1. M. P. de Miguel, M. de Boer-Brouwer, D. G. de Rooij, R. Paniagua and F. M. van Dissel-Emiliani (1997) Ontogeny and localization of an oncostatin M-like protein in the rat testis. Cell Growth Differ 8(5): 611-8.
Abstract Oncostatin M (OSM), a member of the interleukin 6 family of cytokines, was found to be highly expressed in the late fetal and early neonatal rat testis, as well as in the maturing and adult testis. Two different forms of OSM were observed, one of M(r) 22,000 and the other of M(r) 36,000. In the prepubertal rat testis [19 days post coitum, 8 days post partum (dpp), and 15 dpp], the form with the higher molecular weight prevailed, whereas in the maturing testis (30 dpp, 45 dpp, and 12 weeks post partum), a shift toward the lower molecular weight form was observed, as well as a decrease in its relative amount. By immunohistochemistry on testicular sections, OSM-specific immunostaining was observed in the interstitial tissue at every age studied. In contrast, OSM immunoreaction was localized in the Sertoli cells exclusively around the start of spermatogenesis, being strongest at 3 dpp. In vitro studies revealed that neonatal Sertoli cells produce OSM. The possible role of OSM at the start of spermatogenesis was investigated by using a coculture of Sertoli cells and gonocytes isolated from newborn rats. OSM significantly increased the survival of both Sertoli cells and gonocytes in a dose-dependent manner. The proliferative activity of the Sertoli cells was not affected by OSM, whereas that of gonocytes was increased by almost 60% after 6 days of culture. Comparison of the effect of OSM on these cocultures with other members of the interleukin 6 family of cytokines demonstrated that this factor is more potent than leukemia inhibitory factor or ciliary neurotrophic factor. On the basis of these findings, it can be concluded that OSM is present in the rat testis, and it is likely to play an important role at the start of spermatogenesis. PMID: [9149912]
Figures for illustrating the function of this protein/gene
Function
Growth regulator. Inhibits the proliferation of a numberof tumor cell lines. It regulates cytokine production, includingIL-6, G-CSF and GM-CSF from endothelial cells (By similarity).Uses only type II OSM receptor (heterodimers composed of OSMR andIL6ST). Involved in the maturation of fetal hepatocytes, therebypromoting liver development and regeneration.
SIGNAL 1 25 Potential. CHAIN 26 207 Oncostatin-M. /FTId=PRO_0000408765. PROPEP 208 239 By similarity. /FTId=PRO_0000408766. DISULFID 29 140 By similarity. DISULFID 72 179 By similarity. Back to Top