGNC.
GO:0016520 F:growth hormone-releasing hormone receptor activity ISS:HGNC.
GO:0017046 F:peptide hormone binding IMP:RGD.
GO:0008154 P:actin polymerization or depolymerization ISS:UniProtKB.
GO:0008343 P:adult feeding behavior ISS:HGNC.
GO:0032869 P:cellular response to insulin stimulus IEA:Compara.
GO:0046697 P:decidualization ISS:UniProtKB.
GO:0030252 P:growth hormone secretion IEA:Compara.
GO:0009755 P:hormone-mediated signaling pathway ISS:HGNC.
GO:0050728 P:negative regulation of inflammatory response ISS:UniProtKB.
GO:0046676 P:negative regulation of insulin secretion IDA:MGI.
GO:0032691 P:negative regulation of interleukin-1 beta production ISS:UniProtKB.
GO:0045409 P:negative regulation of interleukin-6 biosynthetic process ISS:UniProtKB.
GO:0042536 P:negative regulation of tumor necrosis factor biosynthetic process ISS:UniProtKB.
GO:0032100 P:positive regulation of appetite ISS:HGNC.
GO:0045923 P:positive regulation of fatty acid metabolic process IMP:RGD.
GO:0043568 P:positive regulation of insulin-like growth factor receptor signaling pathway IEA:Compara.
GO:0040018 P:positive regulation of multicellular organism growth ISS:HGNC.
GO:0043134 P:regulation of hindgut contraction IMP:RGD.
GO:0051963 P:regulation of synapse assembly IEA:Compara.
GO:0032094 P:response to food IEA:Compara.
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Interpro
IPR000276;    7TM_GPCR_Rhodpsn.
IPR003905;    GHS1_rcpt.
IPR017452;    GPCR_Rhodpsn_supfam.
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Pfam
PF00001;    7tm_1;    1.
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SMART
PROSITE
PS00237;    G_PROTEIN_RECEP_F1_1;    1.
PS50262;    G_PROTEIN_RECEP_F1_2;    1.
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PRINTS
PR01417;    GHSRECEPTOR.;   
PR00237;    GPCRRHODOPSN.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    364       Growth hormone secretagogue receptor type
                             1.
                             /FTId=PRO_0000069482.
TOPO_DOM      1     40       Extracellular (Potential).
TRANSMEM     41     66       Helical; Name=1; (Potential).
TOPO_DOM     67     72       Cytoplasmic (Potential).
TRANSMEM     73     96       Helical; Name=2; (Potential).
TOPO_DOM     97    117       Extracellular (Potential).
TRANSMEM    118    139       Helical; Name=3; (Potential).
TOPO_DOM    140    162       Cytoplasmic (Potential).
TRANSMEM    163    183       Helical; Name=4; (Potential).
TOPO_DOM    184    211       Extracellular (Potential).
TRANSMEM    212    235       Helical; Name=5; (Potential).
TOPO_DOM    236    263       Cytoplasmic (Potential).
TRANSMEM    264    285       Helical; Name=6; (Potential).
TOPO_DOM    286    302       Extracellular (Potential).
TRANSMEM    303    326       Helical; Name=7; (Potential).
TOPO_DOM    327    364       Cytoplasmic (Potential).
CARBOHYD     13     13       N-linked (GlcNAc...) (Potential).
CARBOHYD     26     26       N-linked (GlcNAc...) (Potential).
CARBOHYD    187    187       N-linked (GlcNAc...) (Potential).
DISULFID    115    197       By similarity.
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Nucleotide Sequence
Length: 3129 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 364 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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